Experiment: R2A_PIPES with Cadmium chloride 128 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt gcvT and gcvH are separated by 91 nucleotides gcvH and OKGIIK_01315 are separated by 897 nucleotides
OKGIIK_01305: gcvT - glycine cleavage system aminomethyltransferase GcvT, at 288,586 to 289,719
gcvT
OKGIIK_01310: gcvH - glycine cleavage system protein GcvH, at 289,811 to 290,206
gcvH
OKGIIK_01315: OKGIIK_01315 - Histone-like protein 2, at 291,104 to 291,394
_01315
Position (kb) 289
290
291 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 288.872 kb on - strand, within gcvT at 288.922 kb on + strand, within gcvT at 288.923 kb on - strand, within gcvT at 288.923 kb on - strand, within gcvT at 289.198 kb on + strand, within gcvT at 289.199 kb on - strand, within gcvT at 289.679 kb on - strand at 289.681 kb on - strand at 289.762 kb on + strand at 289.762 kb on + strand at 289.762 kb on + strand at 289.762 kb on + strand at 289.762 kb on + strand at 289.762 kb on + strand at 289.762 kb on + strand at 289.762 kb on + strand at 289.763 kb on - strand at 289.763 kb on - strand at 289.763 kb on - strand at 289.777 kb on + strand at 289.934 kb on + strand, within gcvH at 289.935 kb on - strand, within gcvH at 290.021 kb on + strand, within gcvH at 290.021 kb on + strand, within gcvH at 290.021 kb on + strand, within gcvH at 290.175 kb on - strand at 290.175 kb on - strand at 290.385 kb on + strand at 290.385 kb on + strand at 290.385 kb on + strand at 290.385 kb on + strand at 290.385 kb on + strand at 290.385 kb on + strand at 290.385 kb on + strand at 290.385 kb on + strand at 290.385 kb on + strand at 290.385 kb on + strand at 290.385 kb on + strand at 290.385 kb on + strand at 290.385 kb on + strand at 290.385 kb on + strand at 290.385 kb on + strand at 290.386 kb on - strand at 290.386 kb on - strand at 290.386 kb on - strand at 290.386 kb on - strand at 290.690 kb on + strand at 290.702 kb on + strand at 290.718 kb on + strand at 290.836 kb on - strand at 290.869 kb on + strand at 290.897 kb on + strand at 290.897 kb on + strand at 290.898 kb on - strand at 290.898 kb on - strand at 290.928 kb on + strand at 290.929 kb on - strand at 290.982 kb on + strand at 290.982 kb on + strand at 290.983 kb on - strand at 290.983 kb on - strand at 290.983 kb on - strand at 290.986 kb on + strand at 291.019 kb on + strand at 291.062 kb on - strand at 291.094 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Cadmium chloride 128 uM remove 288,872 - gcvT OKGIIK_01305 0.25 +0.5 288,922 + gcvT OKGIIK_01305 0.30 -0.8 288,923 - gcvT OKGIIK_01305 0.30 -1.3 288,923 - gcvT OKGIIK_01305 0.30 -0.5 289,198 + gcvT OKGIIK_01305 0.54 +0.8 289,199 - gcvT OKGIIK_01305 0.54 +0.5 289,679 - +0.3 289,681 - +1.7 289,762 + -0.7 289,762 + -1.3 289,762 + -1.3 289,762 + -0.0 289,762 + -0.8 289,762 + -0.0 289,762 + -0.8 289,762 + -0.2 289,763 - +0.8 289,763 - +1.3 289,763 - -0.7 289,777 + -0.8 289,934 + gcvH OKGIIK_01310 0.31 -1.1 289,935 - gcvH OKGIIK_01310 0.31 -1.0 290,021 + gcvH OKGIIK_01310 0.53 -2.0 290,021 + gcvH OKGIIK_01310 0.53 -1.5 290,021 + gcvH OKGIIK_01310 0.53 -2.9 290,175 - +0.5 290,175 - -2.2 290,385 + -0.9 290,385 + -2.3 290,385 + +1.0 290,385 + +1.5 290,385 + -3.0 290,385 + -0.9 290,385 + -0.9 290,385 + +1.3 290,385 + -0.5 290,385 + +0.0 290,385 + -0.5 290,385 + -1.0 290,385 + -0.3 290,385 + +0.6 290,385 + -1.8 290,386 - +0.1 290,386 - +0.0 290,386 - -1.1 290,386 - +0.6 290,690 + +1.2 290,702 + +0.7 290,718 + -0.9 290,836 - +0.1 290,869 + +1.5 290,897 + +0.0 290,897 + -0.4 290,898 - -0.5 290,898 - -0.1 290,928 + +0.2 290,929 - -1.1 290,982 + -1.8 290,982 + -0.5 290,983 - -1.4 290,983 - +1.0 290,983 - -1.0 290,986 + +1.4 291,019 + -0.3 291,062 - -0.4 291,094 - +1.0
Or see this region's nucleotide sequence