Experiment: R2A_PIPES with Cadmium chloride 128 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pyrG and pepP are separated by 145 nucleotides pepP and cysK are separated by 194 nucleotides
OKGIIK_08165: pyrG - CTP synthase, at 1,793,796 to 1,795,466
pyrG
OKGIIK_08170: pepP - Xaa-Pro aminopeptidase, at 1,795,612 to 1,797,411
pepP
OKGIIK_08175: cysK - pyridoxal-5-phosphate-dependent protein subunit beta, at 1,797,606 to 1,798,556
cysK
Position (kb)
1795
1796
1797
1798 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1795.498 kb on - strand at 1795.515 kb on - strand at 1795.515 kb on - strand at 1795.531 kb on - strand at 1795.600 kb on + strand at 1795.600 kb on + strand at 1795.600 kb on + strand at 1795.600 kb on + strand at 1795.600 kb on + strand at 1795.600 kb on + strand at 1795.600 kb on + strand at 1795.600 kb on + strand at 1795.600 kb on + strand at 1795.600 kb on + strand at 1795.600 kb on + strand at 1795.600 kb on + strand at 1795.600 kb on + strand at 1795.600 kb on + strand at 1795.600 kb on + strand at 1795.600 kb on + strand at 1795.600 kb on + strand at 1795.600 kb on + strand at 1795.600 kb on + strand at 1795.600 kb on + strand at 1795.600 kb on + strand at 1795.600 kb on + strand at 1795.600 kb on + strand at 1795.601 kb on - strand at 1795.601 kb on - strand at 1795.601 kb on - strand at 1795.601 kb on - strand at 1795.601 kb on - strand at 1795.601 kb on - strand at 1795.601 kb on - strand at 1795.601 kb on - strand at 1795.601 kb on - strand at 1795.601 kb on - strand at 1795.601 kb on - strand at 1795.601 kb on - strand at 1795.601 kb on - strand at 1795.601 kb on - strand at 1795.601 kb on - strand at 1795.717 kb on + strand at 1795.718 kb on - strand at 1795.825 kb on + strand, within pepP at 1795.825 kb on + strand, within pepP at 1796.374 kb on + strand, within pepP at 1796.374 kb on + strand, within pepP at 1796.374 kb on + strand, within pepP at 1796.374 kb on + strand, within pepP at 1796.374 kb on + strand, within pepP at 1796.374 kb on + strand, within pepP at 1796.374 kb on + strand, within pepP at 1796.375 kb on - strand, within pepP at 1796.375 kb on - strand, within pepP at 1796.752 kb on + strand, within pepP at 1796.752 kb on + strand, within pepP at 1796.753 kb on - strand, within pepP at 1796.753 kb on - strand, within pepP at 1796.753 kb on - strand, within pepP at 1796.753 kb on - strand, within pepP at 1796.824 kb on + strand, within pepP at 1796.824 kb on + strand, within pepP at 1796.824 kb on + strand, within pepP at 1796.824 kb on + strand, within pepP at 1796.824 kb on + strand, within pepP at 1796.824 kb on + strand, within pepP at 1796.824 kb on + strand, within pepP at 1796.824 kb on + strand, within pepP at 1796.825 kb on - strand, within pepP at 1796.825 kb on - strand, within pepP at 1796.825 kb on - strand, within pepP at 1796.825 kb on - strand, within pepP at 1796.825 kb on - strand, within pepP at 1796.825 kb on - strand, within pepP at 1796.825 kb on - strand, within pepP at 1796.825 kb on - strand, within pepP at 1796.825 kb on - strand, within pepP at 1796.906 kb on + strand, within pepP at 1796.906 kb on + strand, within pepP at 1796.906 kb on + strand, within pepP at 1796.906 kb on + strand, within pepP at 1796.907 kb on - strand, within pepP at 1796.907 kb on - strand, within pepP at 1796.907 kb on - strand, within pepP at 1797.013 kb on + strand, within pepP at 1797.013 kb on + strand, within pepP at 1797.013 kb on + strand, within pepP at 1797.013 kb on + strand, within pepP at 1797.014 kb on - strand, within pepP at 1797.014 kb on - strand, within pepP at 1797.014 kb on - strand, within pepP at 1797.014 kb on - strand, within pepP at 1797.014 kb on - strand, within pepP at 1797.014 kb on - strand, within pepP at 1797.014 kb on - strand, within pepP at 1797.014 kb on - strand, within pepP at 1797.014 kb on - strand, within pepP at 1797.043 kb on + strand, within pepP at 1797.043 kb on + strand, within pepP at 1797.043 kb on + strand, within pepP at 1797.043 kb on + strand, within pepP at 1797.043 kb on + strand, within pepP at 1797.043 kb on + strand, within pepP at 1797.043 kb on + strand, within pepP at 1797.043 kb on + strand, within pepP at 1797.044 kb on - strand, within pepP at 1797.044 kb on - strand, within pepP at 1797.151 kb on + strand, within pepP at 1797.151 kb on + strand, within pepP at 1797.151 kb on + strand, within pepP at 1797.151 kb on + strand, within pepP at 1797.151 kb on + strand, within pepP at 1797.151 kb on + strand, within pepP at 1797.151 kb on + strand, within pepP at 1797.151 kb on + strand, within pepP at 1797.151 kb on + strand, within pepP at 1797.151 kb on + strand, within pepP at 1797.151 kb on + strand, within pepP at 1797.151 kb on + strand, within pepP at 1797.151 kb on + strand, within pepP at 1797.151 kb on + strand, within pepP at 1797.151 kb on + strand, within pepP at 1797.151 kb on + strand, within pepP at 1797.152 kb on - strand, within pepP at 1797.152 kb on - strand, within pepP at 1797.152 kb on - strand, within pepP at 1797.152 kb on - strand, within pepP at 1797.152 kb on - strand, within pepP at 1797.152 kb on - strand, within pepP at 1797.152 kb on - strand, within pepP at 1797.152 kb on - strand, within pepP at 1797.152 kb on - strand, within pepP at 1797.152 kb on - strand, within pepP at 1797.152 kb on - strand, within pepP at 1797.152 kb on - strand, within pepP at 1797.152 kb on - strand, within pepP at 1797.152 kb on - strand, within pepP at 1797.152 kb on - strand, within pepP at 1797.152 kb on - strand, within pepP at 1797.152 kb on - strand, within pepP at 1797.152 kb on - strand, within pepP at 1797.152 kb on - strand, within pepP at 1797.152 kb on - strand, within pepP at 1797.152 kb on - strand, within pepP at 1797.325 kb on + strand at 1797.326 kb on - strand at 1797.326 kb on - strand at 1797.326 kb on - strand at 1797.409 kb on + strand at 1797.409 kb on + strand at 1797.409 kb on + strand at 1797.409 kb on + strand at 1797.409 kb on + strand at 1797.409 kb on + strand at 1797.409 kb on + strand at 1797.409 kb on + strand at 1797.409 kb on + strand at 1797.409 kb on + strand at 1797.410 kb on - strand at 1797.410 kb on - strand at 1797.410 kb on - strand at 1797.410 kb on - strand at 1797.410 kb on - strand at 1797.410 kb on - strand at 1797.410 kb on - strand at 1797.410 kb on - strand at 1797.410 kb on - strand at 1797.420 kb on - strand at 1797.420 kb on - strand at 1797.420 kb on - strand at 1797.488 kb on + strand at 1797.488 kb on + strand at 1797.488 kb on + strand at 1797.488 kb on + strand at 1797.488 kb on + strand at 1797.488 kb on + strand at 1797.488 kb on + strand at 1797.489 kb on - strand at 1797.489 kb on - strand at 1797.489 kb on - strand at 1797.489 kb on - strand at 1797.489 kb on - strand at 1797.489 kb on - strand at 1797.489 kb on - strand at 1797.489 kb on - strand at 1797.489 kb on - strand at 1797.514 kb on + strand at 1797.515 kb on - strand at 1797.607 kb on + strand at 1797.607 kb on + strand at 1797.655 kb on + strand at 1797.655 kb on + strand at 1797.655 kb on + strand at 1797.655 kb on + strand at 1797.655 kb on + strand at 1797.655 kb on + strand at 1797.655 kb on + strand at 1797.655 kb on + strand at 1797.655 kb on + strand at 1797.655 kb on + strand at 1797.655 kb on + strand at 1797.655 kb on + strand at 1797.655 kb on + strand at 1797.655 kb on + strand at 1797.655 kb on + strand at 1797.656 kb on - strand at 1797.656 kb on - strand at 1797.656 kb on - strand at 1797.656 kb on - strand at 1797.656 kb on - strand at 1797.656 kb on - strand at 1797.656 kb on - strand at 1797.656 kb on - strand at 1797.656 kb on - strand at 1797.656 kb on - strand at 1797.656 kb on - strand at 1797.667 kb on + strand at 1797.667 kb on + strand at 1797.667 kb on + strand at 1797.667 kb on + strand at 1797.667 kb on + strand at 1797.667 kb on + strand at 1797.667 kb on + strand at 1797.667 kb on + strand at 1797.667 kb on + strand at 1797.667 kb on + strand at 1797.667 kb on + strand at 1797.667 kb on + strand at 1797.667 kb on + strand at 1797.668 kb on - strand at 1797.668 kb on - strand at 1797.668 kb on - strand at 1797.668 kb on - strand at 1797.668 kb on - strand at 1797.668 kb on - strand at 1797.668 kb on - strand at 1797.668 kb on - strand at 1797.911 kb on + strand, within cysK at 1797.911 kb on + strand, within cysK at 1797.911 kb on + strand, within cysK at 1797.911 kb on + strand, within cysK at 1797.911 kb on + strand, within cysK at 1797.911 kb on + strand, within cysK at 1797.912 kb on - strand, within cysK at 1797.912 kb on - strand, within cysK at 1798.035 kb on + strand, within cysK at 1798.035 kb on + strand, within cysK at 1798.035 kb on + strand, within cysK at 1798.156 kb on + strand, within cysK at 1798.156 kb on + strand, within cysK at 1798.156 kb on + strand, within cysK at 1798.156 kb on + strand, within cysK at 1798.157 kb on - strand, within cysK at 1798.157 kb on - strand, within cysK at 1798.157 kb on - strand, within cysK at 1798.157 kb on - strand, within cysK at 1798.157 kb on - strand, within cysK at 1798.157 kb on - strand, within cysK at 1798.240 kb on + strand, within cysK at 1798.240 kb on + strand, within cysK at 1798.240 kb on + strand, within cysK at 1798.343 kb on + strand, within cysK at 1798.343 kb on + strand, within cysK at 1798.344 kb on - strand, within cysK at 1798.344 kb on - strand, within cysK
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Cadmium chloride 128 uM remove 1,795,498 - -0.5 1,795,515 - -1.3 1,795,515 - +0.3 1,795,531 - -0.1 1,795,600 + -1.3 1,795,600 + -0.6 1,795,600 + -0.1 1,795,600 + +0.7 1,795,600 + -1.5 1,795,600 + -1.1 1,795,600 + -1.1 1,795,600 + -2.1 1,795,600 + -1.9 1,795,600 + -1.2 1,795,600 + -0.2 1,795,600 + -0.6 1,795,600 + +1.2 1,795,600 + -0.6 1,795,600 + -0.9 1,795,600 + -2.9 1,795,600 + -0.3 1,795,600 + -1.5 1,795,600 + -0.9 1,795,600 + -1.3 1,795,600 + -1.0 1,795,600 + -0.1 1,795,600 + -0.9 1,795,601 - -0.8 1,795,601 - -2.3 1,795,601 - -1.0 1,795,601 - -2.2 1,795,601 - -0.3 1,795,601 - -1.3 1,795,601 - -0.6 1,795,601 - -2.0 1,795,601 - -2.0 1,795,601 - -0.3 1,795,601 - -2.1 1,795,601 - -0.3 1,795,601 - -0.8 1,795,601 - -1.1 1,795,601 - -2.0 1,795,717 + -1.3 1,795,718 - -1.9 1,795,825 + pepP OKGIIK_08170 0.12 -1.3 1,795,825 + pepP OKGIIK_08170 0.12 -1.9 1,796,374 + pepP OKGIIK_08170 0.42 -1.1 1,796,374 + pepP OKGIIK_08170 0.42 -0.3 1,796,374 + pepP OKGIIK_08170 0.42 -1.5 1,796,374 + pepP OKGIIK_08170 0.42 -0.3 1,796,374 + pepP OKGIIK_08170 0.42 -0.3 1,796,374 + pepP OKGIIK_08170 0.42 +0.4 1,796,374 + pepP OKGIIK_08170 0.42 -1.1 1,796,375 - pepP OKGIIK_08170 0.42 -0.9 1,796,375 - pepP OKGIIK_08170 0.42 -1.1 1,796,752 + pepP OKGIIK_08170 0.63 -0.6 1,796,752 + pepP OKGIIK_08170 0.63 -0.3 1,796,753 - pepP OKGIIK_08170 0.63 -1.4 1,796,753 - pepP OKGIIK_08170 0.63 -1.3 1,796,753 - pepP OKGIIK_08170 0.63 -1.3 1,796,753 - pepP OKGIIK_08170 0.63 -1.3 1,796,824 + pepP OKGIIK_08170 0.67 -0.8 1,796,824 + pepP OKGIIK_08170 0.67 -0.6 1,796,824 + pepP OKGIIK_08170 0.67 -1.3 1,796,824 + pepP OKGIIK_08170 0.67 -1.3 1,796,824 + pepP OKGIIK_08170 0.67 +0.7 1,796,824 + pepP OKGIIK_08170 0.67 -1.3 1,796,824 + pepP OKGIIK_08170 0.67 -0.6 1,796,824 + pepP OKGIIK_08170 0.67 +1.5 1,796,825 - pepP OKGIIK_08170 0.67 +0.7 1,796,825 - pepP OKGIIK_08170 0.67 -0.9 1,796,825 - pepP OKGIIK_08170 0.67 -0.6 1,796,825 - pepP OKGIIK_08170 0.67 -0.3 1,796,825 - pepP OKGIIK_08170 0.67 -0.3 1,796,825 - pepP OKGIIK_08170 0.67 +1.3 1,796,825 - pepP OKGIIK_08170 0.67 -1.8 1,796,825 - pepP OKGIIK_08170 0.67 -0.9 1,796,825 - pepP OKGIIK_08170 0.67 -0.9 1,796,906 + pepP OKGIIK_08170 0.72 +0.7 1,796,906 + pepP OKGIIK_08170 0.72 +1.5 1,796,906 + pepP OKGIIK_08170 0.72 -0.3 1,796,906 + pepP OKGIIK_08170 0.72 +0.4 1,796,907 - pepP OKGIIK_08170 0.72 +0.5 1,796,907 - pepP OKGIIK_08170 0.72 -0.1 1,796,907 - pepP OKGIIK_08170 0.72 -0.3 1,797,013 + pepP OKGIIK_08170 0.78 -2.5 1,797,013 + pepP OKGIIK_08170 0.78 +0.5 1,797,013 + pepP OKGIIK_08170 0.78 -1.9 1,797,013 + pepP OKGIIK_08170 0.78 -1.3 1,797,014 - pepP OKGIIK_08170 0.78 -0.9 1,797,014 - pepP OKGIIK_08170 0.78 +0.1 1,797,014 - pepP OKGIIK_08170 0.78 +1.1 1,797,014 - pepP OKGIIK_08170 0.78 +2.0 1,797,014 - pepP OKGIIK_08170 0.78 -0.3 1,797,014 - pepP OKGIIK_08170 0.78 -0.6 1,797,014 - pepP OKGIIK_08170 0.78 +0.1 1,797,014 - pepP OKGIIK_08170 0.78 -0.6 1,797,014 - pepP OKGIIK_08170 0.78 -1.5 1,797,043 + pepP OKGIIK_08170 0.80 -0.6 1,797,043 + pepP OKGIIK_08170 0.80 -1.1 1,797,043 + pepP OKGIIK_08170 0.80 -1.8 1,797,043 + pepP OKGIIK_08170 0.80 -2.0 1,797,043 + pepP OKGIIK_08170 0.80 -0.1 1,797,043 + pepP OKGIIK_08170 0.80 -3.0 1,797,043 + pepP OKGIIK_08170 0.80 -0.6 1,797,043 + pepP OKGIIK_08170 0.80 -1.2 1,797,044 - pepP OKGIIK_08170 0.80 -2.2 1,797,044 - pepP OKGIIK_08170 0.80 -0.9 1,797,151 + pepP OKGIIK_08170 0.85 -2.0 1,797,151 + pepP OKGIIK_08170 0.85 -0.9 1,797,151 + pepP OKGIIK_08170 0.85 -1.6 1,797,151 + pepP OKGIIK_08170 0.85 +0.1 1,797,151 + pepP OKGIIK_08170 0.85 -1.9 1,797,151 + pepP OKGIIK_08170 0.85 -0.3 1,797,151 + pepP OKGIIK_08170 0.85 -1.3 1,797,151 + pepP OKGIIK_08170 0.85 -0.6 1,797,151 + pepP OKGIIK_08170 0.85 -0.4 1,797,151 + pepP OKGIIK_08170 0.85 -0.9 1,797,151 + pepP OKGIIK_08170 0.85 -1.9 1,797,151 + pepP OKGIIK_08170 0.85 -2.2 1,797,151 + pepP OKGIIK_08170 0.85 -2.0 1,797,151 + pepP OKGIIK_08170 0.85 -2.5 1,797,151 + pepP OKGIIK_08170 0.85 -3.5 1,797,151 + pepP OKGIIK_08170 0.85 -0.9 1,797,152 - pepP OKGIIK_08170 0.86 -0.3 1,797,152 - pepP OKGIIK_08170 0.86 -3.1 1,797,152 - pepP OKGIIK_08170 0.86 -1.1 1,797,152 - pepP OKGIIK_08170 0.86 -1.6 1,797,152 - pepP OKGIIK_08170 0.86 +0.5 1,797,152 - pepP OKGIIK_08170 0.86 -2.8 1,797,152 - pepP OKGIIK_08170 0.86 +0.1 1,797,152 - pepP OKGIIK_08170 0.86 -2.1 1,797,152 - pepP OKGIIK_08170 0.86 -1.0 1,797,152 - pepP OKGIIK_08170 0.86 -0.4 1,797,152 - pepP OKGIIK_08170 0.86 -1.9 1,797,152 - pepP OKGIIK_08170 0.86 -2.0 1,797,152 - pepP OKGIIK_08170 0.86 -0.5 1,797,152 - pepP OKGIIK_08170 0.86 -0.3 1,797,152 - pepP OKGIIK_08170 0.86 -0.3 1,797,152 - pepP OKGIIK_08170 0.86 -0.9 1,797,152 - pepP OKGIIK_08170 0.86 +0.7 1,797,152 - pepP OKGIIK_08170 0.86 -2.0 1,797,152 - pepP OKGIIK_08170 0.86 -0.5 1,797,152 - pepP OKGIIK_08170 0.86 -0.6 1,797,152 - pepP OKGIIK_08170 0.86 -1.0 1,797,325 + -1.8 1,797,326 - -0.3 1,797,326 - -2.3 1,797,326 - -1.6 1,797,409 + -1.3 1,797,409 + +0.9 1,797,409 + -0.3 1,797,409 + -1.9 1,797,409 + -0.6 1,797,409 + -0.4 1,797,409 + -0.6 1,797,409 + -0.9 1,797,409 + -0.4 1,797,409 + -1.9 1,797,410 - +1.3 1,797,410 - -0.3 1,797,410 - -1.3 1,797,410 - +0.1 1,797,410 - +2.5 1,797,410 - -1.5 1,797,410 - -0.9 1,797,410 - -1.8 1,797,410 - +0.5 1,797,420 - -0.4 1,797,420 - -0.6 1,797,420 - +2.3 1,797,488 + -2.0 1,797,488 + -1.5 1,797,488 + -1.1 1,797,488 + -0.9 1,797,488 + -1.6 1,797,488 + -0.9 1,797,488 + -1.3 1,797,489 - -0.4 1,797,489 - -0.3 1,797,489 - -0.3 1,797,489 - +1.1 1,797,489 - -0.9 1,797,489 - -1.5 1,797,489 - -2.6 1,797,489 - -0.6 1,797,489 - -0.6 1,797,514 + -0.6 1,797,515 - -1.4 1,797,607 + -3.0 1,797,607 + -1.3 1,797,655 + -1.6 1,797,655 + -0.5 1,797,655 + -1.2 1,797,655 + -0.3 1,797,655 + -1.3 1,797,655 + -1.6 1,797,655 + -0.9 1,797,655 + -1.3 1,797,655 + -0.3 1,797,655 + -1.9 1,797,655 + -2.7 1,797,655 + -1.5 1,797,655 + -1.6 1,797,655 + -0.2 1,797,655 + -1.6 1,797,656 - -0.3 1,797,656 - -0.6 1,797,656 - -0.3 1,797,656 - -1.3 1,797,656 - +0.7 1,797,656 - -1.1 1,797,656 - -0.6 1,797,656 - -2.0 1,797,656 - +2.0 1,797,656 - -1.5 1,797,656 - +1.1 1,797,667 + +1.4 1,797,667 + -0.2 1,797,667 + -1.9 1,797,667 + -0.9 1,797,667 + -0.6 1,797,667 + +1.3 1,797,667 + -1.0 1,797,667 + -1.3 1,797,667 + +0.9 1,797,667 + -2.5 1,797,667 + +0.1 1,797,667 + -0.9 1,797,667 + -0.9 1,797,668 - -2.7 1,797,668 - -1.5 1,797,668 - -0.9 1,797,668 - -0.1 1,797,668 - -1.4 1,797,668 - -0.3 1,797,668 - -0.3 1,797,668 - -0.6 1,797,911 + cysK OKGIIK_08175 0.32 +0.4 1,797,911 + cysK OKGIIK_08175 0.32 -1.5 1,797,911 + cysK OKGIIK_08175 0.32 -2.1 1,797,911 + cysK OKGIIK_08175 0.32 -1.1 1,797,911 + cysK OKGIIK_08175 0.32 -0.2 1,797,911 + cysK OKGIIK_08175 0.32 -0.6 1,797,912 - cysK OKGIIK_08175 0.32 +0.1 1,797,912 - cysK OKGIIK_08175 0.32 -1.1 1,798,035 + cysK OKGIIK_08175 0.45 +1.0 1,798,035 + cysK OKGIIK_08175 0.45 -1.3 1,798,035 + cysK OKGIIK_08175 0.45 +0.4 1,798,156 + cysK OKGIIK_08175 0.58 +1.5 1,798,156 + cysK OKGIIK_08175 0.58 -0.9 1,798,156 + cysK OKGIIK_08175 0.58 -1.3 1,798,156 + cysK OKGIIK_08175 0.58 -0.1 1,798,157 - cysK OKGIIK_08175 0.58 -1.1 1,798,157 - cysK OKGIIK_08175 0.58 +1.3 1,798,157 - cysK OKGIIK_08175 0.58 -1.1 1,798,157 - cysK OKGIIK_08175 0.58 +1.7 1,798,157 - cysK OKGIIK_08175 0.58 +0.7 1,798,157 - cysK OKGIIK_08175 0.58 -0.3 1,798,240 + cysK OKGIIK_08175 0.67 -0.3 1,798,240 + cysK OKGIIK_08175 0.67 +1.3 1,798,240 + cysK OKGIIK_08175 0.67 -0.7 1,798,343 + cysK OKGIIK_08175 0.77 +0.4 1,798,343 + cysK OKGIIK_08175 0.77 -2.0 1,798,344 - cysK OKGIIK_08175 0.78 -0.3 1,798,344 - cysK OKGIIK_08175 0.78 -0.1
Or see this region's nucleotide sequence