Experiment: Mixed culture; Methylosinus sp. DMC45 1:1 with Pseudomonas_DMC3_ML3; dNMS with methane
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3858 and GFF3859 are separated by 302 nucleotides GFF3859 and GFF3860 are separated by 198 nucleotides
GFF3858 - Aldehyde dehydrogenase, at 105,983 to 107,503
GFF3858
GFF3859 - Acetoin catabolism regulatory protein, at 107,806 to 109,716
GFF3859
GFF3860 - UDP-N-acetylmuramate--L-alanyl-gamma-D-glutamyl-m eso-2,6-diaminoheptandioate ligase, at 109,915 to 111,264
GFF3860
Position (kb)
107
108
109
110 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 107.031 kb on - strand, within GFF3858 at 107.031 kb on - strand, within GFF3858 at 107.031 kb on - strand, within GFF3858 at 107.058 kb on + strand, within GFF3858 at 107.059 kb on - strand, within GFF3858 at 107.059 kb on - strand, within GFF3858 at 107.059 kb on - strand, within GFF3858 at 107.389 kb on + strand at 107.400 kb on + strand at 107.400 kb on + strand at 107.400 kb on + strand at 107.400 kb on + strand at 107.401 kb on - strand at 107.401 kb on - strand at 107.439 kb on + strand at 107.439 kb on + strand at 107.439 kb on + strand at 107.440 kb on - strand at 107.440 kb on - strand at 107.440 kb on - strand at 107.440 kb on - strand at 107.440 kb on - strand at 107.440 kb on - strand at 107.440 kb on - strand at 107.448 kb on + strand at 107.449 kb on - strand at 107.449 kb on - strand at 107.526 kb on + strand at 107.526 kb on + strand at 107.526 kb on + strand at 107.527 kb on - strand at 107.527 kb on - strand at 107.527 kb on - strand at 107.530 kb on + strand at 107.530 kb on + strand at 107.530 kb on + strand at 107.534 kb on - strand at 107.560 kb on + strand at 107.566 kb on + strand at 107.684 kb on - strand at 107.800 kb on + strand at 107.801 kb on - strand at 107.801 kb on - strand at 107.801 kb on - strand at 107.801 kb on - strand at 107.932 kb on + strand at 107.933 kb on - strand at 107.933 kb on - strand at 108.216 kb on + strand, within GFF3859 at 108.436 kb on + strand, within GFF3859 at 108.436 kb on + strand, within GFF3859 at 108.436 kb on + strand, within GFF3859 at 108.437 kb on - strand, within GFF3859 at 108.586 kb on - strand, within GFF3859 at 108.586 kb on - strand, within GFF3859 at 108.747 kb on + strand, within GFF3859 at 108.747 kb on + strand, within GFF3859 at 108.747 kb on + strand, within GFF3859 at 108.747 kb on + strand, within GFF3859 at 108.747 kb on + strand, within GFF3859 at 108.747 kb on + strand, within GFF3859 at 108.747 kb on + strand, within GFF3859 at 108.748 kb on - strand, within GFF3859 at 109.197 kb on - strand, within GFF3859 at 109.273 kb on + strand, within GFF3859 at 109.274 kb on - strand, within GFF3859 at 109.274 kb on - strand, within GFF3859 at 109.274 kb on - strand, within GFF3859 at 109.274 kb on - strand, within GFF3859 at 109.274 kb on - strand, within GFF3859 at 109.330 kb on - strand, within GFF3859 at 109.380 kb on + strand, within GFF3859 at 109.381 kb on - strand, within GFF3859 at 109.381 kb on - strand, within GFF3859 at 109.381 kb on - strand, within GFF3859 at 109.629 kb on + strand at 109.629 kb on + strand at 109.668 kb on + strand at 109.668 kb on + strand at 109.668 kb on + strand at 109.855 kb on + strand at 109.855 kb on + strand at 109.856 kb on - strand at 109.856 kb on - strand at 109.856 kb on - strand at 109.879 kb on + strand at 109.879 kb on + strand at 109.880 kb on - strand at 109.903 kb on - strand at 109.903 kb on - strand at 109.903 kb on - strand at 109.914 kb on + strand at 109.926 kb on + strand at 109.926 kb on + strand at 109.926 kb on + strand at 110.071 kb on + strand, within GFF3860 at 110.152 kb on + strand, within GFF3860 at 110.152 kb on + strand, within GFF3860 at 110.152 kb on + strand, within GFF3860 at 110.152 kb on + strand, within GFF3860 at 110.463 kb on + strand, within GFF3860 at 110.666 kb on + strand, within GFF3860 at 110.696 kb on + strand, within GFF3860 at 110.696 kb on + strand, within GFF3860 at 110.696 kb on + strand, within GFF3860
Per-strain Table
Position Strand Gene LocusTag Fraction Mixed culture; Methylosinus sp. DMC45 1:1 with Pseudomonas_DMC3_ML3; dNMS with methane remove 107,031 - GFF3858 0.69 -2.0 107,031 - GFF3858 0.69 -2.7 107,031 - GFF3858 0.69 -1.1 107,058 + GFF3858 0.71 -3.9 107,059 - GFF3858 0.71 -0.8 107,059 - GFF3858 0.71 -2.9 107,059 - GFF3858 0.71 -1.9 107,389 + -0.0 107,400 + -3.1 107,400 + -1.2 107,400 + -1.5 107,400 + -1.5 107,401 - -2.1 107,401 - +0.2 107,439 + -1.8 107,439 + -1.7 107,439 + -0.4 107,440 - -2.4 107,440 - -0.0 107,440 - -2.8 107,440 - -4.5 107,440 - -2.8 107,440 - -1.3 107,440 - -4.3 107,448 + -2.5 107,449 - -1.9 107,449 - -0.2 107,526 + -3.3 107,526 + -0.8 107,526 + -2.5 107,527 - +0.2 107,527 - -0.8 107,527 - +0.4 107,530 + -1.4 107,530 + -1.5 107,530 + -1.0 107,534 - -1.4 107,560 + -1.7 107,566 + -2.9 107,684 - -1.2 107,800 + -0.8 107,801 - -1.9 107,801 - -1.4 107,801 - -3.4 107,801 - -1.1 107,932 + -1.9 107,933 - -1.0 107,933 - -1.0 108,216 + GFF3859 0.21 -2.0 108,436 + GFF3859 0.33 -1.8 108,436 + GFF3859 0.33 -2.8 108,436 + GFF3859 0.33 -2.0 108,437 - GFF3859 0.33 -1.0 108,586 - GFF3859 0.41 -1.8 108,586 - GFF3859 0.41 -2.7 108,747 + GFF3859 0.49 -1.3 108,747 + GFF3859 0.49 -0.9 108,747 + GFF3859 0.49 -1.5 108,747 + GFF3859 0.49 -2.0 108,747 + GFF3859 0.49 -3.1 108,747 + GFF3859 0.49 -2.4 108,747 + GFF3859 0.49 -0.1 108,748 - GFF3859 0.49 -1.9 109,197 - GFF3859 0.73 -2.1 109,273 + GFF3859 0.77 -0.8 109,274 - GFF3859 0.77 -3.4 109,274 - GFF3859 0.77 -0.4 109,274 - GFF3859 0.77 -1.4 109,274 - GFF3859 0.77 -0.8 109,274 - GFF3859 0.77 -1.5 109,330 - GFF3859 0.80 -3.1 109,380 + GFF3859 0.82 -2.2 109,381 - GFF3859 0.82 -1.4 109,381 - GFF3859 0.82 -1.7 109,381 - GFF3859 0.82 -1.9 109,629 + -1.4 109,629 + -1.9 109,668 + -1.9 109,668 + -2.8 109,668 + -1.6 109,855 + -0.7 109,855 + -0.5 109,856 - +0.0 109,856 - -0.4 109,856 - -0.2 109,879 + +0.5 109,879 + -0.5 109,880 - -0.2 109,903 - -0.6 109,903 - +1.3 109,903 - +1.0 109,914 + +0.2 109,926 + -0.4 109,926 + -1.1 109,926 + -1.4 110,071 + GFF3860 0.12 -0.4 110,152 + GFF3860 0.18 -0.7 110,152 + GFF3860 0.18 -0.2 110,152 + GFF3860 0.18 +0.8 110,152 + GFF3860 0.18 +0.1 110,463 + GFF3860 0.41 +0.3 110,666 + GFF3860 0.56 -0.3 110,696 + GFF3860 0.58 -0.3 110,696 + GFF3860 0.58 -1.0 110,696 + GFF3860 0.58 -3.1
Or see this region's nucleotide sequence