Experiment: R2A_PIPES with Cadmium chloride 128 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ptsP and OKGIIK_02240 are separated by 142 nucleotides OKGIIK_02240 and manX overlap by 8 nucleotides manX and rapZ are separated by 43 nucleotides rapZ and hprK are separated by 4 nucleotides
OKGIIK_02235: ptsP - phosphoenolpyruvate--protein phosphotransferase, at 525,065 to 526,807
ptsP
OKGIIK_02240: OKGIIK_02240 - Phosphocarrier protein HPr, at 526,950 to 527,219
_02240
OKGIIK_02245: manX - PTS fructose IIA subunit family protein, at 527,212 to 527,604
manX
OKGIIK_02250: rapZ - RNase adapter RapZ, at 527,648 to 528,550
rapZ
OKGIIK_02255: hprK - HPr(Ser) kinase/phosphatase, at 528,555 to 529,457
hprK
Position (kb) 527
528 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 526.233 kb on + strand, within ptsP at 526.233 kb on + strand, within ptsP at 526.233 kb on + strand, within ptsP at 526.233 kb on + strand, within ptsP at 526.234 kb on - strand, within ptsP at 526.234 kb on - strand, within ptsP at 526.234 kb on - strand, within ptsP at 526.434 kb on + strand, within ptsP at 526.434 kb on + strand, within ptsP at 526.435 kb on - strand, within ptsP at 526.435 kb on - strand, within ptsP at 526.435 kb on - strand, within ptsP at 526.435 kb on - strand, within ptsP at 526.435 kb on - strand, within ptsP at 526.435 kb on - strand, within ptsP at 526.506 kb on + strand, within ptsP at 526.728 kb on + strand at 526.728 kb on + strand at 526.728 kb on + strand at 526.728 kb on + strand at 526.728 kb on + strand at 526.728 kb on + strand at 526.728 kb on + strand at 526.729 kb on - strand at 526.729 kb on - strand at 526.729 kb on - strand at 526.729 kb on - strand at 526.951 kb on + strand at 526.951 kb on + strand at 526.951 kb on + strand at 526.951 kb on + strand at 526.951 kb on + strand at 526.952 kb on - strand at 526.952 kb on - strand at 526.952 kb on - strand at 526.952 kb on - strand at 527.121 kb on + strand, within OKGIIK_02240 at 527.121 kb on + strand, within OKGIIK_02240 at 527.121 kb on + strand, within OKGIIK_02240 at 527.122 kb on - strand, within OKGIIK_02240 at 527.122 kb on - strand, within OKGIIK_02240 at 527.122 kb on - strand, within OKGIIK_02240 at 527.122 kb on - strand, within OKGIIK_02240 at 527.122 kb on - strand, within OKGIIK_02240 at 527.122 kb on - strand, within OKGIIK_02240 at 527.122 kb on - strand, within OKGIIK_02240 at 527.122 kb on - strand, within OKGIIK_02240 at 527.122 kb on - strand, within OKGIIK_02240 at 527.122 kb on - strand, within OKGIIK_02240 at 527.122 kb on - strand, within OKGIIK_02240 at 527.122 kb on - strand, within OKGIIK_02240 at 527.122 kb on - strand, within OKGIIK_02240 at 527.291 kb on + strand, within manX at 527.291 kb on + strand, within manX at 527.291 kb on + strand, within manX at 527.291 kb on + strand, within manX at 527.291 kb on + strand, within manX at 527.291 kb on + strand, within manX at 527.291 kb on + strand, within manX at 527.291 kb on + strand, within manX at 527.291 kb on + strand, within manX at 527.291 kb on + strand, within manX at 527.291 kb on + strand, within manX at 527.291 kb on + strand, within manX at 527.291 kb on + strand, within manX at 527.291 kb on + strand, within manX at 527.291 kb on + strand, within manX at 527.291 kb on + strand, within manX at 527.291 kb on + strand, within manX at 527.291 kb on + strand, within manX at 527.292 kb on - strand, within manX at 527.292 kb on - strand, within manX at 527.292 kb on - strand, within manX at 527.292 kb on - strand, within manX at 527.292 kb on - strand, within manX at 527.292 kb on - strand, within manX at 527.292 kb on - strand, within manX at 527.292 kb on - strand, within manX at 527.292 kb on - strand, within manX at 527.292 kb on - strand, within manX at 527.292 kb on - strand, within manX at 527.292 kb on - strand, within manX at 527.292 kb on - strand, within manX at 527.292 kb on - strand, within manX at 527.292 kb on - strand, within manX at 527.292 kb on - strand, within manX at 527.292 kb on - strand, within manX at 527.292 kb on - strand, within manX at 527.292 kb on - strand, within manX at 527.299 kb on + strand, within manX at 527.299 kb on + strand, within manX at 527.300 kb on - strand, within manX at 527.300 kb on - strand, within manX at 527.300 kb on - strand, within manX at 527.347 kb on + strand, within manX at 527.347 kb on + strand, within manX at 527.347 kb on + strand, within manX at 527.347 kb on + strand, within manX at 527.347 kb on + strand, within manX at 527.347 kb on + strand, within manX at 527.347 kb on + strand, within manX at 527.347 kb on + strand, within manX at 527.347 kb on + strand, within manX at 527.347 kb on + strand, within manX at 527.347 kb on + strand, within manX at 527.347 kb on + strand, within manX at 527.347 kb on + strand, within manX at 527.348 kb on - strand, within manX at 527.348 kb on - strand, within manX at 527.348 kb on - strand, within manX at 527.348 kb on - strand, within manX at 527.348 kb on - strand, within manX at 527.348 kb on - strand, within manX at 527.348 kb on - strand, within manX at 527.348 kb on - strand, within manX at 527.348 kb on - strand, within manX at 527.348 kb on - strand, within manX at 527.348 kb on - strand, within manX at 527.396 kb on + strand, within manX at 527.396 kb on + strand, within manX at 527.396 kb on + strand, within manX at 527.397 kb on - strand, within manX at 527.397 kb on - strand, within manX at 527.724 kb on + strand at 527.724 kb on + strand at 527.724 kb on + strand at 527.724 kb on + strand at 527.724 kb on + strand at 527.725 kb on - strand at 527.725 kb on - strand at 527.725 kb on - strand at 527.772 kb on + strand, within rapZ at 527.772 kb on + strand, within rapZ at 527.772 kb on + strand, within rapZ at 527.772 kb on + strand, within rapZ at 527.772 kb on + strand, within rapZ at 527.772 kb on + strand, within rapZ at 527.772 kb on + strand, within rapZ at 527.772 kb on + strand, within rapZ at 527.773 kb on - strand, within rapZ at 527.773 kb on - strand, within rapZ at 527.773 kb on - strand, within rapZ at 527.773 kb on - strand, within rapZ at 527.773 kb on - strand, within rapZ at 527.773 kb on - strand, within rapZ at 527.773 kb on - strand, within rapZ at 527.773 kb on - strand, within rapZ at 527.773 kb on - strand, within rapZ at 527.773 kb on - strand, within rapZ at 527.773 kb on - strand, within rapZ at 527.773 kb on - strand, within rapZ at 527.773 kb on - strand, within rapZ at 527.773 kb on - strand, within rapZ at 527.773 kb on - strand, within rapZ at 527.838 kb on + strand, within rapZ at 527.838 kb on + strand, within rapZ at 527.838 kb on + strand, within rapZ at 527.838 kb on + strand, within rapZ at 527.838 kb on + strand, within rapZ at 527.838 kb on + strand, within rapZ at 527.838 kb on + strand, within rapZ at 527.838 kb on + strand, within rapZ at 527.838 kb on + strand, within rapZ at 527.838 kb on + strand, within rapZ at 527.838 kb on + strand, within rapZ at 527.838 kb on + strand, within rapZ at 527.838 kb on + strand, within rapZ at 527.838 kb on + strand, within rapZ at 527.838 kb on + strand, within rapZ at 527.838 kb on + strand, within rapZ at 527.838 kb on + strand, within rapZ at 527.838 kb on + strand, within rapZ at 527.838 kb on + strand, within rapZ at 527.839 kb on - strand, within rapZ at 527.839 kb on - strand, within rapZ at 527.839 kb on - strand, within rapZ at 527.839 kb on - strand, within rapZ at 527.839 kb on - strand, within rapZ at 527.839 kb on - strand, within rapZ at 527.839 kb on - strand, within rapZ at 527.839 kb on - strand, within rapZ at 527.839 kb on - strand, within rapZ at 527.839 kb on - strand, within rapZ at 527.839 kb on - strand, within rapZ at 527.839 kb on - strand, within rapZ at 527.839 kb on - strand, within rapZ at 527.839 kb on - strand, within rapZ at 527.839 kb on - strand, within rapZ at 527.839 kb on - strand, within rapZ at 527.839 kb on - strand, within rapZ at 527.839 kb on - strand, within rapZ at 527.839 kb on - strand, within rapZ at 527.839 kb on - strand, within rapZ at 528.033 kb on + strand, within rapZ at 528.169 kb on + strand, within rapZ at 528.170 kb on - strand, within rapZ at 528.423 kb on + strand, within rapZ at 528.423 kb on + strand, within rapZ at 528.423 kb on + strand, within rapZ at 528.423 kb on + strand, within rapZ at 528.423 kb on + strand, within rapZ at 528.423 kb on + strand, within rapZ at 528.423 kb on + strand, within rapZ at 528.423 kb on + strand, within rapZ at 528.424 kb on - strand, within rapZ at 528.424 kb on - strand, within rapZ at 528.424 kb on - strand, within rapZ at 528.424 kb on - strand, within rapZ at 528.424 kb on - strand, within rapZ at 528.424 kb on - strand, within rapZ at 528.424 kb on - strand, within rapZ at 528.424 kb on - strand, within rapZ at 528.424 kb on - strand, within rapZ at 528.424 kb on - strand, within rapZ at 528.424 kb on - strand, within rapZ at 528.424 kb on - strand, within rapZ at 528.424 kb on - strand, within rapZ at 528.424 kb on - strand, within rapZ at 528.424 kb on - strand, within rapZ at 528.424 kb on - strand, within rapZ at 528.424 kb on - strand, within rapZ at 528.424 kb on - strand, within rapZ at 528.458 kb on + strand, within rapZ
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Cadmium chloride 128 uM remove 526,233 + ptsP OKGIIK_02235 0.67 -1.3 526,233 + ptsP OKGIIK_02235 0.67 -0.5 526,233 + ptsP OKGIIK_02235 0.67 -0.4 526,233 + ptsP OKGIIK_02235 0.67 -2.5 526,234 - ptsP OKGIIK_02235 0.67 -0.8 526,234 - ptsP OKGIIK_02235 0.67 -0.6 526,234 - ptsP OKGIIK_02235 0.67 -0.5 526,434 + ptsP OKGIIK_02235 0.79 -1.1 526,434 + ptsP OKGIIK_02235 0.79 -2.3 526,435 - ptsP OKGIIK_02235 0.79 -0.5 526,435 - ptsP OKGIIK_02235 0.79 -1.3 526,435 - ptsP OKGIIK_02235 0.79 -1.9 526,435 - ptsP OKGIIK_02235 0.79 +0.5 526,435 - ptsP OKGIIK_02235 0.79 -1.8 526,435 - ptsP OKGIIK_02235 0.79 -0.0 526,506 + ptsP OKGIIK_02235 0.83 -2.5 526,728 + -1.6 526,728 + -1.8 526,728 + -1.8 526,728 + -0.5 526,728 + -1.0 526,728 + -0.8 526,728 + -0.2 526,729 - -1.3 526,729 - -2.4 526,729 - -1.2 526,729 - +0.2 526,951 + -1.3 526,951 + -0.5 526,951 + -1.8 526,951 + +1.0 526,951 + -0.3 526,952 - -1.2 526,952 - -1.4 526,952 - -1.2 526,952 - -1.0 527,121 + OKGIIK_02240 0.63 -1.6 527,121 + OKGIIK_02240 0.63 -1.8 527,121 + OKGIIK_02240 0.63 -0.6 527,122 - OKGIIK_02240 0.64 -1.0 527,122 - OKGIIK_02240 0.64 -2.3 527,122 - OKGIIK_02240 0.64 +1.3 527,122 - OKGIIK_02240 0.64 -1.5 527,122 - OKGIIK_02240 0.64 -1.5 527,122 - OKGIIK_02240 0.64 -0.5 527,122 - OKGIIK_02240 0.64 +0.5 527,122 - OKGIIK_02240 0.64 -1.7 527,122 - OKGIIK_02240 0.64 -1.3 527,122 - OKGIIK_02240 0.64 -0.8 527,122 - OKGIIK_02240 0.64 -0.9 527,122 - OKGIIK_02240 0.64 -0.8 527,122 - OKGIIK_02240 0.64 -1.3 527,291 + manX OKGIIK_02245 0.20 -2.3 527,291 + manX OKGIIK_02245 0.20 -0.5 527,291 + manX OKGIIK_02245 0.20 -2.0 527,291 + manX OKGIIK_02245 0.20 -0.0 527,291 + manX OKGIIK_02245 0.20 -0.5 527,291 + manX OKGIIK_02245 0.20 -4.0 527,291 + manX OKGIIK_02245 0.20 +0.2 527,291 + manX OKGIIK_02245 0.20 -3.2 527,291 + manX OKGIIK_02245 0.20 -1.9 527,291 + manX OKGIIK_02245 0.20 -1.5 527,291 + manX OKGIIK_02245 0.20 -1.9 527,291 + manX OKGIIK_02245 0.20 -1.5 527,291 + manX OKGIIK_02245 0.20 -0.5 527,291 + manX OKGIIK_02245 0.20 -0.8 527,291 + manX OKGIIK_02245 0.20 -1.3 527,291 + manX OKGIIK_02245 0.20 -1.6 527,291 + manX OKGIIK_02245 0.20 -0.5 527,291 + manX OKGIIK_02245 0.20 -0.5 527,292 - manX OKGIIK_02245 0.20 -2.4 527,292 - manX OKGIIK_02245 0.20 -0.5 527,292 - manX OKGIIK_02245 0.20 -1.0 527,292 - manX OKGIIK_02245 0.20 -1.8 527,292 - manX OKGIIK_02245 0.20 -1.0 527,292 - manX OKGIIK_02245 0.20 +0.5 527,292 - manX OKGIIK_02245 0.20 -1.5 527,292 - manX OKGIIK_02245 0.20 +1.0 527,292 - manX OKGIIK_02245 0.20 -2.4 527,292 - manX OKGIIK_02245 0.20 +1.1 527,292 - manX OKGIIK_02245 0.20 -2.5 527,292 - manX OKGIIK_02245 0.20 -1.5 527,292 - manX OKGIIK_02245 0.20 -1.8 527,292 - manX OKGIIK_02245 0.20 -2.8 527,292 - manX OKGIIK_02245 0.20 -0.3 527,292 - manX OKGIIK_02245 0.20 -1.5 527,292 - manX OKGIIK_02245 0.20 -1.8 527,292 - manX OKGIIK_02245 0.20 -1.6 527,292 - manX OKGIIK_02245 0.20 -0.5 527,299 + manX OKGIIK_02245 0.22 -2.3 527,299 + manX OKGIIK_02245 0.22 -1.0 527,300 - manX OKGIIK_02245 0.22 +1.1 527,300 - manX OKGIIK_02245 0.22 -1.5 527,300 - manX OKGIIK_02245 0.22 -0.8 527,347 + manX OKGIIK_02245 0.34 -0.8 527,347 + manX OKGIIK_02245 0.34 -1.0 527,347 + manX OKGIIK_02245 0.34 -2.8 527,347 + manX OKGIIK_02245 0.34 -3.0 527,347 + manX OKGIIK_02245 0.34 -1.3 527,347 + manX OKGIIK_02245 0.34 -1.5 527,347 + manX OKGIIK_02245 0.34 -1.0 527,347 + manX OKGIIK_02245 0.34 -3.3 527,347 + manX OKGIIK_02245 0.34 -1.5 527,347 + manX OKGIIK_02245 0.34 +0.4 527,347 + manX OKGIIK_02245 0.34 -0.8 527,347 + manX OKGIIK_02245 0.34 -2.0 527,347 + manX OKGIIK_02245 0.34 -1.3 527,348 - manX OKGIIK_02245 0.35 -1.1 527,348 - manX OKGIIK_02245 0.35 -3.5 527,348 - manX OKGIIK_02245 0.35 -1.2 527,348 - manX OKGIIK_02245 0.35 -0.8 527,348 - manX OKGIIK_02245 0.35 -1.5 527,348 - manX OKGIIK_02245 0.35 -0.5 527,348 - manX OKGIIK_02245 0.35 -2.2 527,348 - manX OKGIIK_02245 0.35 +0.5 527,348 - manX OKGIIK_02245 0.35 -1.9 527,348 - manX OKGIIK_02245 0.35 -1.8 527,348 - manX OKGIIK_02245 0.35 -1.0 527,396 + manX OKGIIK_02245 0.47 -1.8 527,396 + manX OKGIIK_02245 0.47 -1.9 527,396 + manX OKGIIK_02245 0.47 -1.3 527,397 - manX OKGIIK_02245 0.47 -1.6 527,397 - manX OKGIIK_02245 0.47 -0.5 527,724 + -0.0 527,724 + -1.0 527,724 + -0.6 527,724 + +0.7 527,724 + -1.3 527,725 - -1.3 527,725 - -0.8 527,725 - -0.5 527,772 + rapZ OKGIIK_02250 0.14 -2.4 527,772 + rapZ OKGIIK_02250 0.14 -1.0 527,772 + rapZ OKGIIK_02250 0.14 -0.8 527,772 + rapZ OKGIIK_02250 0.14 -1.3 527,772 + rapZ OKGIIK_02250 0.14 -3.2 527,772 + rapZ OKGIIK_02250 0.14 -1.0 527,772 + rapZ OKGIIK_02250 0.14 -1.8 527,772 + rapZ OKGIIK_02250 0.14 -0.6 527,773 - rapZ OKGIIK_02250 0.14 -0.5 527,773 - rapZ OKGIIK_02250 0.14 -1.0 527,773 - rapZ OKGIIK_02250 0.14 -1.0 527,773 - rapZ OKGIIK_02250 0.14 -0.5 527,773 - rapZ OKGIIK_02250 0.14 -1.0 527,773 - rapZ OKGIIK_02250 0.14 -2.4 527,773 - rapZ OKGIIK_02250 0.14 -1.5 527,773 - rapZ OKGIIK_02250 0.14 -3.2 527,773 - rapZ OKGIIK_02250 0.14 -0.7 527,773 - rapZ OKGIIK_02250 0.14 +0.3 527,773 - rapZ OKGIIK_02250 0.14 -0.8 527,773 - rapZ OKGIIK_02250 0.14 -2.8 527,773 - rapZ OKGIIK_02250 0.14 -0.5 527,773 - rapZ OKGIIK_02250 0.14 -0.8 527,773 - rapZ OKGIIK_02250 0.14 -0.5 527,838 + rapZ OKGIIK_02250 0.21 -0.8 527,838 + rapZ OKGIIK_02250 0.21 -1.2 527,838 + rapZ OKGIIK_02250 0.21 -1.3 527,838 + rapZ OKGIIK_02250 0.21 -0.8 527,838 + rapZ OKGIIK_02250 0.21 -0.5 527,838 + rapZ OKGIIK_02250 0.21 -0.5 527,838 + rapZ OKGIIK_02250 0.21 -1.6 527,838 + rapZ OKGIIK_02250 0.21 -0.8 527,838 + rapZ OKGIIK_02250 0.21 -1.8 527,838 + rapZ OKGIIK_02250 0.21 -1.0 527,838 + rapZ OKGIIK_02250 0.21 -1.3 527,838 + rapZ OKGIIK_02250 0.21 -1.3 527,838 + rapZ OKGIIK_02250 0.21 -2.5 527,838 + rapZ OKGIIK_02250 0.21 -1.0 527,838 + rapZ OKGIIK_02250 0.21 -1.5 527,838 + rapZ OKGIIK_02250 0.21 -1.3 527,838 + rapZ OKGIIK_02250 0.21 -2.2 527,838 + rapZ OKGIIK_02250 0.21 -1.3 527,838 + rapZ OKGIIK_02250 0.21 -0.5 527,839 - rapZ OKGIIK_02250 0.21 -0.5 527,839 - rapZ OKGIIK_02250 0.21 -0.8 527,839 - rapZ OKGIIK_02250 0.21 -0.5 527,839 - rapZ OKGIIK_02250 0.21 -1.3 527,839 - rapZ OKGIIK_02250 0.21 -0.5 527,839 - rapZ OKGIIK_02250 0.21 -0.5 527,839 - rapZ OKGIIK_02250 0.21 -1.6 527,839 - rapZ OKGIIK_02250 0.21 -2.2 527,839 - rapZ OKGIIK_02250 0.21 -1.0 527,839 - rapZ OKGIIK_02250 0.21 -0.5 527,839 - rapZ OKGIIK_02250 0.21 -1.0 527,839 - rapZ OKGIIK_02250 0.21 -1.0 527,839 - rapZ OKGIIK_02250 0.21 -0.8 527,839 - rapZ OKGIIK_02250 0.21 -1.0 527,839 - rapZ OKGIIK_02250 0.21 -1.9 527,839 - rapZ OKGIIK_02250 0.21 +0.5 527,839 - rapZ OKGIIK_02250 0.21 -0.8 527,839 - rapZ OKGIIK_02250 0.21 -1.6 527,839 - rapZ OKGIIK_02250 0.21 -0.8 527,839 - rapZ OKGIIK_02250 0.21 -1.8 528,033 + rapZ OKGIIK_02250 0.43 -1.5 528,169 + rapZ OKGIIK_02250 0.58 -0.0 528,170 - rapZ OKGIIK_02250 0.58 -0.8 528,423 + rapZ OKGIIK_02250 0.86 -1.0 528,423 + rapZ OKGIIK_02250 0.86 -0.8 528,423 + rapZ OKGIIK_02250 0.86 +0.3 528,423 + rapZ OKGIIK_02250 0.86 -1.0 528,423 + rapZ OKGIIK_02250 0.86 -1.0 528,423 + rapZ OKGIIK_02250 0.86 -3.8 528,423 + rapZ OKGIIK_02250 0.86 -1.6 528,423 + rapZ OKGIIK_02250 0.86 +0.2 528,424 - rapZ OKGIIK_02250 0.86 -1.0 528,424 - rapZ OKGIIK_02250 0.86 -0.5 528,424 - rapZ OKGIIK_02250 0.86 -1.6 528,424 - rapZ OKGIIK_02250 0.86 -1.6 528,424 - rapZ OKGIIK_02250 0.86 -0.8 528,424 - rapZ OKGIIK_02250 0.86 -1.8 528,424 - rapZ OKGIIK_02250 0.86 +0.2 528,424 - rapZ OKGIIK_02250 0.86 -0.0 528,424 - rapZ OKGIIK_02250 0.86 -1.5 528,424 - rapZ OKGIIK_02250 0.86 -1.0 528,424 - rapZ OKGIIK_02250 0.86 -1.6 528,424 - rapZ OKGIIK_02250 0.86 -1.0 528,424 - rapZ OKGIIK_02250 0.86 -0.8 528,424 - rapZ OKGIIK_02250 0.86 +1.1 528,424 - rapZ OKGIIK_02250 0.86 -1.3 528,424 - rapZ OKGIIK_02250 0.86 -1.3 528,424 - rapZ OKGIIK_02250 0.86 -1.1 528,424 - rapZ OKGIIK_02250 0.86 -0.2 528,458 + rapZ OKGIIK_02250 0.90 -0.9
Or see this region's nucleotide sequence