Strain Fitness in Escherichia coli HS(pFamp)R (ATCC 700891) around OHPLBJKB_01194

Experiment: BWC1S MOI1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntOHPLBJKB_01193 and OHPLBJKB_01194 are separated by 26 nucleotidesOHPLBJKB_01194 and OHPLBJKB_01195 are separated by 110 nucleotides OHPLBJKB_01193: OHPLBJKB_01193 - Cytoskeleton protein RodZ, at 1,230,143 to 1,231,156 _01193 OHPLBJKB_01194: OHPLBJKB_01194 - 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (flavodoxin), at 1,231,183 to 1,232,301 _01194 OHPLBJKB_01195: OHPLBJKB_01195 - Histidine--tRNA ligase, at 1,232,412 to 1,233,686 _01195 Position (kb) 1231 1232 1233Strain fitness (log2 ratio) -2 -1 0 1at 1230.610 kb on + strand, within OHPLBJKB_01193at 1230.639 kb on + strand, within OHPLBJKB_01193at 1230.643 kb on + strand, within OHPLBJKB_01193at 1230.643 kb on + strand, within OHPLBJKB_01193at 1230.655 kb on + strand, within OHPLBJKB_01193at 1230.655 kb on + strand, within OHPLBJKB_01193at 1230.655 kb on + strand, within OHPLBJKB_01193at 1230.667 kb on + strand, within OHPLBJKB_01193at 1230.799 kb on + strand, within OHPLBJKB_01193at 1230.919 kb on + strand, within OHPLBJKB_01193at 1231.005 kb on + strand, within OHPLBJKB_01193at 1231.053 kb on + strand, within OHPLBJKB_01193at 1231.053 kb on + strand, within OHPLBJKB_01193at 1232.300 kb on - strandat 1232.300 kb on - strandat 1232.300 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction BWC1S MOI1
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1,230,610 + OHPLBJKB_01193 0.46 -0.7
1,230,639 + OHPLBJKB_01193 0.49 -2.0
1,230,643 + OHPLBJKB_01193 0.49 -0.5
1,230,643 + OHPLBJKB_01193 0.49 -1.4
1,230,655 + OHPLBJKB_01193 0.50 -1.5
1,230,655 + OHPLBJKB_01193 0.50 +0.0
1,230,655 + OHPLBJKB_01193 0.50 -0.5
1,230,667 + OHPLBJKB_01193 0.52 +0.2
1,230,799 + OHPLBJKB_01193 0.65 +1.4
1,230,919 + OHPLBJKB_01193 0.77 +0.6
1,231,005 + OHPLBJKB_01193 0.85 +1.0
1,231,053 + OHPLBJKB_01193 0.90 -0.9
1,231,053 + OHPLBJKB_01193 0.90 -2.5
1,232,300 - -1.0
1,232,300 - -0.8
1,232,300 - -0.4

Or see this region's nucleotide sequence