Experiment: R2A_PIPES with Cadmium chloride 128 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt truD and mgtE are separated by 159 nucleotides mgtE and ispF overlap by 4 nucleotides ispF and ispD are separated by 10 nucleotides
OKGIIK_08130: truD - tRNA pseudouridine(13) synthase TruD, at 1,787,515 to 1,788,531
truD
OKGIIK_08135: mgtE - magnesium transporter, at 1,788,691 to 1,790,019
mgtE
OKGIIK_08140: ispF - 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase, at 1,790,016 to 1,790,489
ispF
OKGIIK_08145: ispD - 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase, at 1,790,500 to 1,791,198
ispD
Position (kb)
1788
1789
1790
1791 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1787.938 kb on + strand, within truD at 1787.938 kb on + strand, within truD at 1787.938 kb on + strand, within truD at 1787.938 kb on + strand, within truD at 1787.939 kb on - strand, within truD at 1787.939 kb on - strand, within truD at 1787.939 kb on - strand, within truD at 1787.939 kb on - strand, within truD at 1787.939 kb on - strand, within truD at 1787.939 kb on - strand, within truD at 1788.062 kb on + strand, within truD at 1788.062 kb on + strand, within truD at 1788.062 kb on + strand, within truD at 1788.062 kb on + strand, within truD at 1788.062 kb on + strand, within truD at 1788.062 kb on + strand, within truD at 1788.062 kb on + strand, within truD at 1788.062 kb on + strand, within truD at 1788.062 kb on + strand, within truD at 1788.062 kb on + strand, within truD at 1788.062 kb on + strand, within truD at 1788.063 kb on - strand, within truD at 1788.063 kb on - strand, within truD at 1788.063 kb on - strand, within truD at 1788.063 kb on - strand, within truD at 1788.063 kb on - strand, within truD at 1788.317 kb on + strand, within truD at 1788.317 kb on + strand, within truD at 1788.318 kb on - strand, within truD at 1788.437 kb on + strand at 1788.437 kb on + strand at 1788.438 kb on - strand at 1788.438 kb on - strand at 1788.438 kb on - strand at 1788.516 kb on - strand at 1788.516 kb on - strand at 1788.516 kb on - strand at 1788.625 kb on + strand at 1788.625 kb on + strand at 1788.625 kb on + strand at 1788.625 kb on + strand at 1788.625 kb on + strand at 1788.625 kb on + strand at 1788.659 kb on + strand at 1788.659 kb on + strand at 1788.830 kb on + strand, within mgtE at 1788.830 kb on + strand, within mgtE at 1788.831 kb on - strand, within mgtE at 1788.831 kb on - strand, within mgtE at 1788.831 kb on - strand, within mgtE at 1788.831 kb on - strand, within mgtE at 1788.831 kb on - strand, within mgtE at 1788.831 kb on - strand, within mgtE at 1789.296 kb on - strand, within mgtE at 1789.322 kb on + strand, within mgtE at 1789.322 kb on + strand, within mgtE at 1789.322 kb on + strand, within mgtE at 1789.499 kb on + strand, within mgtE at 1789.500 kb on - strand, within mgtE at 1789.583 kb on + strand, within mgtE at 1789.584 kb on - strand, within mgtE at 1789.584 kb on - strand, within mgtE
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Cadmium chloride 128 uM remove 1,787,938 + truD OKGIIK_08130 0.42 +2.2 1,787,938 + truD OKGIIK_08130 0.42 -0.1 1,787,938 + truD OKGIIK_08130 0.42 +0.9 1,787,938 + truD OKGIIK_08130 0.42 -0.1 1,787,939 - truD OKGIIK_08130 0.42 -1.1 1,787,939 - truD OKGIIK_08130 0.42 -0.8 1,787,939 - truD OKGIIK_08130 0.42 -0.6 1,787,939 - truD OKGIIK_08130 0.42 +0.9 1,787,939 - truD OKGIIK_08130 0.42 +1.0 1,787,939 - truD OKGIIK_08130 0.42 -1.1 1,788,062 + truD OKGIIK_08130 0.54 +0.2 1,788,062 + truD OKGIIK_08130 0.54 -0.9 1,788,062 + truD OKGIIK_08130 0.54 +0.2 1,788,062 + truD OKGIIK_08130 0.54 -1.1 1,788,062 + truD OKGIIK_08130 0.54 +0.6 1,788,062 + truD OKGIIK_08130 0.54 -1.8 1,788,062 + truD OKGIIK_08130 0.54 -1.8 1,788,062 + truD OKGIIK_08130 0.54 -0.3 1,788,062 + truD OKGIIK_08130 0.54 -0.6 1,788,062 + truD OKGIIK_08130 0.54 -0.8 1,788,062 + truD OKGIIK_08130 0.54 -0.4 1,788,063 - truD OKGIIK_08130 0.54 +1.5 1,788,063 - truD OKGIIK_08130 0.54 -1.1 1,788,063 - truD OKGIIK_08130 0.54 -0.3 1,788,063 - truD OKGIIK_08130 0.54 -0.4 1,788,063 - truD OKGIIK_08130 0.54 -0.6 1,788,317 + truD OKGIIK_08130 0.79 -1.6 1,788,317 + truD OKGIIK_08130 0.79 -0.6 1,788,318 - truD OKGIIK_08130 0.79 -0.7 1,788,437 + -0.2 1,788,437 + -2.6 1,788,438 - -0.2 1,788,438 - -0.4 1,788,438 - -0.1 1,788,516 - +0.4 1,788,516 - -0.9 1,788,516 - -0.4 1,788,625 + -0.6 1,788,625 + -0.8 1,788,625 + +2.9 1,788,625 + -1.5 1,788,625 + -1.7 1,788,625 + -1.1 1,788,659 + +2.0 1,788,659 + -1.4 1,788,830 + mgtE OKGIIK_08135 0.10 -0.6 1,788,830 + mgtE OKGIIK_08135 0.10 -0.6 1,788,831 - mgtE OKGIIK_08135 0.11 -1.7 1,788,831 - mgtE OKGIIK_08135 0.11 -0.7 1,788,831 - mgtE OKGIIK_08135 0.11 -0.8 1,788,831 - mgtE OKGIIK_08135 0.11 -0.5 1,788,831 - mgtE OKGIIK_08135 0.11 +2.6 1,788,831 - mgtE OKGIIK_08135 0.11 -0.4 1,789,296 - mgtE OKGIIK_08135 0.46 -2.1 1,789,322 + mgtE OKGIIK_08135 0.47 -0.1 1,789,322 + mgtE OKGIIK_08135 0.47 +0.2 1,789,322 + mgtE OKGIIK_08135 0.47 -1.2 1,789,499 + mgtE OKGIIK_08135 0.61 -0.1 1,789,500 - mgtE OKGIIK_08135 0.61 -0.4 1,789,583 + mgtE OKGIIK_08135 0.67 +0.6 1,789,584 - mgtE OKGIIK_08135 0.67 -0.9 1,789,584 - mgtE OKGIIK_08135 0.67 -0.9
Or see this region's nucleotide sequence