Experiment: Mixed culture; Methylosinus sp. DMC45 1:1 with Pseudomonas_DMC3_ML3; dNMS with methane
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF300 and GFF301 are separated by 139 nucleotides GFF301 and GFF302 are separated by 270 nucleotides
GFF300 - Taurine-binding periplasmic protein, at 334,640 to 335,626
GFF300
GFF301 - Amino-acid acetyltransferase, at 335,766 to 337,067
GFF301
GFF302 - Acetylornithine deacetylase, at 337,338 to 338,483
GFF302
Position (kb) 335
336
337
338 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 334.781 kb on + strand, within GFF300 at 334.782 kb on - strand, within GFF300 at 335.057 kb on + strand, within GFF300 at 335.058 kb on - strand, within GFF300 at 335.095 kb on + strand, within GFF300 at 335.095 kb on + strand, within GFF300 at 335.095 kb on + strand, within GFF300 at 335.096 kb on - strand, within GFF300 at 335.096 kb on - strand, within GFF300 at 335.357 kb on + strand, within GFF300 at 335.588 kb on + strand at 335.588 kb on + strand at 335.653 kb on + strand at 335.654 kb on - strand at 335.682 kb on + strand at 335.682 kb on + strand at 335.682 kb on + strand at 335.682 kb on + strand at 335.682 kb on + strand at 335.795 kb on + strand at 335.795 kb on + strand at 335.796 kb on - strand at 335.796 kb on - strand at 335.803 kb on + strand at 335.803 kb on + strand at 335.803 kb on + strand at 335.803 kb on + strand at 335.803 kb on + strand at 335.803 kb on + strand at 335.803 kb on + strand at 335.804 kb on - strand at 335.804 kb on - strand at 335.804 kb on - strand at 335.804 kb on - strand at 335.804 kb on - strand at 335.804 kb on - strand at 335.809 kb on + strand at 335.809 kb on + strand at 335.810 kb on - strand at 336.037 kb on + strand, within GFF301 at 336.296 kb on + strand, within GFF301 at 336.358 kb on + strand, within GFF301 at 336.358 kb on + strand, within GFF301 at 336.359 kb on - strand, within GFF301 at 336.417 kb on + strand, within GFF301 at 336.418 kb on - strand, within GFF301 at 336.418 kb on - strand, within GFF301 at 336.571 kb on - strand, within GFF301 at 336.643 kb on + strand, within GFF301 at 336.643 kb on + strand, within GFF301 at 336.643 kb on + strand, within GFF301 at 336.838 kb on + strand, within GFF301 at 336.838 kb on + strand, within GFF301 at 336.839 kb on - strand, within GFF301 at 337.027 kb on + strand at 337.027 kb on + strand at 337.027 kb on + strand at 337.028 kb on - strand at 337.028 kb on - strand at 337.028 kb on - strand at 337.028 kb on - strand at 337.028 kb on - strand at 337.052 kb on + strand at 337.052 kb on + strand at 337.052 kb on + strand at 337.053 kb on - strand at 337.057 kb on + strand at 337.057 kb on + strand at 337.057 kb on + strand at 337.058 kb on - strand at 337.081 kb on + strand at 337.082 kb on - strand at 337.084 kb on + strand at 337.084 kb on + strand at 337.084 kb on + strand at 337.084 kb on + strand at 337.084 kb on + strand at 337.085 kb on - strand at 337.085 kb on - strand at 337.085 kb on - strand at 337.085 kb on - strand at 337.182 kb on + strand at 337.182 kb on + strand at 337.182 kb on + strand at 337.182 kb on + strand at 337.205 kb on + strand at 337.218 kb on + strand at 337.271 kb on - strand at 337.324 kb on + strand at 337.324 kb on + strand at 337.324 kb on + strand at 337.325 kb on - strand at 337.354 kb on + strand at 337.354 kb on + strand at 337.389 kb on - strand at 337.415 kb on - strand at 337.489 kb on + strand, within GFF302 at 337.490 kb on - strand, within GFF302 at 337.628 kb on + strand, within GFF302 at 337.628 kb on + strand, within GFF302 at 337.628 kb on + strand, within GFF302 at 337.629 kb on - strand, within GFF302
Per-strain Table
Position Strand Gene LocusTag Fraction Mixed culture; Methylosinus sp. DMC45 1:1 with Pseudomonas_DMC3_ML3; dNMS with methane remove 334,781 + GFF300 0.14 -0.4 334,782 - GFF300 0.14 -1.0 335,057 + GFF300 0.42 -1.5 335,058 - GFF300 0.42 +0.1 335,095 + GFF300 0.46 +0.0 335,095 + GFF300 0.46 +0.7 335,095 + GFF300 0.46 -0.2 335,096 - GFF300 0.46 +0.6 335,096 - GFF300 0.46 +0.7 335,357 + GFF300 0.73 -0.3 335,588 + +0.5 335,588 + +0.0 335,653 + -0.1 335,654 - +0.2 335,682 + +0.6 335,682 + -0.7 335,682 + -1.2 335,682 + -0.5 335,682 + -0.2 335,795 + -3.5 335,795 + -2.0 335,796 - -1.2 335,796 - -1.0 335,803 + -0.4 335,803 + -2.0 335,803 + -0.4 335,803 + -0.7 335,803 + -2.9 335,803 + -2.3 335,803 + -1.1 335,804 - -0.7 335,804 - -0.6 335,804 - -1.9 335,804 - -2.4 335,804 - -1.6 335,804 - -0.9 335,809 + -1.4 335,809 + -1.0 335,810 - -1.6 336,037 + GFF301 0.21 -2.9 336,296 + GFF301 0.41 -1.2 336,358 + GFF301 0.45 -1.3 336,358 + GFF301 0.45 -1.6 336,359 - GFF301 0.46 -2.6 336,417 + GFF301 0.50 -4.1 336,418 - GFF301 0.50 -1.6 336,418 - GFF301 0.50 -2.0 336,571 - GFF301 0.62 -2.4 336,643 + GFF301 0.67 -2.3 336,643 + GFF301 0.67 -0.7 336,643 + GFF301 0.67 -2.2 336,838 + GFF301 0.82 -3.4 336,838 + GFF301 0.82 -1.6 336,839 - GFF301 0.82 -2.2 337,027 + -1.0 337,027 + -0.9 337,027 + -0.6 337,028 - -3.7 337,028 - -3.2 337,028 - -1.9 337,028 - -2.5 337,028 - -1.7 337,052 + -2.7 337,052 + -2.2 337,052 + -2.6 337,053 - -2.7 337,057 + -2.3 337,057 + -1.3 337,057 + -1.6 337,058 - -0.7 337,081 + -1.2 337,082 - +0.4 337,084 + -0.6 337,084 + -2.7 337,084 + -1.6 337,084 + -3.2 337,084 + -0.4 337,085 - +0.2 337,085 - +0.3 337,085 - -0.5 337,085 - +0.6 337,182 + -0.3 337,182 + +0.0 337,182 + +0.7 337,182 + +0.6 337,205 + +0.1 337,218 + +0.4 337,271 - +1.2 337,324 + -0.7 337,324 + +0.2 337,324 + -0.9 337,325 - -0.9 337,354 + +1.2 337,354 + +0.2 337,389 - -0.6 337,415 - -0.8 337,489 + GFF302 0.13 +1.5 337,490 - GFF302 0.13 +0.0 337,628 + GFF302 0.25 -0.5 337,628 + GFF302 0.25 -0.3 337,628 + GFF302 0.25 +1.0 337,629 - GFF302 0.25 -0.6
Or see this region's nucleotide sequence