Experiment: R2A_PIPES with Cadmium chloride 128 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt OKGIIK_01345 and OKGIIK_01350 are separated by 62 nucleotides OKGIIK_01350 and hslO are separated by 86 nucleotides hslO and OKGIIK_01360 are separated by 58 nucleotides
OKGIIK_01345: OKGIIK_01345 - Glycosyl transferase family 2, at 297,932 to 298,870
_01345
OKGIIK_01350: OKGIIK_01350 - Biosynthetic peptidoglycan transglycosylase, at 298,933 to 299,685
_01350
OKGIIK_01355: hslO - heat-shock protein Hsp33, at 299,772 to 300,671
hslO
OKGIIK_01360: OKGIIK_01360 - Cyclic di-GMP metabolism protein, combines GGDEF and EAL domains with a 6TM membrane domain, at 300,730 to 303,627
_01360
Position (kb) 299
300
301 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 298.839 kb on + strand at 298.839 kb on + strand at 298.839 kb on + strand at 298.840 kb on - strand at 298.840 kb on - strand at 298.840 kb on - strand at 298.840 kb on - strand at 298.840 kb on - strand at 298.840 kb on - strand at 298.901 kb on + strand at 298.902 kb on - strand at 298.902 kb on - strand at 298.970 kb on + strand at 298.970 kb on + strand at 298.970 kb on + strand at 298.970 kb on + strand at 298.971 kb on - strand at 298.971 kb on - strand at 298.971 kb on - strand at 298.971 kb on - strand at 298.971 kb on - strand at 298.971 kb on - strand at 298.971 kb on - strand at 298.991 kb on + strand at 298.991 kb on + strand at 298.991 kb on + strand at 298.991 kb on + strand at 298.992 kb on - strand at 298.992 kb on - strand at 298.992 kb on - strand at 298.992 kb on - strand at 299.030 kb on + strand, within OKGIIK_01350 at 299.030 kb on + strand, within OKGIIK_01350 at 299.031 kb on - strand, within OKGIIK_01350 at 299.031 kb on - strand, within OKGIIK_01350 at 299.031 kb on - strand, within OKGIIK_01350 at 299.132 kb on + strand, within OKGIIK_01350 at 299.132 kb on + strand, within OKGIIK_01350 at 299.132 kb on + strand, within OKGIIK_01350 at 299.132 kb on + strand, within OKGIIK_01350 at 299.132 kb on + strand, within OKGIIK_01350 at 299.132 kb on + strand, within OKGIIK_01350 at 299.132 kb on + strand, within OKGIIK_01350 at 299.132 kb on + strand, within OKGIIK_01350 at 299.132 kb on + strand, within OKGIIK_01350 at 299.132 kb on + strand, within OKGIIK_01350 at 299.132 kb on + strand, within OKGIIK_01350 at 299.132 kb on + strand, within OKGIIK_01350 at 299.132 kb on + strand, within OKGIIK_01350 at 299.132 kb on + strand, within OKGIIK_01350 at 299.132 kb on + strand, within OKGIIK_01350 at 299.132 kb on + strand, within OKGIIK_01350 at 299.133 kb on - strand, within OKGIIK_01350 at 299.133 kb on - strand, within OKGIIK_01350 at 299.133 kb on - strand, within OKGIIK_01350 at 299.133 kb on - strand, within OKGIIK_01350 at 299.133 kb on - strand, within OKGIIK_01350 at 299.133 kb on - strand, within OKGIIK_01350 at 299.159 kb on + strand, within OKGIIK_01350 at 299.159 kb on + strand, within OKGIIK_01350 at 299.159 kb on + strand, within OKGIIK_01350 at 299.192 kb on + strand, within OKGIIK_01350 at 299.192 kb on + strand, within OKGIIK_01350 at 299.192 kb on + strand, within OKGIIK_01350 at 299.192 kb on + strand, within OKGIIK_01350 at 299.192 kb on + strand, within OKGIIK_01350 at 299.192 kb on + strand, within OKGIIK_01350 at 299.192 kb on + strand, within OKGIIK_01350 at 299.192 kb on + strand, within OKGIIK_01350 at 299.192 kb on + strand, within OKGIIK_01350 at 299.192 kb on + strand, within OKGIIK_01350 at 299.192 kb on + strand, within OKGIIK_01350 at 299.192 kb on + strand, within OKGIIK_01350 at 299.192 kb on + strand, within OKGIIK_01350 at 299.192 kb on + strand, within OKGIIK_01350 at 299.192 kb on + strand, within OKGIIK_01350 at 299.192 kb on + strand, within OKGIIK_01350 at 299.192 kb on + strand, within OKGIIK_01350 at 299.192 kb on + strand, within OKGIIK_01350 at 299.192 kb on + strand, within OKGIIK_01350 at 299.192 kb on + strand, within OKGIIK_01350 at 299.192 kb on + strand, within OKGIIK_01350 at 299.192 kb on + strand, within OKGIIK_01350 at 299.192 kb on + strand, within OKGIIK_01350 at 299.192 kb on + strand, within OKGIIK_01350 at 299.192 kb on + strand, within OKGIIK_01350 at 299.193 kb on - strand, within OKGIIK_01350 at 299.193 kb on - strand, within OKGIIK_01350 at 299.193 kb on - strand, within OKGIIK_01350 at 299.193 kb on - strand, within OKGIIK_01350 at 299.193 kb on - strand, within OKGIIK_01350 at 299.193 kb on - strand, within OKGIIK_01350 at 299.193 kb on - strand, within OKGIIK_01350 at 299.193 kb on - strand, within OKGIIK_01350 at 299.193 kb on - strand, within OKGIIK_01350 at 299.193 kb on - strand, within OKGIIK_01350 at 299.193 kb on - strand, within OKGIIK_01350 at 299.246 kb on + strand, within OKGIIK_01350 at 299.246 kb on + strand, within OKGIIK_01350 at 299.246 kb on + strand, within OKGIIK_01350 at 299.246 kb on + strand, within OKGIIK_01350 at 299.246 kb on + strand, within OKGIIK_01350 at 299.247 kb on - strand, within OKGIIK_01350 at 299.247 kb on - strand, within OKGIIK_01350 at 299.247 kb on - strand, within OKGIIK_01350 at 299.247 kb on - strand, within OKGIIK_01350 at 299.247 kb on - strand, within OKGIIK_01350 at 299.247 kb on - strand, within OKGIIK_01350 at 299.247 kb on - strand, within OKGIIK_01350 at 299.247 kb on - strand, within OKGIIK_01350 at 299.581 kb on + strand, within OKGIIK_01350 at 299.582 kb on - strand, within OKGIIK_01350 at 299.582 kb on - strand, within OKGIIK_01350 at 299.582 kb on - strand, within OKGIIK_01350 at 299.673 kb on + strand at 299.673 kb on + strand at 299.673 kb on + strand at 299.674 kb on - strand at 299.674 kb on - strand at 299.702 kb on + strand at 299.702 kb on + strand at 299.702 kb on + strand at 299.702 kb on + strand at 299.702 kb on + strand at 299.702 kb on + strand at 299.702 kb on + strand at 299.702 kb on + strand at 299.702 kb on + strand at 299.702 kb on + strand at 299.702 kb on + strand at 299.702 kb on + strand at 299.702 kb on + strand at 299.702 kb on + strand at 299.703 kb on - strand at 299.703 kb on - strand at 299.703 kb on - strand at 299.703 kb on - strand at 299.703 kb on - strand at 299.703 kb on - strand at 299.703 kb on - strand at 299.703 kb on - strand at 299.703 kb on - strand at 299.703 kb on - strand at 299.703 kb on - strand at 299.703 kb on - strand at 299.703 kb on - strand at 299.703 kb on - strand at 299.703 kb on - strand at 299.704 kb on + strand at 299.704 kb on + strand at 299.705 kb on - strand at 299.705 kb on - strand at 299.705 kb on - strand at 299.705 kb on - strand at 299.705 kb on - strand at 300.207 kb on + strand, within hslO at 300.207 kb on + strand, within hslO at 300.207 kb on + strand, within hslO at 300.207 kb on + strand, within hslO at 300.207 kb on + strand, within hslO at 300.208 kb on - strand, within hslO at 300.208 kb on - strand, within hslO at 300.208 kb on - strand, within hslO at 300.208 kb on - strand, within hslO at 300.412 kb on - strand, within hslO at 300.412 kb on - strand, within hslO at 300.420 kb on + strand, within hslO at 300.420 kb on + strand, within hslO at 300.421 kb on - strand, within hslO at 300.421 kb on - strand, within hslO at 300.421 kb on - strand, within hslO at 300.421 kb on - strand, within hslO at 300.421 kb on - strand, within hslO at 300.421 kb on - strand, within hslO at 300.494 kb on - strand, within hslO at 300.594 kb on + strand at 300.594 kb on + strand at 300.595 kb on - strand at 300.595 kb on - strand at 300.595 kb on - strand at 300.595 kb on - strand at 300.669 kb on + strand at 300.670 kb on - strand at 300.670 kb on - strand at 300.670 kb on - strand at 300.670 kb on - strand at 301.007 kb on + strand at 301.007 kb on + strand at 301.007 kb on + strand at 301.007 kb on + strand at 301.007 kb on + strand at 301.007 kb on + strand at 301.007 kb on + strand at 301.007 kb on + strand at 301.007 kb on + strand at 301.008 kb on - strand at 301.008 kb on - strand at 301.008 kb on - strand at 301.008 kb on - strand at 301.008 kb on - strand at 301.008 kb on - strand at 301.022 kb on + strand, within OKGIIK_01360 at 301.022 kb on + strand, within OKGIIK_01360 at 301.022 kb on + strand, within OKGIIK_01360 at 301.022 kb on + strand, within OKGIIK_01360 at 301.022 kb on + strand, within OKGIIK_01360 at 301.160 kb on + strand, within OKGIIK_01360 at 301.161 kb on - strand, within OKGIIK_01360 at 301.161 kb on - strand, within OKGIIK_01360 at 301.161 kb on - strand, within OKGIIK_01360 at 301.161 kb on - strand, within OKGIIK_01360 at 301.161 kb on - strand, within OKGIIK_01360 at 301.439 kb on + strand, within OKGIIK_01360 at 301.439 kb on + strand, within OKGIIK_01360 at 301.439 kb on + strand, within OKGIIK_01360 at 301.439 kb on + strand, within OKGIIK_01360 at 301.440 kb on - strand, within OKGIIK_01360 at 301.440 kb on - strand, within OKGIIK_01360 at 301.440 kb on - strand, within OKGIIK_01360 at 301.571 kb on + strand, within OKGIIK_01360 at 301.571 kb on + strand, within OKGIIK_01360 at 301.571 kb on + strand, within OKGIIK_01360 at 301.571 kb on + strand, within OKGIIK_01360 at 301.571 kb on + strand, within OKGIIK_01360 at 301.571 kb on + strand, within OKGIIK_01360 at 301.571 kb on + strand, within OKGIIK_01360 at 301.571 kb on + strand, within OKGIIK_01360 at 301.571 kb on + strand, within OKGIIK_01360 at 301.571 kb on + strand, within OKGIIK_01360 at 301.571 kb on + strand, within OKGIIK_01360 at 301.571 kb on + strand, within OKGIIK_01360 at 301.571 kb on + strand, within OKGIIK_01360 at 301.571 kb on + strand, within OKGIIK_01360 at 301.571 kb on + strand, within OKGIIK_01360 at 301.571 kb on + strand, within OKGIIK_01360 at 301.572 kb on - strand, within OKGIIK_01360 at 301.572 kb on - strand, within OKGIIK_01360 at 301.572 kb on - strand, within OKGIIK_01360 at 301.572 kb on - strand, within OKGIIK_01360 at 301.616 kb on + strand, within OKGIIK_01360
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Cadmium chloride 128 uM remove 298,839 + +0.5 298,839 + +1.3 298,839 + -0.8 298,840 - +0.1 298,840 - -0.3 298,840 - -1.3 298,840 - -1.5 298,840 - -0.3 298,840 - -0.5 298,901 + +0.4 298,902 - -0.2 298,902 - -0.9 298,970 + -1.1 298,970 + +0.5 298,970 + +0.3 298,970 + -1.4 298,971 - -1.2 298,971 - -1.2 298,971 - +0.1 298,971 - +0.1 298,971 - +0.2 298,971 - +0.9 298,971 - -0.9 298,991 + +0.5 298,991 + -0.5 298,991 + -0.1 298,991 + -0.3 298,992 - -0.3 298,992 - +1.3 298,992 - +0.2 298,992 - +0.6 299,030 + OKGIIK_01350 0.13 +0.4 299,030 + OKGIIK_01350 0.13 -1.3 299,031 - OKGIIK_01350 0.13 +0.1 299,031 - OKGIIK_01350 0.13 -0.7 299,031 - OKGIIK_01350 0.13 +1.1 299,132 + OKGIIK_01350 0.26 +0.8 299,132 + OKGIIK_01350 0.26 -0.9 299,132 + OKGIIK_01350 0.26 +1.1 299,132 + OKGIIK_01350 0.26 +0.1 299,132 + OKGIIK_01350 0.26 -0.6 299,132 + OKGIIK_01350 0.26 +0.1 299,132 + OKGIIK_01350 0.26 -0.3 299,132 + OKGIIK_01350 0.26 +0.1 299,132 + OKGIIK_01350 0.26 -1.5 299,132 + OKGIIK_01350 0.26 +0.5 299,132 + OKGIIK_01350 0.26 -0.6 299,132 + OKGIIK_01350 0.26 -1.5 299,132 + OKGIIK_01350 0.26 +1.5 299,132 + OKGIIK_01350 0.26 +1.3 299,132 + OKGIIK_01350 0.26 -0.0 299,132 + OKGIIK_01350 0.26 +2.9 299,133 - OKGIIK_01350 0.27 -0.6 299,133 - OKGIIK_01350 0.27 -1.6 299,133 - OKGIIK_01350 0.27 -0.3 299,133 - OKGIIK_01350 0.27 +0.6 299,133 - OKGIIK_01350 0.27 +0.2 299,133 - OKGIIK_01350 0.27 +1.7 299,159 + OKGIIK_01350 0.30 +0.1 299,159 + OKGIIK_01350 0.30 +0.6 299,159 + OKGIIK_01350 0.30 -0.2 299,192 + OKGIIK_01350 0.34 -1.5 299,192 + OKGIIK_01350 0.34 -1.6 299,192 + OKGIIK_01350 0.34 +0.0 299,192 + OKGIIK_01350 0.34 +0.5 299,192 + OKGIIK_01350 0.34 +0.7 299,192 + OKGIIK_01350 0.34 +0.3 299,192 + OKGIIK_01350 0.34 -2.1 299,192 + OKGIIK_01350 0.34 +1.2 299,192 + OKGIIK_01350 0.34 -0.3 299,192 + OKGIIK_01350 0.34 -0.3 299,192 + OKGIIK_01350 0.34 -1.3 299,192 + OKGIIK_01350 0.34 -0.5 299,192 + OKGIIK_01350 0.34 +0.3 299,192 + OKGIIK_01350 0.34 -0.7 299,192 + OKGIIK_01350 0.34 -0.5 299,192 + OKGIIK_01350 0.34 -0.3 299,192 + OKGIIK_01350 0.34 -1.1 299,192 + OKGIIK_01350 0.34 -1.4 299,192 + OKGIIK_01350 0.34 -1.7 299,192 + OKGIIK_01350 0.34 -0.4 299,192 + OKGIIK_01350 0.34 +0.4 299,192 + OKGIIK_01350 0.34 -0.0 299,192 + OKGIIK_01350 0.34 +0.1 299,192 + OKGIIK_01350 0.34 +1.3 299,192 + OKGIIK_01350 0.34 +0.8 299,193 - OKGIIK_01350 0.35 -0.3 299,193 - OKGIIK_01350 0.35 +1.3 299,193 - OKGIIK_01350 0.35 -2.3 299,193 - OKGIIK_01350 0.35 -0.5 299,193 - OKGIIK_01350 0.35 -0.6 299,193 - OKGIIK_01350 0.35 -1.8 299,193 - OKGIIK_01350 0.35 +0.1 299,193 - OKGIIK_01350 0.35 -1.0 299,193 - OKGIIK_01350 0.35 -2.0 299,193 - OKGIIK_01350 0.35 +1.8 299,193 - OKGIIK_01350 0.35 +1.2 299,246 + OKGIIK_01350 0.42 -0.3 299,246 + OKGIIK_01350 0.42 -0.7 299,246 + OKGIIK_01350 0.42 -1.5 299,246 + OKGIIK_01350 0.42 -0.0 299,246 + OKGIIK_01350 0.42 -1.6 299,247 - OKGIIK_01350 0.42 +0.7 299,247 - OKGIIK_01350 0.42 -1.3 299,247 - OKGIIK_01350 0.42 -1.9 299,247 - OKGIIK_01350 0.42 +1.1 299,247 - OKGIIK_01350 0.42 +0.1 299,247 - OKGIIK_01350 0.42 +0.3 299,247 - OKGIIK_01350 0.42 -1.9 299,247 - OKGIIK_01350 0.42 -1.2 299,581 + OKGIIK_01350 0.86 -1.1 299,582 - OKGIIK_01350 0.86 +0.1 299,582 - OKGIIK_01350 0.86 +0.8 299,582 - OKGIIK_01350 0.86 -1.8 299,673 + +0.5 299,673 + -1.1 299,673 + -1.6 299,674 - -0.5 299,674 - -0.4 299,702 + +0.7 299,702 + +0.6 299,702 + +1.4 299,702 + -1.3 299,702 + +0.1 299,702 + +1.2 299,702 + +0.1 299,702 + -0.5 299,702 + -1.2 299,702 + -1.4 299,702 + -0.1 299,702 + -0.3 299,702 + +0.3 299,702 + -0.9 299,703 - +0.4 299,703 - +0.7 299,703 - -1.1 299,703 - -0.3 299,703 - -0.5 299,703 - -0.7 299,703 - -0.4 299,703 - -0.3 299,703 - +0.7 299,703 - +1.1 299,703 - -1.8 299,703 - -0.6 299,703 - -0.7 299,703 - +0.1 299,703 - -0.6 299,704 + +2.3 299,704 + -0.1 299,705 - -1.3 299,705 - -0.5 299,705 - -1.1 299,705 - -0.8 299,705 - -0.0 300,207 + hslO OKGIIK_01355 0.48 -0.3 300,207 + hslO OKGIIK_01355 0.48 -0.1 300,207 + hslO OKGIIK_01355 0.48 -0.5 300,207 + hslO OKGIIK_01355 0.48 +0.5 300,207 + hslO OKGIIK_01355 0.48 +1.1 300,208 - hslO OKGIIK_01355 0.48 -0.0 300,208 - hslO OKGIIK_01355 0.48 +0.3 300,208 - hslO OKGIIK_01355 0.48 +2.1 300,208 - hslO OKGIIK_01355 0.48 +0.1 300,412 - hslO OKGIIK_01355 0.71 -0.1 300,412 - hslO OKGIIK_01355 0.71 +1.7 300,420 + hslO OKGIIK_01355 0.72 +0.1 300,420 + hslO OKGIIK_01355 0.72 -1.2 300,421 - hslO OKGIIK_01355 0.72 +1.5 300,421 - hslO OKGIIK_01355 0.72 -0.7 300,421 - hslO OKGIIK_01355 0.72 -0.3 300,421 - hslO OKGIIK_01355 0.72 -1.7 300,421 - hslO OKGIIK_01355 0.72 -1.7 300,421 - hslO OKGIIK_01355 0.72 +0.5 300,494 - hslO OKGIIK_01355 0.80 -0.4 300,594 + -0.6 300,594 + -1.1 300,595 - -1.1 300,595 - +0.5 300,595 - +0.9 300,595 - -1.3 300,669 + -0.8 300,670 - +1.1 300,670 - +0.5 300,670 - -0.4 300,670 - +0.1 301,007 + +0.0 301,007 + +0.3 301,007 + +1.6 301,007 + -0.9 301,007 + -0.2 301,007 + -0.2 301,007 + -1.7 301,007 + -1.1 301,007 + -0.3 301,008 - -0.1 301,008 - -1.1 301,008 - -0.7 301,008 - -1.1 301,008 - -1.1 301,008 - -0.3 301,022 + OKGIIK_01360 0.10 +1.1 301,022 + OKGIIK_01360 0.10 -1.9 301,022 + OKGIIK_01360 0.10 -0.5 301,022 + OKGIIK_01360 0.10 +0.7 301,022 + OKGIIK_01360 0.10 +0.1 301,160 + OKGIIK_01360 0.15 +0.9 301,161 - OKGIIK_01360 0.15 -1.8 301,161 - OKGIIK_01360 0.15 -1.4 301,161 - OKGIIK_01360 0.15 -0.1 301,161 - OKGIIK_01360 0.15 +1.3 301,161 - OKGIIK_01360 0.15 -0.1 301,439 + OKGIIK_01360 0.24 -1.1 301,439 + OKGIIK_01360 0.24 +0.1 301,439 + OKGIIK_01360 0.24 +0.8 301,439 + OKGIIK_01360 0.24 +0.1 301,440 - OKGIIK_01360 0.24 -0.5 301,440 - OKGIIK_01360 0.24 +1.1 301,440 - OKGIIK_01360 0.24 -0.3 301,571 + OKGIIK_01360 0.29 -1.6 301,571 + OKGIIK_01360 0.29 +0.4 301,571 + OKGIIK_01360 0.29 -1.4 301,571 + OKGIIK_01360 0.29 -0.9 301,571 + OKGIIK_01360 0.29 +0.1 301,571 + OKGIIK_01360 0.29 -0.4 301,571 + OKGIIK_01360 0.29 +0.3 301,571 + OKGIIK_01360 0.29 -2.3 301,571 + OKGIIK_01360 0.29 +1.2 301,571 + OKGIIK_01360 0.29 +0.2 301,571 + OKGIIK_01360 0.29 -1.3 301,571 + OKGIIK_01360 0.29 -1.1 301,571 + OKGIIK_01360 0.29 -0.6 301,571 + OKGIIK_01360 0.29 -0.7 301,571 + OKGIIK_01360 0.29 +2.1 301,571 + OKGIIK_01360 0.29 -0.3 301,572 - OKGIIK_01360 0.29 -0.9 301,572 - OKGIIK_01360 0.29 +0.1 301,572 - OKGIIK_01360 0.29 +0.8 301,572 - OKGIIK_01360 0.29 -0.3 301,616 + OKGIIK_01360 0.31 -0.3
Or see this region's nucleotide sequence