Experiment: R2A_PIPES with Cadmium chloride 128 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt recJ and OKGIIK_07000 are separated by 75 nucleotides OKGIIK_07000 and asnB are separated by 327 nucleotides asnB and OKGIIK_07010 are separated by 42 nucleotides OKGIIK_07010 and prfB are separated by 58 nucleotides
OKGIIK_06995: recJ - single-stranded-DNA-specific exonuclease RecJ, at 1,550,465 to 1,552,336
recJ
OKGIIK_07000: OKGIIK_07000 - SpoIIAA-like, at 1,552,412 to 1,552,789
_07000
OKGIIK_07005: asnB - asparagine synthase B, at 1,553,117 to 1,554,784
asnB
OKGIIK_07010: OKGIIK_07010 - hypothetical protein, at 1,554,827 to 1,554,958
_07010
OKGIIK_07015: prfB - peptide chain release factor 2, at 1,555,017 to 1,556,006
prfB
Position (kb)
1553
1554
1555 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1552.223 kb on + strand at 1552.223 kb on + strand at 1552.223 kb on + strand at 1552.223 kb on + strand at 1552.223 kb on + strand at 1552.223 kb on + strand at 1552.224 kb on - strand at 1552.224 kb on - strand at 1552.224 kb on - strand at 1552.224 kb on - strand at 1552.224 kb on - strand at 1552.224 kb on - strand at 1552.224 kb on - strand at 1552.262 kb on + strand at 1552.263 kb on - strand at 1552.263 kb on - strand at 1552.319 kb on + strand at 1552.319 kb on + strand at 1552.319 kb on + strand at 1552.319 kb on + strand at 1552.319 kb on + strand at 1552.319 kb on + strand at 1552.319 kb on + strand at 1552.319 kb on + strand at 1552.319 kb on + strand at 1552.320 kb on - strand at 1552.320 kb on - strand at 1552.320 kb on - strand at 1552.320 kb on - strand at 1552.320 kb on - strand at 1552.320 kb on - strand at 1552.320 kb on - strand at 1552.320 kb on - strand at 1552.320 kb on - strand at 1552.320 kb on - strand at 1552.342 kb on + strand at 1552.354 kb on + strand at 1552.354 kb on + strand at 1552.354 kb on + strand at 1552.488 kb on - strand, within OKGIIK_07000 at 1552.488 kb on - strand, within OKGIIK_07000 at 1552.488 kb on - strand, within OKGIIK_07000 at 1552.553 kb on + strand, within OKGIIK_07000 at 1552.553 kb on + strand, within OKGIIK_07000 at 1552.553 kb on + strand, within OKGIIK_07000 at 1552.553 kb on + strand, within OKGIIK_07000 at 1552.553 kb on + strand, within OKGIIK_07000 at 1552.553 kb on + strand, within OKGIIK_07000 at 1552.553 kb on + strand, within OKGIIK_07000 at 1552.553 kb on + strand, within OKGIIK_07000 at 1552.554 kb on - strand, within OKGIIK_07000 at 1552.554 kb on - strand, within OKGIIK_07000 at 1552.554 kb on - strand, within OKGIIK_07000 at 1552.554 kb on - strand, within OKGIIK_07000 at 1552.554 kb on - strand, within OKGIIK_07000 at 1552.554 kb on - strand, within OKGIIK_07000 at 1552.554 kb on - strand, within OKGIIK_07000 at 1552.554 kb on - strand, within OKGIIK_07000 at 1552.554 kb on - strand, within OKGIIK_07000 at 1552.554 kb on - strand, within OKGIIK_07000 at 1552.554 kb on - strand, within OKGIIK_07000 at 1552.913 kb on - strand at 1552.913 kb on - strand at 1552.913 kb on - strand at 1552.913 kb on - strand at 1552.913 kb on - strand at 1552.928 kb on - strand at 1552.956 kb on - strand at 1553.096 kb on + strand at 1553.357 kb on + strand, within asnB at 1553.357 kb on + strand, within asnB at 1553.390 kb on + strand, within asnB at 1553.390 kb on + strand, within asnB at 1553.435 kb on + strand, within asnB at 1553.436 kb on - strand, within asnB at 1553.454 kb on - strand, within asnB at 1553.454 kb on - strand, within asnB at 1553.454 kb on - strand, within asnB at 1553.454 kb on - strand, within asnB at 1553.454 kb on - strand, within asnB at 1553.454 kb on - strand, within asnB at 1553.454 kb on - strand, within asnB at 1553.516 kb on + strand, within asnB at 1553.555 kb on + strand, within asnB at 1553.555 kb on + strand, within asnB at 1553.556 kb on - strand, within asnB at 1553.556 kb on - strand, within asnB at 1553.657 kb on + strand, within asnB at 1553.658 kb on - strand, within asnB at 1553.687 kb on + strand, within asnB at 1553.688 kb on - strand, within asnB at 1553.688 kb on - strand, within asnB at 1553.688 kb on - strand, within asnB at 1553.688 kb on - strand, within asnB at 1553.690 kb on + strand, within asnB at 1553.691 kb on - strand, within asnB at 1553.691 kb on - strand, within asnB at 1554.014 kb on + strand, within asnB at 1554.071 kb on + strand, within asnB at 1554.072 kb on - strand, within asnB at 1554.072 kb on - strand, within asnB at 1554.107 kb on + strand, within asnB at 1554.107 kb on + strand, within asnB at 1554.107 kb on + strand, within asnB at 1554.108 kb on - strand, within asnB at 1554.108 kb on - strand, within asnB at 1554.108 kb on - strand, within asnB at 1554.194 kb on + strand, within asnB at 1554.194 kb on + strand, within asnB at 1554.195 kb on - strand, within asnB at 1554.195 kb on - strand, within asnB at 1554.195 kb on - strand, within asnB at 1554.195 kb on - strand, within asnB at 1554.266 kb on + strand, within asnB at 1554.266 kb on + strand, within asnB at 1554.266 kb on + strand, within asnB at 1554.267 kb on - strand, within asnB at 1554.267 kb on - strand, within asnB at 1554.267 kb on - strand, within asnB at 1554.267 kb on - strand, within asnB at 1554.267 kb on - strand, within asnB at 1554.602 kb on + strand, within asnB at 1554.602 kb on + strand, within asnB at 1554.602 kb on + strand, within asnB at 1554.602 kb on + strand, within asnB at 1554.608 kb on + strand, within asnB at 1554.608 kb on + strand, within asnB at 1554.609 kb on - strand, within asnB at 1554.609 kb on - strand, within asnB at 1554.806 kb on + strand at 1554.806 kb on + strand at 1554.806 kb on + strand at 1554.806 kb on + strand at 1554.806 kb on + strand at 1554.806 kb on + strand at 1554.806 kb on + strand at 1554.806 kb on + strand at 1554.807 kb on - strand at 1554.807 kb on - strand at 1554.807 kb on - strand at 1554.807 kb on - strand at 1554.807 kb on - strand at 1554.807 kb on - strand at 1554.807 kb on - strand at 1554.807 kb on - strand at 1554.807 kb on - strand at 1554.807 kb on - strand at 1554.807 kb on - strand at 1554.826 kb on + strand at 1554.827 kb on - strand at 1554.827 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Cadmium chloride 128 uM remove 1,552,223 + +0.3 1,552,223 + -1.7 1,552,223 + -1.6 1,552,223 + -0.7 1,552,223 + -1.5 1,552,223 + +1.1 1,552,224 - -0.0 1,552,224 - -0.7 1,552,224 - -0.8 1,552,224 - -0.3 1,552,224 - +1.3 1,552,224 - -0.9 1,552,224 - -1.9 1,552,262 + +1.3 1,552,263 - -1.5 1,552,263 - -0.3 1,552,319 + +1.3 1,552,319 + -0.0 1,552,319 + -0.0 1,552,319 + -0.3 1,552,319 + -0.0 1,552,319 + -1.7 1,552,319 + -0.5 1,552,319 + +1.7 1,552,319 + +1.9 1,552,320 - +0.5 1,552,320 - +2.3 1,552,320 - +1.0 1,552,320 - -0.3 1,552,320 - +0.5 1,552,320 - -1.4 1,552,320 - -1.0 1,552,320 - -1.3 1,552,320 - -0.5 1,552,320 - +0.3 1,552,342 + +0.7 1,552,354 + -0.7 1,552,354 + -1.1 1,552,354 + -0.1 1,552,488 - OKGIIK_07000 0.20 -0.9 1,552,488 - OKGIIK_07000 0.20 -0.9 1,552,488 - OKGIIK_07000 0.20 +1.0 1,552,553 + OKGIIK_07000 0.37 +0.3 1,552,553 + OKGIIK_07000 0.37 -1.0 1,552,553 + OKGIIK_07000 0.37 +1.7 1,552,553 + OKGIIK_07000 0.37 -0.6 1,552,553 + OKGIIK_07000 0.37 -0.0 1,552,553 + OKGIIK_07000 0.37 -3.0 1,552,553 + OKGIIK_07000 0.37 +1.3 1,552,553 + OKGIIK_07000 0.37 +0.3 1,552,554 - OKGIIK_07000 0.38 -2.5 1,552,554 - OKGIIK_07000 0.38 +0.2 1,552,554 - OKGIIK_07000 0.38 -0.9 1,552,554 - OKGIIK_07000 0.38 +1.3 1,552,554 - OKGIIK_07000 0.38 -0.9 1,552,554 - OKGIIK_07000 0.38 -0.3 1,552,554 - OKGIIK_07000 0.38 +0.5 1,552,554 - OKGIIK_07000 0.38 -0.3 1,552,554 - OKGIIK_07000 0.38 +0.5 1,552,554 - OKGIIK_07000 0.38 +0.4 1,552,554 - OKGIIK_07000 0.38 -0.3 1,552,913 - -0.3 1,552,913 - -0.0 1,552,913 - +0.3 1,552,913 - +0.7 1,552,913 - +0.7 1,552,928 - -0.9 1,552,956 - -0.6 1,553,096 + +0.3 1,553,357 + asnB OKGIIK_07005 0.14 -1.5 1,553,357 + asnB OKGIIK_07005 0.14 +1.5 1,553,390 + asnB OKGIIK_07005 0.16 -0.5 1,553,390 + asnB OKGIIK_07005 0.16 -0.0 1,553,435 + asnB OKGIIK_07005 0.19 -0.0 1,553,436 - asnB OKGIIK_07005 0.19 -0.0 1,553,454 - asnB OKGIIK_07005 0.20 +0.3 1,553,454 - asnB OKGIIK_07005 0.20 +0.7 1,553,454 - asnB OKGIIK_07005 0.20 -0.0 1,553,454 - asnB OKGIIK_07005 0.20 +1.5 1,553,454 - asnB OKGIIK_07005 0.20 -0.7 1,553,454 - asnB OKGIIK_07005 0.20 -0.5 1,553,454 - asnB OKGIIK_07005 0.20 -0.3 1,553,516 + asnB OKGIIK_07005 0.24 -0.0 1,553,555 + asnB OKGIIK_07005 0.26 -0.0 1,553,555 + asnB OKGIIK_07005 0.26 +0.3 1,553,556 - asnB OKGIIK_07005 0.26 -0.0 1,553,556 - asnB OKGIIK_07005 0.26 +1.3 1,553,657 + asnB OKGIIK_07005 0.32 -0.3 1,553,658 - asnB OKGIIK_07005 0.32 +0.3 1,553,687 + asnB OKGIIK_07005 0.34 +0.3 1,553,688 - asnB OKGIIK_07005 0.34 -0.0 1,553,688 - asnB OKGIIK_07005 0.34 -0.3 1,553,688 - asnB OKGIIK_07005 0.34 +0.2 1,553,688 - asnB OKGIIK_07005 0.34 -0.7 1,553,690 + asnB OKGIIK_07005 0.34 -1.4 1,553,691 - asnB OKGIIK_07005 0.34 +1.5 1,553,691 - asnB OKGIIK_07005 0.34 -0.9 1,554,014 + asnB OKGIIK_07005 0.54 -0.7 1,554,071 + asnB OKGIIK_07005 0.57 +1.5 1,554,072 - asnB OKGIIK_07005 0.57 -0.3 1,554,072 - asnB OKGIIK_07005 0.57 -0.3 1,554,107 + asnB OKGIIK_07005 0.59 -0.5 1,554,107 + asnB OKGIIK_07005 0.59 -0.7 1,554,107 + asnB OKGIIK_07005 0.59 +0.1 1,554,108 - asnB OKGIIK_07005 0.59 -0.5 1,554,108 - asnB OKGIIK_07005 0.59 +1.3 1,554,108 - asnB OKGIIK_07005 0.59 +0.7 1,554,194 + asnB OKGIIK_07005 0.65 +1.8 1,554,194 + asnB OKGIIK_07005 0.65 -0.9 1,554,195 - asnB OKGIIK_07005 0.65 +1.9 1,554,195 - asnB OKGIIK_07005 0.65 -0.0 1,554,195 - asnB OKGIIK_07005 0.65 +1.3 1,554,195 - asnB OKGIIK_07005 0.65 -1.0 1,554,266 + asnB OKGIIK_07005 0.69 -1.7 1,554,266 + asnB OKGIIK_07005 0.69 +1.0 1,554,266 + asnB OKGIIK_07005 0.69 -1.3 1,554,267 - asnB OKGIIK_07005 0.69 -0.0 1,554,267 - asnB OKGIIK_07005 0.69 +0.3 1,554,267 - asnB OKGIIK_07005 0.69 +0.3 1,554,267 - asnB OKGIIK_07005 0.69 -0.3 1,554,267 - asnB OKGIIK_07005 0.69 -0.0 1,554,602 + asnB OKGIIK_07005 0.89 -0.7 1,554,602 + asnB OKGIIK_07005 0.89 -0.7 1,554,602 + asnB OKGIIK_07005 0.89 +0.3 1,554,602 + asnB OKGIIK_07005 0.89 -0.1 1,554,608 + asnB OKGIIK_07005 0.89 -0.5 1,554,608 + asnB OKGIIK_07005 0.89 -2.0 1,554,609 - asnB OKGIIK_07005 0.89 -0.8 1,554,609 - asnB OKGIIK_07005 0.89 -0.5 1,554,806 + -1.6 1,554,806 + -1.5 1,554,806 + +0.7 1,554,806 + +0.8 1,554,806 + -1.2 1,554,806 + +0.3 1,554,806 + -1.0 1,554,806 + -0.5 1,554,807 - -1.0 1,554,807 - -0.3 1,554,807 - -0.3 1,554,807 - -0.1 1,554,807 - -0.5 1,554,807 - +1.2 1,554,807 - -1.0 1,554,807 - -0.7 1,554,807 - -0.7 1,554,807 - -0.4 1,554,807 - -0.4 1,554,826 + -2.6 1,554,827 - +0.8 1,554,827 - -0.7
Or see this region's nucleotide sequence