Experiment: R2A_PIPES with Cadmium chloride 128 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt OKGIIK_04220 and bcp are separated by 82 nucleotides bcp and OKGIIK_04230 are separated by 227 nucleotides OKGIIK_04230 and OKGIIK_04235 are separated by 158 nucleotides
OKGIIK_04220: OKGIIK_04220 - Peptidase, at 922,391 to 923,968
_04220
OKGIIK_04225: bcp - Peroxiredoxin, at 924,051 to 924,602
bcp
OKGIIK_04230: OKGIIK_04230 - hypothetical protein, at 924,830 to 925,216
_04230
OKGIIK_04235: OKGIIK_04235 - Diguanylate cyclase, at 925,375 to 927,156
_04235
Position (kb) 924
925
926 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 923.913 kb on - strand at 924.022 kb on + strand at 924.022 kb on + strand at 924.022 kb on + strand at 924.022 kb on + strand at 924.022 kb on + strand at 924.022 kb on + strand at 924.022 kb on + strand at 924.022 kb on + strand at 924.022 kb on + strand at 924.022 kb on + strand at 924.052 kb on + strand at 924.124 kb on + strand, within bcp at 924.124 kb on + strand, within bcp at 924.124 kb on + strand, within bcp at 924.124 kb on + strand, within bcp at 924.125 kb on - strand, within bcp at 924.125 kb on - strand, within bcp at 924.160 kb on + strand, within bcp at 924.160 kb on + strand, within bcp at 924.160 kb on + strand, within bcp at 924.160 kb on + strand, within bcp at 924.160 kb on + strand, within bcp at 924.160 kb on + strand, within bcp at 924.160 kb on + strand, within bcp at 924.160 kb on + strand, within bcp at 924.160 kb on + strand, within bcp at 924.160 kb on + strand, within bcp at 924.160 kb on + strand, within bcp at 924.160 kb on + strand, within bcp at 924.160 kb on + strand, within bcp at 924.160 kb on + strand, within bcp at 924.160 kb on + strand, within bcp at 924.161 kb on - strand, within bcp at 924.161 kb on - strand, within bcp at 924.161 kb on - strand, within bcp at 924.161 kb on - strand, within bcp at 924.161 kb on - strand, within bcp at 924.161 kb on - strand, within bcp at 924.161 kb on - strand, within bcp at 924.208 kb on + strand, within bcp at 924.208 kb on + strand, within bcp at 924.208 kb on + strand, within bcp at 924.208 kb on + strand, within bcp at 924.209 kb on - strand, within bcp at 924.302 kb on + strand, within bcp at 924.303 kb on - strand, within bcp at 924.418 kb on + strand, within bcp at 924.418 kb on + strand, within bcp at 924.418 kb on + strand, within bcp at 924.418 kb on + strand, within bcp at 924.418 kb on + strand, within bcp at 924.418 kb on + strand, within bcp at 924.418 kb on + strand, within bcp at 924.418 kb on + strand, within bcp at 924.418 kb on + strand, within bcp at 924.418 kb on + strand, within bcp at 924.418 kb on + strand, within bcp at 924.418 kb on + strand, within bcp at 924.418 kb on + strand, within bcp at 924.418 kb on + strand, within bcp at 924.419 kb on - strand, within bcp at 924.419 kb on - strand, within bcp at 924.419 kb on - strand, within bcp at 924.419 kb on - strand, within bcp at 924.419 kb on - strand, within bcp at 924.419 kb on - strand, within bcp at 924.419 kb on - strand, within bcp at 924.419 kb on - strand, within bcp at 924.419 kb on - strand, within bcp at 924.419 kb on - strand, within bcp at 924.419 kb on - strand, within bcp at 924.419 kb on - strand, within bcp at 924.419 kb on - strand, within bcp at 924.419 kb on - strand, within bcp at 924.419 kb on - strand, within bcp at 924.419 kb on - strand, within bcp at 924.419 kb on - strand, within bcp at 924.419 kb on - strand, within bcp at 924.485 kb on - strand, within bcp at 924.485 kb on - strand, within bcp at 924.485 kb on - strand, within bcp at 924.485 kb on - strand, within bcp at 924.636 kb on + strand at 924.637 kb on - strand at 924.637 kb on - strand at 924.637 kb on - strand at 924.638 kb on + strand at 924.639 kb on - strand at 924.639 kb on - strand at 924.766 kb on + strand at 924.766 kb on + strand at 924.766 kb on + strand at 924.766 kb on + strand at 924.766 kb on + strand at 924.766 kb on + strand at 924.766 kb on + strand at 924.766 kb on + strand at 924.766 kb on + strand at 924.766 kb on + strand at 924.766 kb on + strand at 924.766 kb on + strand at 924.767 kb on - strand at 924.808 kb on + strand at 924.808 kb on + strand at 925.123 kb on + strand, within OKGIIK_04230 at 925.123 kb on + strand, within OKGIIK_04230 at 925.123 kb on + strand, within OKGIIK_04230 at 925.123 kb on + strand, within OKGIIK_04230 at 925.123 kb on + strand, within OKGIIK_04230 at 925.124 kb on - strand, within OKGIIK_04230 at 925.124 kb on - strand, within OKGIIK_04230 at 925.124 kb on - strand, within OKGIIK_04230 at 925.124 kb on - strand, within OKGIIK_04230 at 925.124 kb on - strand, within OKGIIK_04230 at 925.124 kb on - strand, within OKGIIK_04230 at 925.124 kb on - strand, within OKGIIK_04230 at 925.124 kb on - strand, within OKGIIK_04230 at 925.124 kb on - strand, within OKGIIK_04230 at 925.311 kb on - strand at 925.445 kb on + strand at 925.534 kb on + strand at 925.534 kb on + strand at 925.534 kb on + strand at 925.534 kb on + strand at 925.534 kb on + strand at 925.534 kb on + strand at 925.534 kb on + strand at 925.534 kb on + strand at 925.534 kb on + strand at 925.534 kb on + strand at 925.534 kb on + strand at 925.535 kb on - strand at 925.535 kb on - strand at 925.535 kb on - strand at 925.535 kb on - strand at 925.681 kb on + strand, within OKGIIK_04235 at 925.681 kb on + strand, within OKGIIK_04235 at 925.681 kb on + strand, within OKGIIK_04235 at 925.681 kb on + strand, within OKGIIK_04235 at 925.682 kb on - strand, within OKGIIK_04235 at 925.682 kb on - strand, within OKGIIK_04235 at 925.682 kb on - strand, within OKGIIK_04235 at 925.783 kb on + strand, within OKGIIK_04235 at 925.783 kb on + strand, within OKGIIK_04235 at 925.783 kb on + strand, within OKGIIK_04235 at 925.783 kb on + strand, within OKGIIK_04235 at 925.783 kb on + strand, within OKGIIK_04235 at 925.976 kb on + strand, within OKGIIK_04235 at 925.976 kb on + strand, within OKGIIK_04235 at 925.976 kb on + strand, within OKGIIK_04235 at 925.976 kb on + strand, within OKGIIK_04235 at 925.976 kb on + strand, within OKGIIK_04235 at 925.977 kb on - strand, within OKGIIK_04235 at 925.977 kb on - strand, within OKGIIK_04235 at 925.977 kb on - strand, within OKGIIK_04235 at 925.977 kb on - strand, within OKGIIK_04235 at 925.977 kb on - strand, within OKGIIK_04235 at 925.977 kb on - strand, within OKGIIK_04235 at 926.099 kb on + strand, within OKGIIK_04235 at 926.100 kb on - strand, within OKGIIK_04235 at 926.153 kb on + strand, within OKGIIK_04235 at 926.153 kb on + strand, within OKGIIK_04235 at 926.153 kb on + strand, within OKGIIK_04235 at 926.153 kb on + strand, within OKGIIK_04235 at 926.153 kb on + strand, within OKGIIK_04235 at 926.153 kb on + strand, within OKGIIK_04235 at 926.153 kb on + strand, within OKGIIK_04235 at 926.153 kb on + strand, within OKGIIK_04235 at 926.153 kb on + strand, within OKGIIK_04235 at 926.153 kb on + strand, within OKGIIK_04235 at 926.153 kb on + strand, within OKGIIK_04235 at 926.153 kb on + strand, within OKGIIK_04235 at 926.153 kb on + strand, within OKGIIK_04235 at 926.153 kb on + strand, within OKGIIK_04235 at 926.153 kb on + strand, within OKGIIK_04235 at 926.153 kb on + strand, within OKGIIK_04235 at 926.153 kb on + strand, within OKGIIK_04235 at 926.153 kb on + strand, within OKGIIK_04235 at 926.153 kb on + strand, within OKGIIK_04235 at 926.153 kb on + strand, within OKGIIK_04235 at 926.153 kb on + strand, within OKGIIK_04235 at 926.153 kb on + strand, within OKGIIK_04235 at 926.154 kb on - strand, within OKGIIK_04235 at 926.154 kb on - strand, within OKGIIK_04235 at 926.154 kb on - strand, within OKGIIK_04235 at 926.154 kb on - strand, within OKGIIK_04235 at 926.154 kb on - strand, within OKGIIK_04235 at 926.154 kb on - strand, within OKGIIK_04235 at 926.154 kb on - strand, within OKGIIK_04235 at 926.154 kb on - strand, within OKGIIK_04235 at 926.154 kb on - strand, within OKGIIK_04235 at 926.154 kb on - strand, within OKGIIK_04235 at 926.154 kb on - strand, within OKGIIK_04235 at 926.154 kb on - strand, within OKGIIK_04235 at 926.154 kb on - strand, within OKGIIK_04235 at 926.154 kb on - strand, within OKGIIK_04235 at 926.154 kb on - strand, within OKGIIK_04235 at 926.154 kb on - strand, within OKGIIK_04235 at 926.154 kb on - strand, within OKGIIK_04235 at 926.154 kb on - strand, within OKGIIK_04235 at 926.154 kb on - strand, within OKGIIK_04235 at 926.154 kb on - strand, within OKGIIK_04235 at 926.162 kb on + strand, within OKGIIK_04235 at 926.162 kb on + strand, within OKGIIK_04235 at 926.162 kb on + strand, within OKGIIK_04235 at 926.163 kb on - strand, within OKGIIK_04235 at 926.163 kb on - strand, within OKGIIK_04235 at 926.163 kb on - strand, within OKGIIK_04235 at 926.165 kb on + strand, within OKGIIK_04235 at 926.166 kb on - strand, within OKGIIK_04235 at 926.166 kb on - strand, within OKGIIK_04235 at 926.166 kb on - strand, within OKGIIK_04235 at 926.211 kb on - strand, within OKGIIK_04235
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Cadmium chloride 128 uM remove 923,913 - -1.2 924,022 + -1.1 924,022 + -0.3 924,022 + -0.6 924,022 + -1.1 924,022 + -0.7 924,022 + +0.1 924,022 + +0.4 924,022 + +1.1 924,022 + +0.4 924,022 + -0.8 924,052 + -0.1 924,124 + bcp OKGIIK_04225 0.13 +0.7 924,124 + bcp OKGIIK_04225 0.13 +0.7 924,124 + bcp OKGIIK_04225 0.13 -0.1 924,124 + bcp OKGIIK_04225 0.13 -0.9 924,125 - bcp OKGIIK_04225 0.13 +0.8 924,125 - bcp OKGIIK_04225 0.13 -0.3 924,160 + bcp OKGIIK_04225 0.20 -0.6 924,160 + bcp OKGIIK_04225 0.20 -2.7 924,160 + bcp OKGIIK_04225 0.20 +1.2 924,160 + bcp OKGIIK_04225 0.20 -1.2 924,160 + bcp OKGIIK_04225 0.20 -2.3 924,160 + bcp OKGIIK_04225 0.20 -0.9 924,160 + bcp OKGIIK_04225 0.20 +1.1 924,160 + bcp OKGIIK_04225 0.20 -0.3 924,160 + bcp OKGIIK_04225 0.20 +0.2 924,160 + bcp OKGIIK_04225 0.20 +2.1 924,160 + bcp OKGIIK_04225 0.20 -1.5 924,160 + bcp OKGIIK_04225 0.20 -1.7 924,160 + bcp OKGIIK_04225 0.20 +0.8 924,160 + bcp OKGIIK_04225 0.20 +3.2 924,160 + bcp OKGIIK_04225 0.20 -0.8 924,161 - bcp OKGIIK_04225 0.20 -1.3 924,161 - bcp OKGIIK_04225 0.20 +0.2 924,161 - bcp OKGIIK_04225 0.20 -0.5 924,161 - bcp OKGIIK_04225 0.20 -1.7 924,161 - bcp OKGIIK_04225 0.20 -0.1 924,161 - bcp OKGIIK_04225 0.20 +2.4 924,161 - bcp OKGIIK_04225 0.20 -0.0 924,208 + bcp OKGIIK_04225 0.28 -1.6 924,208 + bcp OKGIIK_04225 0.28 +0.3 924,208 + bcp OKGIIK_04225 0.28 -1.6 924,208 + bcp OKGIIK_04225 0.28 -0.3 924,209 - bcp OKGIIK_04225 0.29 -0.3 924,302 + bcp OKGIIK_04225 0.45 +0.4 924,303 - bcp OKGIIK_04225 0.46 +1.0 924,418 + bcp OKGIIK_04225 0.66 -2.1 924,418 + bcp OKGIIK_04225 0.66 +1.4 924,418 + bcp OKGIIK_04225 0.66 -0.2 924,418 + bcp OKGIIK_04225 0.66 +0.5 924,418 + bcp OKGIIK_04225 0.66 +0.1 924,418 + bcp OKGIIK_04225 0.66 -0.0 924,418 + bcp OKGIIK_04225 0.66 +0.7 924,418 + bcp OKGIIK_04225 0.66 +0.4 924,418 + bcp OKGIIK_04225 0.66 +0.1 924,418 + bcp OKGIIK_04225 0.66 -0.9 924,418 + bcp OKGIIK_04225 0.66 +0.8 924,418 + bcp OKGIIK_04225 0.66 -1.8 924,418 + bcp OKGIIK_04225 0.66 -0.3 924,418 + bcp OKGIIK_04225 0.66 +0.2 924,419 - bcp OKGIIK_04225 0.67 -0.6 924,419 - bcp OKGIIK_04225 0.67 +1.0 924,419 - bcp OKGIIK_04225 0.67 -1.4 924,419 - bcp OKGIIK_04225 0.67 +1.2 924,419 - bcp OKGIIK_04225 0.67 -0.4 924,419 - bcp OKGIIK_04225 0.67 -2.3 924,419 - bcp OKGIIK_04225 0.67 +0.7 924,419 - bcp OKGIIK_04225 0.67 -1.3 924,419 - bcp OKGIIK_04225 0.67 +0.5 924,419 - bcp OKGIIK_04225 0.67 +0.7 924,419 - bcp OKGIIK_04225 0.67 +1.7 924,419 - bcp OKGIIK_04225 0.67 -2.0 924,419 - bcp OKGIIK_04225 0.67 -0.6 924,419 - bcp OKGIIK_04225 0.67 -0.6 924,419 - bcp OKGIIK_04225 0.67 -0.5 924,419 - bcp OKGIIK_04225 0.67 +1.1 924,419 - bcp OKGIIK_04225 0.67 -2.3 924,419 - bcp OKGIIK_04225 0.67 -0.8 924,485 - bcp OKGIIK_04225 0.79 -0.6 924,485 - bcp OKGIIK_04225 0.79 -1.2 924,485 - bcp OKGIIK_04225 0.79 -0.1 924,485 - bcp OKGIIK_04225 0.79 -1.2 924,636 + -1.0 924,637 - -0.8 924,637 - +1.7 924,637 - +0.7 924,638 + -0.2 924,639 - -0.1 924,639 - -1.1 924,766 + +0.4 924,766 + +1.0 924,766 + -0.9 924,766 + -0.8 924,766 + -0.6 924,766 + +0.8 924,766 + +0.1 924,766 + -0.9 924,766 + -1.6 924,766 + -1.1 924,766 + -0.3 924,766 + +1.2 924,767 - -0.6 924,808 + -1.0 924,808 + -1.2 925,123 + OKGIIK_04230 0.76 -0.6 925,123 + OKGIIK_04230 0.76 -2.3 925,123 + OKGIIK_04230 0.76 -1.5 925,123 + OKGIIK_04230 0.76 -0.3 925,123 + OKGIIK_04230 0.76 -0.3 925,124 - OKGIIK_04230 0.76 -1.1 925,124 - OKGIIK_04230 0.76 -1.1 925,124 - OKGIIK_04230 0.76 -1.1 925,124 - OKGIIK_04230 0.76 -0.1 925,124 - OKGIIK_04230 0.76 -0.9 925,124 - OKGIIK_04230 0.76 -1.5 925,124 - OKGIIK_04230 0.76 +1.8 925,124 - OKGIIK_04230 0.76 -0.8 925,124 - OKGIIK_04230 0.76 -0.6 925,311 - -1.3 925,445 + -0.6 925,534 + -0.9 925,534 + -1.1 925,534 + +0.4 925,534 + -0.3 925,534 + -0.3 925,534 + -0.8 925,534 + +0.4 925,534 + -0.9 925,534 + -1.5 925,534 + -1.5 925,534 + +0.3 925,535 - +0.2 925,535 - -0.8 925,535 - +1.1 925,535 - -0.6 925,681 + OKGIIK_04235 0.17 -0.3 925,681 + OKGIIK_04235 0.17 +1.2 925,681 + OKGIIK_04235 0.17 -0.0 925,681 + OKGIIK_04235 0.17 -0.9 925,682 - OKGIIK_04235 0.17 +0.0 925,682 - OKGIIK_04235 0.17 -1.3 925,682 - OKGIIK_04235 0.17 +0.9 925,783 + OKGIIK_04235 0.23 -1.2 925,783 + OKGIIK_04235 0.23 -0.2 925,783 + OKGIIK_04235 0.23 +0.1 925,783 + OKGIIK_04235 0.23 +0.2 925,783 + OKGIIK_04235 0.23 +0.7 925,976 + OKGIIK_04235 0.34 +0.2 925,976 + OKGIIK_04235 0.34 -0.1 925,976 + OKGIIK_04235 0.34 +1.2 925,976 + OKGIIK_04235 0.34 +0.2 925,976 + OKGIIK_04235 0.34 -1.1 925,977 - OKGIIK_04235 0.34 -0.9 925,977 - OKGIIK_04235 0.34 -1.5 925,977 - OKGIIK_04235 0.34 +1.1 925,977 - OKGIIK_04235 0.34 -0.3 925,977 - OKGIIK_04235 0.34 -0.3 925,977 - OKGIIK_04235 0.34 +0.4 926,099 + OKGIIK_04235 0.41 -1.4 926,100 - OKGIIK_04235 0.41 -1.1 926,153 + OKGIIK_04235 0.44 -0.1 926,153 + OKGIIK_04235 0.44 -0.8 926,153 + OKGIIK_04235 0.44 -0.6 926,153 + OKGIIK_04235 0.44 -0.6 926,153 + OKGIIK_04235 0.44 +1.9 926,153 + OKGIIK_04235 0.44 -0.0 926,153 + OKGIIK_04235 0.44 -1.9 926,153 + OKGIIK_04235 0.44 +0.8 926,153 + OKGIIK_04235 0.44 -0.9 926,153 + OKGIIK_04235 0.44 -0.3 926,153 + OKGIIK_04235 0.44 -0.3 926,153 + OKGIIK_04235 0.44 -0.9 926,153 + OKGIIK_04235 0.44 +0.5 926,153 + OKGIIK_04235 0.44 -0.4 926,153 + OKGIIK_04235 0.44 -1.6 926,153 + OKGIIK_04235 0.44 -1.3 926,153 + OKGIIK_04235 0.44 -1.1 926,153 + OKGIIK_04235 0.44 +0.7 926,153 + OKGIIK_04235 0.44 -1.3 926,153 + OKGIIK_04235 0.44 -0.0 926,153 + OKGIIK_04235 0.44 -0.8 926,153 + OKGIIK_04235 0.44 +1.2 926,154 - OKGIIK_04235 0.44 +0.8 926,154 - OKGIIK_04235 0.44 -1.3 926,154 - OKGIIK_04235 0.44 +0.5 926,154 - OKGIIK_04235 0.44 +0.7 926,154 - OKGIIK_04235 0.44 -1.3 926,154 - OKGIIK_04235 0.44 +0.6 926,154 - OKGIIK_04235 0.44 +0.5 926,154 - OKGIIK_04235 0.44 +0.1 926,154 - OKGIIK_04235 0.44 +0.2 926,154 - OKGIIK_04235 0.44 -0.3 926,154 - OKGIIK_04235 0.44 -0.2 926,154 - OKGIIK_04235 0.44 -0.0 926,154 - OKGIIK_04235 0.44 +0.1 926,154 - OKGIIK_04235 0.44 -0.1 926,154 - OKGIIK_04235 0.44 +2.2 926,154 - OKGIIK_04235 0.44 +0.2 926,154 - OKGIIK_04235 0.44 +0.1 926,154 - OKGIIK_04235 0.44 +0.6 926,154 - OKGIIK_04235 0.44 +1.2 926,154 - OKGIIK_04235 0.44 -0.1 926,162 + OKGIIK_04235 0.44 +0.2 926,162 + OKGIIK_04235 0.44 +0.7 926,162 + OKGIIK_04235 0.44 -1.1 926,163 - OKGIIK_04235 0.44 -0.7 926,163 - OKGIIK_04235 0.44 +0.4 926,163 - OKGIIK_04235 0.44 +0.0 926,165 + OKGIIK_04235 0.44 -0.1 926,166 - OKGIIK_04235 0.44 -1.0 926,166 - OKGIIK_04235 0.44 -1.8 926,166 - OKGIIK_04235 0.44 +0.2 926,211 - OKGIIK_04235 0.47 -0.0
Or see this region's nucleotide sequence