Experiment: R2A_PIPES with Cadmium chloride 128 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt OKGIIK_01100 and gph are separated by 105 nucleotides gph and pgk overlap by 1 nucleotides pgk and maiA are separated by 178 nucleotides maiA and pilU are separated by 92 nucleotides
OKGIIK_01100: OKGIIK_01100 - Transketolase 1, at 237,522 to 237,785
_01100
OKGIIK_01105: gph - HAD family hydrolase, at 237,891 to 238,529
gph
OKGIIK_01110: pgk - phosphoglycerate kinase, at 238,529 to 239,713
pgk
OKGIIK_01115: maiA - maleylacetoacetate isomerase, at 239,892 to 240,566
maiA
OKGIIK_01120: pilU - Type IV pilus assembly protein, ATPase PilU, at 240,659 to 241,771
pilU
Position (kb) 238
239
240 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 237.577 kb on - strand, within OKGIIK_01100 at 237.804 kb on - strand at 237.870 kb on + strand at 237.927 kb on - strand at 238.144 kb on + strand, within gph at 238.145 kb on - strand, within gph at 238.145 kb on - strand, within gph at 238.145 kb on - strand, within gph at 238.279 kb on + strand, within gph at 238.279 kb on + strand, within gph at 238.279 kb on + strand, within gph at 238.279 kb on + strand, within gph at 238.280 kb on - strand, within gph at 238.280 kb on - strand, within gph at 238.280 kb on - strand, within gph at 238.280 kb on - strand, within gph at 238.280 kb on - strand, within gph at 238.280 kb on - strand, within gph at 238.324 kb on + strand, within gph at 238.324 kb on + strand, within gph at 238.324 kb on + strand, within gph at 238.324 kb on + strand, within gph at 238.324 kb on + strand, within gph at 238.324 kb on + strand, within gph at 238.325 kb on - strand, within gph at 238.325 kb on - strand, within gph at 238.325 kb on - strand, within gph at 238.325 kb on - strand, within gph at 238.325 kb on - strand, within gph at 238.325 kb on - strand, within gph at 238.327 kb on + strand, within gph at 238.327 kb on + strand, within gph at 238.327 kb on + strand, within gph at 238.327 kb on + strand, within gph at 238.327 kb on + strand, within gph at 238.327 kb on + strand, within gph at 238.328 kb on - strand, within gph at 238.328 kb on - strand, within gph at 238.328 kb on - strand, within gph at 238.328 kb on - strand, within gph at 238.530 kb on + strand at 238.530 kb on + strand at 238.530 kb on + strand at 238.530 kb on + strand at 238.530 kb on + strand at 238.530 kb on + strand at 238.530 kb on + strand at 238.530 kb on + strand at 238.530 kb on + strand at 238.530 kb on + strand at 238.530 kb on + strand at 238.530 kb on + strand at 238.530 kb on + strand at 238.530 kb on + strand at 238.530 kb on + strand at 238.531 kb on - strand at 238.531 kb on - strand at 238.531 kb on - strand at 238.531 kb on - strand at 238.531 kb on - strand at 238.531 kb on - strand at 238.531 kb on - strand at 238.531 kb on - strand at 238.531 kb on - strand at 238.531 kb on - strand at 238.531 kb on - strand at 238.531 kb on - strand at 238.531 kb on - strand at 238.531 kb on - strand at 238.531 kb on - strand at 238.531 kb on - strand at 238.531 kb on - strand at 238.531 kb on - strand at 238.531 kb on - strand at 238.531 kb on - strand at 238.531 kb on - strand at 238.531 kb on - strand at 238.531 kb on - strand at 238.531 kb on - strand at 238.531 kb on - strand at 238.620 kb on + strand at 238.620 kb on + strand at 238.620 kb on + strand at 238.620 kb on + strand at 238.620 kb on + strand at 238.620 kb on + strand at 238.620 kb on + strand at 238.620 kb on + strand at 238.620 kb on + strand at 238.621 kb on - strand at 238.621 kb on - strand at 238.621 kb on - strand at 238.621 kb on - strand at 238.621 kb on - strand at 238.621 kb on - strand at 238.621 kb on - strand at 238.621 kb on - strand at 238.644 kb on + strand at 238.644 kb on + strand at 238.644 kb on + strand at 238.644 kb on + strand at 238.645 kb on - strand at 238.812 kb on + strand, within pgk at 239.238 kb on + strand, within pgk at 239.238 kb on + strand, within pgk at 239.238 kb on + strand, within pgk at 239.238 kb on + strand, within pgk at 239.238 kb on + strand, within pgk at 239.238 kb on + strand, within pgk at 239.239 kb on - strand, within pgk at 239.239 kb on - strand, within pgk at 239.239 kb on - strand, within pgk at 239.239 kb on - strand, within pgk at 239.239 kb on - strand, within pgk at 239.239 kb on - strand, within pgk at 239.239 kb on - strand, within pgk at 239.239 kb on - strand, within pgk at 239.322 kb on + strand, within pgk at 239.322 kb on + strand, within pgk at 239.323 kb on - strand, within pgk at 239.323 kb on - strand, within pgk at 239.323 kb on - strand, within pgk at 239.427 kb on + strand, within pgk at 239.428 kb on - strand, within pgk at 239.428 kb on - strand, within pgk at 239.444 kb on + strand, within pgk at 239.445 kb on - strand, within pgk at 239.767 kb on - strand at 239.767 kb on - strand at 239.767 kb on - strand at 239.767 kb on - strand at 239.767 kb on - strand at 239.767 kb on - strand at 239.851 kb on + strand at 239.852 kb on - strand at 239.852 kb on - strand at 239.852 kb on - strand at 239.852 kb on - strand at 239.852 kb on - strand at 239.852 kb on - strand at 239.891 kb on + strand at 239.891 kb on + strand at 239.891 kb on + strand at 239.891 kb on + strand at 239.891 kb on + strand at 239.892 kb on - strand at 239.892 kb on - strand at 239.892 kb on - strand at 239.892 kb on - strand at 239.892 kb on - strand at 239.892 kb on - strand at 239.892 kb on - strand at 239.892 kb on - strand at 239.892 kb on - strand at 239.892 kb on - strand at 239.913 kb on + strand at 239.913 kb on + strand at 239.930 kb on + strand at 239.930 kb on + strand at 239.930 kb on + strand at 239.930 kb on + strand at 239.930 kb on + strand at 239.930 kb on + strand at 239.931 kb on - strand at 239.931 kb on - strand at 239.931 kb on - strand at 239.951 kb on + strand at 239.952 kb on - strand at 239.952 kb on - strand at 239.980 kb on - strand, within maiA at 239.980 kb on - strand, within maiA at 239.980 kb on - strand, within maiA at 240.120 kb on + strand, within maiA at 240.120 kb on + strand, within maiA at 240.120 kb on + strand, within maiA at 240.121 kb on - strand, within maiA at 240.121 kb on - strand, within maiA at 240.121 kb on - strand, within maiA at 240.121 kb on - strand, within maiA at 240.121 kb on - strand, within maiA at 240.375 kb on + strand, within maiA at 240.376 kb on - strand, within maiA at 240.429 kb on + strand, within maiA at 240.429 kb on + strand, within maiA at 240.429 kb on + strand, within maiA at 240.429 kb on + strand, within maiA at 240.429 kb on + strand, within maiA at 240.429 kb on + strand, within maiA at 240.429 kb on + strand, within maiA at 240.429 kb on + strand, within maiA at 240.429 kb on + strand, within maiA at 240.430 kb on - strand, within maiA at 240.430 kb on - strand, within maiA at 240.430 kb on - strand, within maiA at 240.430 kb on - strand, within maiA at 240.430 kb on - strand, within maiA
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Cadmium chloride 128 uM remove 237,577 - OKGIIK_01100 0.21 -1.4 237,804 - -0.4 237,870 + +0.0 237,927 - -2.0 238,144 + gph OKGIIK_01105 0.40 +0.0 238,145 - gph OKGIIK_01105 0.40 -1.1 238,145 - gph OKGIIK_01105 0.40 -1.2 238,145 - gph OKGIIK_01105 0.40 +0.8 238,279 + gph OKGIIK_01105 0.61 +0.1 238,279 + gph OKGIIK_01105 0.61 -1.3 238,279 + gph OKGIIK_01105 0.61 -2.0 238,279 + gph OKGIIK_01105 0.61 -0.3 238,280 - gph OKGIIK_01105 0.61 -0.2 238,280 - gph OKGIIK_01105 0.61 -0.5 238,280 - gph OKGIIK_01105 0.61 -0.0 238,280 - gph OKGIIK_01105 0.61 -2.1 238,280 - gph OKGIIK_01105 0.61 -0.1 238,280 - gph OKGIIK_01105 0.61 +1.2 238,324 + gph OKGIIK_01105 0.68 -0.5 238,324 + gph OKGIIK_01105 0.68 +0.0 238,324 + gph OKGIIK_01105 0.68 -0.5 238,324 + gph OKGIIK_01105 0.68 -1.5 238,324 + gph OKGIIK_01105 0.68 +0.0 238,324 + gph OKGIIK_01105 0.68 +0.5 238,325 - gph OKGIIK_01105 0.68 +1.0 238,325 - gph OKGIIK_01105 0.68 -1.0 238,325 - gph OKGIIK_01105 0.68 -1.2 238,325 - gph OKGIIK_01105 0.68 -0.8 238,325 - gph OKGIIK_01105 0.68 +0.4 238,325 - gph OKGIIK_01105 0.68 +2.6 238,327 + gph OKGIIK_01105 0.68 +0.2 238,327 + gph OKGIIK_01105 0.68 -1.9 238,327 + gph OKGIIK_01105 0.68 +0.3 238,327 + gph OKGIIK_01105 0.68 -0.3 238,327 + gph OKGIIK_01105 0.68 +0.0 238,327 + gph OKGIIK_01105 0.68 +0.2 238,328 - gph OKGIIK_01105 0.68 +0.8 238,328 - gph OKGIIK_01105 0.68 -0.5 238,328 - gph OKGIIK_01105 0.68 +1.4 238,328 - gph OKGIIK_01105 0.68 -1.9 238,530 + +0.2 238,530 + +0.0 238,530 + +0.9 238,530 + +0.7 238,530 + +0.7 238,530 + -0.8 238,530 + -1.9 238,530 + +0.7 238,530 + -0.3 238,530 + +1.0 238,530 + +0.0 238,530 + -2.5 238,530 + -1.7 238,530 + -0.7 238,530 + +0.8 238,531 - -0.5 238,531 - -0.3 238,531 - -0.5 238,531 - +1.2 238,531 - -1.4 238,531 - +0.6 238,531 - -0.0 238,531 - +0.0 238,531 - +0.5 238,531 - -0.2 238,531 - -1.7 238,531 - -1.1 238,531 - +0.3 238,531 - -0.8 238,531 - +0.2 238,531 - -1.0 238,531 - +0.0 238,531 - +0.0 238,531 - -0.8 238,531 - +0.3 238,531 - -1.0 238,531 - -1.7 238,531 - -1.0 238,531 - +0.3 238,531 - +0.0 238,620 + +0.0 238,620 + -0.2 238,620 + +1.3 238,620 + -1.0 238,620 + +0.2 238,620 + -1.3 238,620 + -0.1 238,620 + -1.3 238,620 + -0.3 238,621 - +0.0 238,621 - -0.3 238,621 - -0.3 238,621 - +0.2 238,621 - -1.8 238,621 - -0.3 238,621 - +0.0 238,621 - -0.3 238,644 + -0.8 238,644 + -0.5 238,644 + -0.5 238,644 + -1.0 238,645 - -0.8 238,812 + pgk OKGIIK_01110 0.24 -1.6 239,238 + pgk OKGIIK_01110 0.60 -0.3 239,238 + pgk OKGIIK_01110 0.60 -1.0 239,238 + pgk OKGIIK_01110 0.60 -0.2 239,238 + pgk OKGIIK_01110 0.60 -0.5 239,238 + pgk OKGIIK_01110 0.60 +0.0 239,238 + pgk OKGIIK_01110 0.60 -0.3 239,239 - pgk OKGIIK_01110 0.60 -0.8 239,239 - pgk OKGIIK_01110 0.60 +0.0 239,239 - pgk OKGIIK_01110 0.60 -1.3 239,239 - pgk OKGIIK_01110 0.60 +0.1 239,239 - pgk OKGIIK_01110 0.60 -0.3 239,239 - pgk OKGIIK_01110 0.60 -0.1 239,239 - pgk OKGIIK_01110 0.60 +0.0 239,239 - pgk OKGIIK_01110 0.60 +1.0 239,322 + pgk OKGIIK_01110 0.67 +0.5 239,322 + pgk OKGIIK_01110 0.67 -0.5 239,323 - pgk OKGIIK_01110 0.67 -2.1 239,323 - pgk OKGIIK_01110 0.67 -2.3 239,323 - pgk OKGIIK_01110 0.67 +0.5 239,427 + pgk OKGIIK_01110 0.76 -0.5 239,428 - pgk OKGIIK_01110 0.76 +0.0 239,428 - pgk OKGIIK_01110 0.76 -0.3 239,444 + pgk OKGIIK_01110 0.77 -0.2 239,445 - pgk OKGIIK_01110 0.77 +0.0 239,767 - -0.3 239,767 - +0.5 239,767 - -2.0 239,767 - +0.8 239,767 - +0.2 239,767 - -0.6 239,851 + +2.0 239,852 - -1.5 239,852 - -1.3 239,852 - +0.0 239,852 - +0.7 239,852 - -1.5 239,852 - +1.4 239,891 + +1.1 239,891 + -1.1 239,891 + -1.0 239,891 + -2.3 239,891 + -0.4 239,892 - -1.0 239,892 - -1.1 239,892 - -1.3 239,892 - +0.5 239,892 - -0.3 239,892 - +0.0 239,892 - -0.5 239,892 - +0.6 239,892 - +0.9 239,892 - +0.0 239,913 + +0.7 239,913 + +0.2 239,930 + +1.7 239,930 + +0.7 239,930 + +0.7 239,930 + -1.2 239,930 + -0.9 239,930 + +0.6 239,931 - -1.1 239,931 - -1.7 239,931 - -1.9 239,951 + +0.3 239,952 - -1.7 239,952 - +0.0 239,980 - maiA OKGIIK_01115 0.13 -1.1 239,980 - maiA OKGIIK_01115 0.13 -0.4 239,980 - maiA OKGIIK_01115 0.13 +0.0 240,120 + maiA OKGIIK_01115 0.34 -1.0 240,120 + maiA OKGIIK_01115 0.34 -0.7 240,120 + maiA OKGIIK_01115 0.34 +0.3 240,121 - maiA OKGIIK_01115 0.34 -1.4 240,121 - maiA OKGIIK_01115 0.34 -2.9 240,121 - maiA OKGIIK_01115 0.34 +0.5 240,121 - maiA OKGIIK_01115 0.34 +0.9 240,121 - maiA OKGIIK_01115 0.34 +0.0 240,375 + maiA OKGIIK_01115 0.72 -0.3 240,376 - maiA OKGIIK_01115 0.72 -1.0 240,429 + maiA OKGIIK_01115 0.80 -0.5 240,429 + maiA OKGIIK_01115 0.80 -1.1 240,429 + maiA OKGIIK_01115 0.80 +0.6 240,429 + maiA OKGIIK_01115 0.80 -0.4 240,429 + maiA OKGIIK_01115 0.80 -0.1 240,429 + maiA OKGIIK_01115 0.80 -0.1 240,429 + maiA OKGIIK_01115 0.80 -0.6 240,429 + maiA OKGIIK_01115 0.80 -1.1 240,429 + maiA OKGIIK_01115 0.80 -1.0 240,430 - maiA OKGIIK_01115 0.80 +0.4 240,430 - maiA OKGIIK_01115 0.80 +0.5 240,430 - maiA OKGIIK_01115 0.80 +0.7 240,430 - maiA OKGIIK_01115 0.80 +0.8 240,430 - maiA OKGIIK_01115 0.80 +0.0
Or see this region's nucleotide sequence