Experiment: Z3 MOI1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt OHPLBJKB_04421 and OHPLBJKB_04422 are separated by 65 nucleotides OHPLBJKB_04422 and OHPLBJKB_04423 are separated by 56 nucleotides OHPLBJKB_04423 and OHPLBJKB_04424 are separated by 805 nucleotides OHPLBJKB_04424 and OHPLBJKB_04425 overlap by 4 nucleotides
OHPLBJKB_04421: OHPLBJKB_04421 - Nucleoid occlusion protein, at 72,611 to 74,569
_04421
OHPLBJKB_04422: OHPLBJKB_04422 - hypothetical protein, at 74,635 to 74,868
_04422
OHPLBJKB_04423: OHPLBJKB_04423 - Plasmid-derived single-stranded DNA-binding protein, at 74,925 to 75,464
_04423
OHPLBJKB_04424: OHPLBJKB_04424 - hypothetical protein, at 76,270 to 76,461
_04424
OHPLBJKB_04425: OHPLBJKB_04425 - hypothetical protein, at 76,458 to 76,880
_04425
Position (kb) 74
75
76 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 74.437 kb on + strand at 74.437 kb on + strand at 74.437 kb on + strand at 74.551 kb on + strand at 74.551 kb on + strand at 74.552 kb on - strand at 74.569 kb on + strand at 74.569 kb on + strand at 74.570 kb on - strand at 74.570 kb on - strand at 74.570 kb on - strand at 74.570 kb on - strand at 74.573 kb on + strand at 74.573 kb on + strand at 74.573 kb on + strand at 74.573 kb on + strand at 74.573 kb on + strand at 74.573 kb on + strand at 74.573 kb on + strand at 74.573 kb on + strand at 74.574 kb on - strand at 74.574 kb on - strand at 74.574 kb on - strand at 74.574 kb on - strand at 74.576 kb on + strand at 74.576 kb on + strand at 74.576 kb on + strand at 74.577 kb on - strand at 74.594 kb on + strand at 74.595 kb on - strand at 74.637 kb on - strand at 74.640 kb on + strand at 74.640 kb on + strand at 74.641 kb on - strand at 74.641 kb on - strand at 74.641 kb on - strand at 74.641 kb on - strand at 74.641 kb on - strand at 74.707 kb on - strand, within OHPLBJKB_04422 at 74.727 kb on + strand, within OHPLBJKB_04422 at 74.727 kb on + strand, within OHPLBJKB_04422 at 74.848 kb on + strand at 74.848 kb on + strand at 74.849 kb on - strand at 75.002 kb on + strand, within OHPLBJKB_04423 at 75.002 kb on + strand, within OHPLBJKB_04423 at 75.002 kb on + strand, within OHPLBJKB_04423 at 75.003 kb on - strand, within OHPLBJKB_04423 at 75.126 kb on + strand, within OHPLBJKB_04423 at 75.126 kb on + strand, within OHPLBJKB_04423 at 75.127 kb on - strand, within OHPLBJKB_04423 at 75.155 kb on + strand, within OHPLBJKB_04423 at 75.229 kb on + strand, within OHPLBJKB_04423 at 75.230 kb on - strand, within OHPLBJKB_04423 at 75.397 kb on + strand, within OHPLBJKB_04423 at 75.398 kb on - strand, within OHPLBJKB_04423 at 75.398 kb on - strand, within OHPLBJKB_04423 at 75.429 kb on + strand at 75.697 kb on + strand at 76.268 kb on + strand at 76.269 kb on - strand at 76.298 kb on + strand, within OHPLBJKB_04424 at 76.298 kb on + strand, within OHPLBJKB_04424 at 76.298 kb on + strand, within OHPLBJKB_04424 at 76.299 kb on - strand, within OHPLBJKB_04424 at 76.299 kb on - strand, within OHPLBJKB_04424 at 76.299 kb on - strand, within OHPLBJKB_04424 at 76.421 kb on + strand, within OHPLBJKB_04424 at 76.455 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Z3 MOI1 remove 74,437 + +0.2 74,437 + +0.9 74,437 + +0.7 74,551 + +0.3 74,551 + -0.3 74,552 - +0.7 74,569 + -0.3 74,569 + +0.3 74,570 - +0.6 74,570 - -2.3 74,570 - -1.4 74,570 - -0.6 74,573 + +1.0 74,573 + -0.2 74,573 + -0.7 74,573 + +1.1 74,573 + +0.3 74,573 + +1.8 74,573 + -0.7 74,573 + -1.2 74,574 - -0.5 74,574 - +2.9 74,574 - -0.8 74,574 - -0.5 74,576 + +0.2 74,576 + -0.0 74,576 + -0.3 74,577 - -1.2 74,594 + +0.5 74,595 - +0.5 74,637 - -2.3 74,640 + -1.0 74,640 + +0.1 74,641 - -0.7 74,641 - -1.5 74,641 - -2.0 74,641 - +1.1 74,641 - -1.2 74,707 - OHPLBJKB_04422 0.31 -0.0 74,727 + OHPLBJKB_04422 0.39 +0.8 74,727 + OHPLBJKB_04422 0.39 -1.1 74,848 + +0.1 74,848 + +0.7 74,849 - -0.0 75,002 + OHPLBJKB_04423 0.14 -0.8 75,002 + OHPLBJKB_04423 0.14 +1.1 75,002 + OHPLBJKB_04423 0.14 +0.7 75,003 - OHPLBJKB_04423 0.14 +0.3 75,126 + OHPLBJKB_04423 0.37 -0.8 75,126 + OHPLBJKB_04423 0.37 -0.3 75,127 - OHPLBJKB_04423 0.37 +0.7 75,155 + OHPLBJKB_04423 0.43 +0.1 75,229 + OHPLBJKB_04423 0.56 +0.6 75,230 - OHPLBJKB_04423 0.56 -0.7 75,397 + OHPLBJKB_04423 0.87 -0.0 75,398 - OHPLBJKB_04423 0.88 +0.5 75,398 - OHPLBJKB_04423 0.88 +0.7 75,429 + -0.0 75,697 + +0.1 76,268 + -0.0 76,269 - -0.3 76,298 + OHPLBJKB_04424 0.15 +0.2 76,298 + OHPLBJKB_04424 0.15 +2.3 76,298 + OHPLBJKB_04424 0.15 +2.3 76,299 - OHPLBJKB_04424 0.15 -1.4 76,299 - OHPLBJKB_04424 0.15 -0.3 76,299 - OHPLBJKB_04424 0.15 +1.4 76,421 + OHPLBJKB_04424 0.79 -0.5 76,455 + -0.6
Or see this region's nucleotide sequence