Experiment: R2A_PIPES with Cadmium chloride 128 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt folD and OKGIIK_07105 are separated by 64 nucleotides OKGIIK_07105 and moeB overlap by 4 nucleotides
OKGIIK_07100: folD - bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase FolD, at 1,571,412 to 1,572,281
folD
OKGIIK_07105: OKGIIK_07105 - Ribosomal-protein-serine acetyltransferase, at 1,572,346 to 1,572,915
_07105
OKGIIK_07110: moeB - molybdopterin-synthase adenylyltransferase MoeB, at 1,572,912 to 1,574,081
moeB
Position (kb)
1572
1573 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1571.348 kb on - strand at 1571.348 kb on - strand at 1571.348 kb on - strand at 1571.348 kb on - strand at 1571.348 kb on - strand at 1571.348 kb on - strand at 1571.348 kb on - strand at 1571.348 kb on - strand at 1571.348 kb on - strand at 1571.349 kb on + strand at 1571.349 kb on + strand at 1571.349 kb on + strand at 1571.350 kb on - strand at 1571.350 kb on - strand at 1571.350 kb on - strand at 1571.350 kb on - strand at 1571.350 kb on - strand at 1571.350 kb on - strand at 1571.350 kb on - strand at 1571.350 kb on - strand at 1571.350 kb on - strand at 1572.298 kb on - strand at 1572.502 kb on + strand, within OKGIIK_07105 at 1572.502 kb on + strand, within OKGIIK_07105 at 1572.502 kb on + strand, within OKGIIK_07105 at 1572.502 kb on + strand, within OKGIIK_07105 at 1572.503 kb on - strand, within OKGIIK_07105 at 1572.503 kb on - strand, within OKGIIK_07105 at 1572.886 kb on + strand at 1572.887 kb on - strand at 1572.913 kb on + strand at 1572.913 kb on + strand at 1572.913 kb on + strand at 1572.961 kb on - strand at 1573.273 kb on + strand, within moeB at 1573.273 kb on + strand, within moeB at 1573.273 kb on + strand, within moeB at 1573.273 kb on + strand, within moeB at 1573.273 kb on + strand, within moeB at 1573.273 kb on + strand, within moeB at 1573.273 kb on + strand, within moeB at 1573.274 kb on - strand, within moeB at 1573.274 kb on - strand, within moeB at 1573.274 kb on - strand, within moeB at 1573.274 kb on - strand, within moeB at 1573.274 kb on - strand, within moeB at 1573.274 kb on - strand, within moeB at 1573.274 kb on - strand, within moeB at 1573.274 kb on - strand, within moeB at 1573.318 kb on + strand, within moeB at 1573.319 kb on - strand, within moeB at 1573.580 kb on - strand, within moeB at 1573.580 kb on - strand, within moeB at 1573.580 kb on - strand, within moeB at 1573.580 kb on - strand, within moeB at 1573.580 kb on - strand, within moeB at 1573.580 kb on - strand, within moeB at 1573.580 kb on - strand, within moeB at 1573.580 kb on - strand, within moeB at 1573.690 kb on + strand, within moeB
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Cadmium chloride 128 uM remove 1,571,348 - -0.4 1,571,348 - +1.3 1,571,348 - +1.7 1,571,348 - -1.6 1,571,348 - -0.5 1,571,348 - +0.5 1,571,348 - -0.5 1,571,348 - +0.8 1,571,348 - -0.9 1,571,349 + -1.7 1,571,349 + -1.5 1,571,349 + -1.5 1,571,350 - -1.6 1,571,350 - +0.3 1,571,350 - -0.2 1,571,350 - -1.6 1,571,350 - -1.8 1,571,350 - +0.0 1,571,350 - -0.3 1,571,350 - -1.5 1,571,350 - +0.2 1,572,298 - -0.3 1,572,502 + OKGIIK_07105 0.27 -2.8 1,572,502 + OKGIIK_07105 0.27 -0.1 1,572,502 + OKGIIK_07105 0.27 -2.1 1,572,502 + OKGIIK_07105 0.27 -1.2 1,572,503 - OKGIIK_07105 0.28 +0.3 1,572,503 - OKGIIK_07105 0.28 -0.5 1,572,886 + -0.5 1,572,887 - +0.7 1,572,913 + +0.9 1,572,913 + -0.7 1,572,913 + -0.9 1,572,961 - -0.3 1,573,273 + moeB OKGIIK_07110 0.31 +0.2 1,573,273 + moeB OKGIIK_07110 0.31 +0.3 1,573,273 + moeB OKGIIK_07110 0.31 +0.8 1,573,273 + moeB OKGIIK_07110 0.31 -0.9 1,573,273 + moeB OKGIIK_07110 0.31 +1.7 1,573,273 + moeB OKGIIK_07110 0.31 -0.9 1,573,273 + moeB OKGIIK_07110 0.31 -0.1 1,573,274 - moeB OKGIIK_07110 0.31 -0.7 1,573,274 - moeB OKGIIK_07110 0.31 +1.0 1,573,274 - moeB OKGIIK_07110 0.31 +1.3 1,573,274 - moeB OKGIIK_07110 0.31 +1.9 1,573,274 - moeB OKGIIK_07110 0.31 +0.7 1,573,274 - moeB OKGIIK_07110 0.31 +0.0 1,573,274 - moeB OKGIIK_07110 0.31 -1.3 1,573,274 - moeB OKGIIK_07110 0.31 -0.5 1,573,318 + moeB OKGIIK_07110 0.35 -0.9 1,573,319 - moeB OKGIIK_07110 0.35 -0.3 1,573,580 - moeB OKGIIK_07110 0.57 +0.3 1,573,580 - moeB OKGIIK_07110 0.57 -0.1 1,573,580 - moeB OKGIIK_07110 0.57 -0.5 1,573,580 - moeB OKGIIK_07110 0.57 -1.6 1,573,580 - moeB OKGIIK_07110 0.57 +0.3 1,573,580 - moeB OKGIIK_07110 0.57 -0.1 1,573,580 - moeB OKGIIK_07110 0.57 -0.5 1,573,580 - moeB OKGIIK_07110 0.57 -0.7 1,573,690 + moeB OKGIIK_07110 0.66 +1.3
Or see this region's nucleotide sequence