Experiment: R2A_PIPES with Cadmium chloride 128 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt OKGIIK_04110 and OKGIIK_04115 are separated by 47 nucleotides OKGIIK_04115 and OKGIIK_04120 are separated by 330 nucleotides OKGIIK_04120 and OKGIIK_04130 are separated by 519 nucleotides
OKGIIK_04110: OKGIIK_04110 - IS3 family transposase, at 901,497 to 902,315
_04110
OKGIIK_04115: OKGIIK_04115 - Mobile element protein, at 902,363 to 902,650
_04115
OKGIIK_04120: OKGIIK_04120 - DNA-binding protein, at 902,981 to 903,340
_04120
OKGIIK_04130: OKGIIK_04130 - hypothetical protein, at 903,860 to 904,588
_04130
Position (kb) 902
903
904 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 902.682 kb on + strand at 902.682 kb on + strand at 902.682 kb on + strand at 902.682 kb on + strand at 902.682 kb on + strand at 902.682 kb on + strand at 902.682 kb on + strand at 902.705 kb on + strand at 902.705 kb on + strand at 902.748 kb on + strand at 902.748 kb on + strand at 902.748 kb on + strand at 902.748 kb on + strand at 902.748 kb on + strand at 902.749 kb on - strand at 902.749 kb on - strand at 902.749 kb on - strand at 902.749 kb on - strand at 902.749 kb on - strand at 902.791 kb on - strand at 902.791 kb on - strand at 902.982 kb on + strand at 902.983 kb on - strand at 902.983 kb on - strand at 903.237 kb on + strand, within OKGIIK_04120 at 903.237 kb on + strand, within OKGIIK_04120 at 903.238 kb on - strand, within OKGIIK_04120 at 903.328 kb on - strand at 903.328 kb on - strand at 903.394 kb on + strand at 903.394 kb on + strand at 903.394 kb on + strand at 903.394 kb on + strand at 903.394 kb on + strand at 903.394 kb on + strand at 903.394 kb on + strand at 903.394 kb on + strand at 903.461 kb on + strand at 903.463 kb on + strand at 903.463 kb on + strand at 903.463 kb on + strand at 903.749 kb on + strand at 903.749 kb on + strand at 903.749 kb on + strand at 903.749 kb on + strand at 903.749 kb on + strand at 903.749 kb on + strand at 903.749 kb on + strand at 903.749 kb on + strand at 903.750 kb on - strand at 903.750 kb on - strand at 903.750 kb on - strand at 903.750 kb on - strand at 903.750 kb on - strand at 903.750 kb on - strand at 903.750 kb on - strand at 903.750 kb on - strand at 903.750 kb on - strand at 903.750 kb on - strand at 903.750 kb on - strand at 903.750 kb on - strand at 903.750 kb on - strand at 903.769 kb on + strand at 903.770 kb on - strand at 903.770 kb on - strand at 903.795 kb on + strand at 903.848 kb on + strand at 903.848 kb on + strand at 903.848 kb on + strand at 903.848 kb on + strand at 903.848 kb on + strand at 903.848 kb on + strand at 903.848 kb on + strand at 903.848 kb on + strand at 903.848 kb on + strand at 903.849 kb on - strand at 903.849 kb on - strand at 903.849 kb on - strand at 903.849 kb on - strand at 903.849 kb on - strand at 903.899 kb on + strand at 903.899 kb on + strand at 903.899 kb on + strand at 903.899 kb on + strand at 903.899 kb on + strand at 903.900 kb on - strand at 903.900 kb on - strand at 903.900 kb on - strand at 903.900 kb on - strand at 903.900 kb on - strand at 903.900 kb on - strand at 903.947 kb on + strand, within OKGIIK_04130 at 903.947 kb on + strand, within OKGIIK_04130 at 903.947 kb on + strand, within OKGIIK_04130 at 903.947 kb on + strand, within OKGIIK_04130 at 903.947 kb on + strand, within OKGIIK_04130 at 903.947 kb on + strand, within OKGIIK_04130 at 903.947 kb on + strand, within OKGIIK_04130 at 903.947 kb on + strand, within OKGIIK_04130 at 903.948 kb on - strand, within OKGIIK_04130 at 903.948 kb on - strand, within OKGIIK_04130 at 903.948 kb on - strand, within OKGIIK_04130 at 903.948 kb on - strand, within OKGIIK_04130 at 903.948 kb on - strand, within OKGIIK_04130 at 903.948 kb on - strand, within OKGIIK_04130 at 903.948 kb on - strand, within OKGIIK_04130 at 903.983 kb on - strand, within OKGIIK_04130 at 904.161 kb on + strand, within OKGIIK_04130 at 904.184 kb on + strand, within OKGIIK_04130 at 904.184 kb on + strand, within OKGIIK_04130 at 904.184 kb on + strand, within OKGIIK_04130 at 904.184 kb on + strand, within OKGIIK_04130 at 904.184 kb on + strand, within OKGIIK_04130 at 904.184 kb on + strand, within OKGIIK_04130 at 904.184 kb on + strand, within OKGIIK_04130 at 904.184 kb on + strand, within OKGIIK_04130 at 904.184 kb on + strand, within OKGIIK_04130 at 904.184 kb on + strand, within OKGIIK_04130 at 904.185 kb on - strand, within OKGIIK_04130 at 904.185 kb on - strand, within OKGIIK_04130 at 904.185 kb on - strand, within OKGIIK_04130 at 904.185 kb on - strand, within OKGIIK_04130 at 904.216 kb on + strand, within OKGIIK_04130
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Cadmium chloride 128 uM remove 902,682 + +0.1 902,682 + -1.2 902,682 + +0.5 902,682 + -0.3 902,682 + -1.2 902,682 + -0.6 902,682 + -0.5 902,705 + +0.9 902,705 + -0.4 902,748 + -1.7 902,748 + -0.5 902,748 + -1.2 902,748 + +0.3 902,748 + +2.2 902,749 - -0.1 902,749 - +1.2 902,749 - -2.9 902,749 - -0.1 902,749 - +0.2 902,791 - +0.4 902,791 - +0.2 902,982 + -0.7 902,983 - -0.8 902,983 - -0.7 903,237 + OKGIIK_04120 0.71 -0.3 903,237 + OKGIIK_04120 0.71 -1.4 903,238 - OKGIIK_04120 0.71 -1.1 903,328 - +0.2 903,328 - -1.1 903,394 + +0.4 903,394 + -0.4 903,394 + -2.1 903,394 + +0.4 903,394 + -1.1 903,394 + -0.6 903,394 + +1.9 903,394 + +0.4 903,461 + +0.1 903,463 + -0.1 903,463 + +0.2 903,463 + -0.6 903,749 + -0.6 903,749 + -0.6 903,749 + +0.5 903,749 + +0.7 903,749 + -0.3 903,749 + -0.4 903,749 + -0.6 903,749 + +1.1 903,750 - -0.1 903,750 - +1.2 903,750 - -0.1 903,750 - -0.5 903,750 - -2.4 903,750 - -0.8 903,750 - +0.1 903,750 - +0.6 903,750 - -1.0 903,750 - -0.9 903,750 - -0.8 903,750 - -1.3 903,750 - +0.4 903,769 + -1.1 903,770 - -0.8 903,770 - +0.2 903,795 + -0.7 903,848 + -1.7 903,848 + -0.1 903,848 + -0.7 903,848 + -1.2 903,848 + -0.8 903,848 + +1.0 903,848 + -1.3 903,848 + -0.6 903,848 + +0.8 903,849 - -2.4 903,849 - +0.0 903,849 - -0.9 903,849 - +0.1 903,849 - -1.8 903,899 + +1.0 903,899 + +1.0 903,899 + +0.2 903,899 + -0.9 903,899 + -2.0 903,900 - -1.9 903,900 - -0.4 903,900 - -0.7 903,900 - +1.0 903,900 - +0.7 903,900 - -0.4 903,947 + OKGIIK_04130 0.12 +0.1 903,947 + OKGIIK_04130 0.12 -0.4 903,947 + OKGIIK_04130 0.12 -1.1 903,947 + OKGIIK_04130 0.12 +0.1 903,947 + OKGIIK_04130 0.12 +1.9 903,947 + OKGIIK_04130 0.12 -0.1 903,947 + OKGIIK_04130 0.12 -1.9 903,947 + OKGIIK_04130 0.12 +0.2 903,948 - OKGIIK_04130 0.12 -0.2 903,948 - OKGIIK_04130 0.12 -0.7 903,948 - OKGIIK_04130 0.12 -0.4 903,948 - OKGIIK_04130 0.12 -0.1 903,948 - OKGIIK_04130 0.12 +0.6 903,948 - OKGIIK_04130 0.12 -1.5 903,948 - OKGIIK_04130 0.12 -0.6 903,983 - OKGIIK_04130 0.17 -0.9 904,161 + OKGIIK_04130 0.41 -0.8 904,184 + OKGIIK_04130 0.44 -0.8 904,184 + OKGIIK_04130 0.44 -1.6 904,184 + OKGIIK_04130 0.44 -0.6 904,184 + OKGIIK_04130 0.44 -0.9 904,184 + OKGIIK_04130 0.44 +0.2 904,184 + OKGIIK_04130 0.44 +0.9 904,184 + OKGIIK_04130 0.44 -0.4 904,184 + OKGIIK_04130 0.44 -2.9 904,184 + OKGIIK_04130 0.44 -0.6 904,184 + OKGIIK_04130 0.44 -1.0 904,185 - OKGIIK_04130 0.45 +1.1 904,185 - OKGIIK_04130 0.45 +0.6 904,185 - OKGIIK_04130 0.45 -1.0 904,185 - OKGIIK_04130 0.45 -0.9 904,216 + OKGIIK_04130 0.49 +1.5
Or see this region's nucleotide sequence