Experiment: NF
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MPMX19_04879 and MPMX19_04880 are separated by 127 nucleotides MPMX19_04880 and MPMX19_04881 overlap by 4 nucleotides MPMX19_04881 and MPMX19_04882 overlap by 4 nucleotides
MPMX19_04879: MPMX19_04879 - hypothetical protein, at 323,548 to 323,670
_04879
MPMX19_04880: MPMX19_04880 - Inner membrane ABC transporter permease protein YdcV, at 323,798 to 324,631
_04880
MPMX19_04881: MPMX19_04881 - Putrescine transport system permease protein PotH, at 324,628 to 325,572
_04881
MPMX19_04882: MPMX19_04882 - Spermidine/putrescine import ATP-binding protein PotA, at 325,569 to 326,732
_04882
Position (kb)
324
325
326 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 323.708 kb on + strand at 323.708 kb on + strand at 323.708 kb on + strand at 323.708 kb on + strand at 323.709 kb on - strand at 323.709 kb on - strand at 323.709 kb on - strand at 323.709 kb on - strand at 323.709 kb on - strand at 323.709 kb on - strand at 323.709 kb on - strand at 323.768 kb on + strand at 323.768 kb on + strand at 323.768 kb on + strand at 323.768 kb on + strand at 323.768 kb on + strand at 323.768 kb on + strand at 323.768 kb on + strand at 323.768 kb on + strand at 323.768 kb on + strand at 323.768 kb on + strand at 323.768 kb on + strand at 323.769 kb on - strand at 323.769 kb on - strand at 323.769 kb on - strand at 323.769 kb on - strand at 323.769 kb on - strand at 324.186 kb on + strand, within MPMX19_04880 at 324.186 kb on + strand, within MPMX19_04880 at 324.186 kb on + strand, within MPMX19_04880 at 324.186 kb on + strand, within MPMX19_04880 at 324.186 kb on + strand, within MPMX19_04880 at 324.186 kb on + strand, within MPMX19_04880 at 324.186 kb on + strand, within MPMX19_04880 at 324.186 kb on + strand, within MPMX19_04880 at 324.186 kb on + strand, within MPMX19_04880 at 324.186 kb on + strand, within MPMX19_04880 at 324.186 kb on + strand, within MPMX19_04880 at 324.187 kb on - strand, within MPMX19_04880 at 324.187 kb on - strand, within MPMX19_04880 at 324.187 kb on - strand, within MPMX19_04880 at 324.187 kb on - strand, within MPMX19_04880 at 324.187 kb on - strand, within MPMX19_04880 at 324.187 kb on - strand, within MPMX19_04880 at 324.187 kb on - strand, within MPMX19_04880 at 324.187 kb on - strand, within MPMX19_04880 at 324.187 kb on - strand, within MPMX19_04880 at 324.187 kb on - strand, within MPMX19_04880 at 324.187 kb on - strand, within MPMX19_04880 at 324.187 kb on - strand, within MPMX19_04880 at 324.492 kb on + strand, within MPMX19_04880 at 324.492 kb on + strand, within MPMX19_04880 at 324.493 kb on - strand, within MPMX19_04880 at 324.493 kb on - strand, within MPMX19_04880 at 324.493 kb on - strand, within MPMX19_04880 at 324.493 kb on - strand, within MPMX19_04880 at 324.493 kb on - strand, within MPMX19_04880 at 324.576 kb on + strand at 324.576 kb on + strand at 324.576 kb on + strand at 324.576 kb on + strand at 324.576 kb on + strand at 324.576 kb on + strand at 324.577 kb on - strand at 324.577 kb on - strand at 324.577 kb on - strand at 324.577 kb on - strand at 324.577 kb on - strand at 324.577 kb on - strand at 324.577 kb on - strand at 324.577 kb on - strand at 324.577 kb on - strand at 324.577 kb on - strand at 324.589 kb on - strand at 324.589 kb on - strand at 324.589 kb on - strand at 324.998 kb on + strand, within MPMX19_04881 at 324.998 kb on + strand, within MPMX19_04881 at 324.998 kb on + strand, within MPMX19_04881 at 324.998 kb on + strand, within MPMX19_04881 at 324.998 kb on + strand, within MPMX19_04881 at 324.998 kb on + strand, within MPMX19_04881 at 324.998 kb on + strand, within MPMX19_04881 at 324.999 kb on - strand, within MPMX19_04881 at 324.999 kb on - strand, within MPMX19_04881 at 324.999 kb on - strand, within MPMX19_04881 at 324.999 kb on - strand, within MPMX19_04881 at 324.999 kb on - strand, within MPMX19_04881 at 324.999 kb on - strand, within MPMX19_04881 at 324.999 kb on - strand, within MPMX19_04881 at 325.004 kb on + strand, within MPMX19_04881 at 325.004 kb on + strand, within MPMX19_04881 at 325.004 kb on + strand, within MPMX19_04881 at 325.004 kb on + strand, within MPMX19_04881 at 325.004 kb on + strand, within MPMX19_04881 at 325.004 kb on + strand, within MPMX19_04881 at 325.004 kb on + strand, within MPMX19_04881 at 325.004 kb on + strand, within MPMX19_04881 at 325.004 kb on + strand, within MPMX19_04881 at 325.004 kb on + strand, within MPMX19_04881 at 325.004 kb on + strand, within MPMX19_04881 at 325.004 kb on + strand, within MPMX19_04881 at 325.004 kb on + strand, within MPMX19_04881 at 325.004 kb on + strand, within MPMX19_04881 at 325.004 kb on + strand, within MPMX19_04881 at 325.004 kb on + strand, within MPMX19_04881 at 325.004 kb on + strand, within MPMX19_04881 at 325.004 kb on + strand, within MPMX19_04881 at 325.004 kb on + strand, within MPMX19_04881 at 325.004 kb on + strand, within MPMX19_04881 at 325.004 kb on + strand, within MPMX19_04881 at 325.004 kb on + strand, within MPMX19_04881 at 325.005 kb on - strand, within MPMX19_04881 at 325.005 kb on - strand, within MPMX19_04881 at 325.005 kb on - strand, within MPMX19_04881 at 325.005 kb on - strand, within MPMX19_04881 at 325.005 kb on - strand, within MPMX19_04881 at 325.005 kb on - strand, within MPMX19_04881 at 325.005 kb on - strand, within MPMX19_04881 at 325.005 kb on - strand, within MPMX19_04881 at 325.005 kb on - strand, within MPMX19_04881 at 325.005 kb on - strand, within MPMX19_04881 at 325.005 kb on - strand, within MPMX19_04881 at 325.005 kb on - strand, within MPMX19_04881 at 325.005 kb on - strand, within MPMX19_04881 at 325.005 kb on - strand, within MPMX19_04881 at 325.019 kb on + strand, within MPMX19_04881 at 325.019 kb on + strand, within MPMX19_04881 at 325.019 kb on + strand, within MPMX19_04881 at 325.019 kb on + strand, within MPMX19_04881 at 325.020 kb on - strand, within MPMX19_04881 at 325.020 kb on - strand, within MPMX19_04881 at 325.020 kb on - strand, within MPMX19_04881 at 325.020 kb on - strand, within MPMX19_04881 at 325.038 kb on - strand, within MPMX19_04881 at 325.127 kb on + strand, within MPMX19_04881 at 325.127 kb on + strand, within MPMX19_04881 at 325.127 kb on + strand, within MPMX19_04881 at 325.127 kb on + strand, within MPMX19_04881 at 325.127 kb on + strand, within MPMX19_04881 at 325.128 kb on - strand, within MPMX19_04881 at 325.128 kb on - strand, within MPMX19_04881 at 325.128 kb on - strand, within MPMX19_04881 at 325.128 kb on - strand, within MPMX19_04881 at 325.128 kb on - strand, within MPMX19_04881 at 325.128 kb on - strand, within MPMX19_04881 at 325.128 kb on - strand, within MPMX19_04881 at 325.128 kb on - strand, within MPMX19_04881 at 325.217 kb on + strand, within MPMX19_04881 at 325.217 kb on + strand, within MPMX19_04881 at 325.217 kb on + strand, within MPMX19_04881 at 325.217 kb on + strand, within MPMX19_04881 at 325.217 kb on + strand, within MPMX19_04881 at 325.217 kb on + strand, within MPMX19_04881 at 325.217 kb on + strand, within MPMX19_04881 at 325.217 kb on + strand, within MPMX19_04881 at 325.218 kb on - strand, within MPMX19_04881 at 325.218 kb on - strand, within MPMX19_04881 at 325.218 kb on - strand, within MPMX19_04881 at 325.218 kb on - strand, within MPMX19_04881 at 325.286 kb on + strand, within MPMX19_04881 at 325.286 kb on + strand, within MPMX19_04881 at 325.298 kb on + strand, within MPMX19_04881 at 325.298 kb on + strand, within MPMX19_04881 at 325.298 kb on + strand, within MPMX19_04881 at 325.298 kb on + strand, within MPMX19_04881 at 325.299 kb on - strand, within MPMX19_04881 at 325.299 kb on - strand, within MPMX19_04881 at 325.299 kb on - strand, within MPMX19_04881 at 325.299 kb on - strand, within MPMX19_04881 at 325.299 kb on - strand, within MPMX19_04881 at 325.299 kb on - strand, within MPMX19_04881 at 325.299 kb on - strand, within MPMX19_04881 at 325.299 kb on - strand, within MPMX19_04881 at 325.299 kb on - strand, within MPMX19_04881 at 325.299 kb on - strand, within MPMX19_04881 at 325.299 kb on - strand, within MPMX19_04881 at 325.299 kb on - strand, within MPMX19_04881 at 325.325 kb on + strand, within MPMX19_04881 at 325.325 kb on + strand, within MPMX19_04881 at 325.325 kb on + strand, within MPMX19_04881 at 325.325 kb on + strand, within MPMX19_04881 at 325.326 kb on - strand, within MPMX19_04881 at 325.326 kb on - strand, within MPMX19_04881 at 325.404 kb on - strand, within MPMX19_04881 at 325.490 kb on + strand at 325.490 kb on + strand at 325.490 kb on + strand at 325.491 kb on - strand at 325.491 kb on - strand at 325.491 kb on - strand at 325.491 kb on - strand at 325.491 kb on - strand at 325.708 kb on + strand, within MPMX19_04882 at 325.708 kb on + strand, within MPMX19_04882 at 325.708 kb on + strand, within MPMX19_04882 at 325.708 kb on + strand, within MPMX19_04882 at 325.708 kb on + strand, within MPMX19_04882 at 325.709 kb on - strand, within MPMX19_04882 at 325.709 kb on - strand, within MPMX19_04882 at 325.709 kb on - strand, within MPMX19_04882 at 325.709 kb on - strand, within MPMX19_04882 at 325.730 kb on - strand, within MPMX19_04882 at 326.008 kb on + strand, within MPMX19_04882 at 326.008 kb on + strand, within MPMX19_04882 at 326.008 kb on + strand, within MPMX19_04882 at 326.008 kb on + strand, within MPMX19_04882 at 326.009 kb on - strand, within MPMX19_04882 at 326.009 kb on - strand, within MPMX19_04882 at 326.009 kb on - strand, within MPMX19_04882 at 326.422 kb on + strand, within MPMX19_04882 at 326.422 kb on + strand, within MPMX19_04882 at 326.423 kb on - strand, within MPMX19_04882 at 326.423 kb on - strand, within MPMX19_04882 at 326.455 kb on + strand, within MPMX19_04882 at 326.455 kb on + strand, within MPMX19_04882 at 326.456 kb on - strand, within MPMX19_04882 at 326.456 kb on - strand, within MPMX19_04882 at 326.491 kb on + strand, within MPMX19_04882 at 326.491 kb on + strand, within MPMX19_04882 at 326.491 kb on + strand, within MPMX19_04882 at 326.491 kb on + strand, within MPMX19_04882 at 326.491 kb on + strand, within MPMX19_04882 at 326.491 kb on + strand, within MPMX19_04882 at 326.491 kb on + strand, within MPMX19_04882 at 326.491 kb on + strand, within MPMX19_04882 at 326.491 kb on + strand, within MPMX19_04882 at 326.491 kb on + strand, within MPMX19_04882 at 326.491 kb on + strand, within MPMX19_04882 at 326.491 kb on + strand, within MPMX19_04882 at 326.491 kb on + strand, within MPMX19_04882 at 326.491 kb on + strand, within MPMX19_04882 at 326.491 kb on + strand, within MPMX19_04882 at 326.492 kb on - strand, within MPMX19_04882 at 326.492 kb on - strand, within MPMX19_04882 at 326.492 kb on - strand, within MPMX19_04882 at 326.492 kb on - strand, within MPMX19_04882 at 326.492 kb on - strand, within MPMX19_04882 at 326.492 kb on - strand, within MPMX19_04882
Per-strain Table
Position Strand Gene LocusTag Fraction NF remove 323,708 + -1.4 323,708 + -0.6 323,708 + -1.1 323,708 + -0.5 323,709 - -1.3 323,709 - -0.9 323,709 - +0.6 323,709 - -1.2 323,709 - -1.4 323,709 - -0.4 323,709 - -0.1 323,768 + +0.4 323,768 + -1.4 323,768 + +0.2 323,768 + -0.6 323,768 + +0.7 323,768 + -0.6 323,768 + +0.1 323,768 + +0.2 323,768 + -0.1 323,768 + -0.1 323,768 + +1.2 323,769 - +0.5 323,769 - -0.4 323,769 - +0.0 323,769 - -1.3 323,769 - -1.4 324,186 + MPMX19_04880 0.47 +0.1 324,186 + MPMX19_04880 0.47 +0.9 324,186 + MPMX19_04880 0.47 +0.4 324,186 + MPMX19_04880 0.47 -1.4 324,186 + MPMX19_04880 0.47 +0.1 324,186 + MPMX19_04880 0.47 +0.1 324,186 + MPMX19_04880 0.47 +0.7 324,186 + MPMX19_04880 0.47 +0.6 324,186 + MPMX19_04880 0.47 -0.1 324,186 + MPMX19_04880 0.47 -1.6 324,186 + MPMX19_04880 0.47 -0.2 324,187 - MPMX19_04880 0.47 -0.4 324,187 - MPMX19_04880 0.47 -1.4 324,187 - MPMX19_04880 0.47 -0.2 324,187 - MPMX19_04880 0.47 -0.1 324,187 - MPMX19_04880 0.47 +0.4 324,187 - MPMX19_04880 0.47 -0.7 324,187 - MPMX19_04880 0.47 -1.5 324,187 - MPMX19_04880 0.47 +0.8 324,187 - MPMX19_04880 0.47 -0.1 324,187 - MPMX19_04880 0.47 +0.1 324,187 - MPMX19_04880 0.47 +1.2 324,187 - MPMX19_04880 0.47 +0.2 324,492 + MPMX19_04880 0.83 +0.9 324,492 + MPMX19_04880 0.83 +0.2 324,493 - MPMX19_04880 0.83 -1.1 324,493 - MPMX19_04880 0.83 -0.8 324,493 - MPMX19_04880 0.83 -2.6 324,493 - MPMX19_04880 0.83 +1.8 324,493 - MPMX19_04880 0.83 +1.5 324,576 + +0.9 324,576 + +1.2 324,576 + +0.1 324,576 + -1.1 324,576 + +0.4 324,576 + +0.1 324,577 - +0.4 324,577 - -0.6 324,577 - -0.2 324,577 - +0.4 324,577 - +1.1 324,577 - -0.3 324,577 - -0.6 324,577 - -1.4 324,577 - +1.9 324,577 - -2.0 324,589 - -1.5 324,589 - -0.9 324,589 - -1.0 324,998 + MPMX19_04881 0.39 +0.5 324,998 + MPMX19_04881 0.39 +0.6 324,998 + MPMX19_04881 0.39 -0.1 324,998 + MPMX19_04881 0.39 -0.9 324,998 + MPMX19_04881 0.39 -0.5 324,998 + MPMX19_04881 0.39 -0.9 324,998 + MPMX19_04881 0.39 -1.0 324,999 - MPMX19_04881 0.39 +0.9 324,999 - MPMX19_04881 0.39 +0.4 324,999 - MPMX19_04881 0.39 -0.6 324,999 - MPMX19_04881 0.39 +0.2 324,999 - MPMX19_04881 0.39 +0.9 324,999 - MPMX19_04881 0.39 -0.4 324,999 - MPMX19_04881 0.39 -0.6 325,004 + MPMX19_04881 0.40 +0.5 325,004 + MPMX19_04881 0.40 -0.2 325,004 + MPMX19_04881 0.40 -0.4 325,004 + MPMX19_04881 0.40 -2.2 325,004 + MPMX19_04881 0.40 -1.6 325,004 + MPMX19_04881 0.40 -1.0 325,004 + MPMX19_04881 0.40 +0.5 325,004 + MPMX19_04881 0.40 +1.5 325,004 + MPMX19_04881 0.40 -0.1 325,004 + MPMX19_04881 0.40 +0.5 325,004 + MPMX19_04881 0.40 -0.1 325,004 + MPMX19_04881 0.40 -1.2 325,004 + MPMX19_04881 0.40 -0.2 325,004 + MPMX19_04881 0.40 -0.4 325,004 + MPMX19_04881 0.40 -1.2 325,004 + MPMX19_04881 0.40 -0.1 325,004 + MPMX19_04881 0.40 +0.6 325,004 + MPMX19_04881 0.40 -0.6 325,004 + MPMX19_04881 0.40 -0.1 325,004 + MPMX19_04881 0.40 -0.6 325,004 + MPMX19_04881 0.40 -0.6 325,004 + MPMX19_04881 0.40 -0.4 325,005 - MPMX19_04881 0.40 +0.4 325,005 - MPMX19_04881 0.40 +0.4 325,005 - MPMX19_04881 0.40 -0.2 325,005 - MPMX19_04881 0.40 -0.5 325,005 - MPMX19_04881 0.40 -0.9 325,005 - MPMX19_04881 0.40 -0.1 325,005 - MPMX19_04881 0.40 -1.5 325,005 - MPMX19_04881 0.40 -0.4 325,005 - MPMX19_04881 0.40 -0.6 325,005 - MPMX19_04881 0.40 -1.3 325,005 - MPMX19_04881 0.40 -0.9 325,005 - MPMX19_04881 0.40 -0.6 325,005 - MPMX19_04881 0.40 -0.1 325,005 - MPMX19_04881 0.40 -0.9 325,019 + MPMX19_04881 0.41 -1.8 325,019 + MPMX19_04881 0.41 -0.9 325,019 + MPMX19_04881 0.41 +0.4 325,019 + MPMX19_04881 0.41 -1.8 325,020 - MPMX19_04881 0.41 -1.4 325,020 - MPMX19_04881 0.41 -1.4 325,020 - MPMX19_04881 0.41 -0.9 325,020 - MPMX19_04881 0.41 -0.1 325,038 - MPMX19_04881 0.43 -1.9 325,127 + MPMX19_04881 0.53 +0.1 325,127 + MPMX19_04881 0.53 +1.9 325,127 + MPMX19_04881 0.53 -2.1 325,127 + MPMX19_04881 0.53 +0.9 325,127 + MPMX19_04881 0.53 +1.7 325,128 - MPMX19_04881 0.53 -0.9 325,128 - MPMX19_04881 0.53 +0.6 325,128 - MPMX19_04881 0.53 -0.3 325,128 - MPMX19_04881 0.53 -0.4 325,128 - MPMX19_04881 0.53 -0.3 325,128 - MPMX19_04881 0.53 +0.5 325,128 - MPMX19_04881 0.53 +0.6 325,128 - MPMX19_04881 0.53 -0.4 325,217 + MPMX19_04881 0.62 -0.6 325,217 + MPMX19_04881 0.62 +2.6 325,217 + MPMX19_04881 0.62 -1.7 325,217 + MPMX19_04881 0.62 +0.9 325,217 + MPMX19_04881 0.62 -0.1 325,217 + MPMX19_04881 0.62 -0.9 325,217 + MPMX19_04881 0.62 -1.2 325,217 + MPMX19_04881 0.62 +0.4 325,218 - MPMX19_04881 0.62 -0.7 325,218 - MPMX19_04881 0.62 -0.4 325,218 - MPMX19_04881 0.62 -1.4 325,218 - MPMX19_04881 0.62 -2.0 325,286 + MPMX19_04881 0.70 -0.9 325,286 + MPMX19_04881 0.70 +0.7 325,298 + MPMX19_04881 0.71 -1.1 325,298 + MPMX19_04881 0.71 +0.1 325,298 + MPMX19_04881 0.71 -0.2 325,298 + MPMX19_04881 0.71 -0.8 325,299 - MPMX19_04881 0.71 -0.9 325,299 - MPMX19_04881 0.71 +0.7 325,299 - MPMX19_04881 0.71 -0.6 325,299 - MPMX19_04881 0.71 +1.8 325,299 - MPMX19_04881 0.71 +0.8 325,299 - MPMX19_04881 0.71 +0.4 325,299 - MPMX19_04881 0.71 -0.9 325,299 - MPMX19_04881 0.71 +0.5 325,299 - MPMX19_04881 0.71 -1.6 325,299 - MPMX19_04881 0.71 +0.4 325,299 - MPMX19_04881 0.71 -0.6 325,299 - MPMX19_04881 0.71 -0.9 325,325 + MPMX19_04881 0.74 -0.6 325,325 + MPMX19_04881 0.74 -1.0 325,325 + MPMX19_04881 0.74 +0.6 325,325 + MPMX19_04881 0.74 -0.2 325,326 - MPMX19_04881 0.74 -0.5 325,326 - MPMX19_04881 0.74 +0.5 325,404 - MPMX19_04881 0.82 +0.9 325,490 + +0.4 325,490 + -0.2 325,490 + +0.4 325,491 - -0.3 325,491 - +1.4 325,491 - -0.5 325,491 - +0.4 325,491 - -0.1 325,708 + MPMX19_04882 0.12 -0.6 325,708 + MPMX19_04882 0.12 +0.6 325,708 + MPMX19_04882 0.12 +0.5 325,708 + MPMX19_04882 0.12 -0.5 325,708 + MPMX19_04882 0.12 -2.4 325,709 - MPMX19_04882 0.12 -1.1 325,709 - MPMX19_04882 0.12 +0.8 325,709 - MPMX19_04882 0.12 +1.9 325,709 - MPMX19_04882 0.12 -0.1 325,730 - MPMX19_04882 0.14 -1.2 326,008 + MPMX19_04882 0.38 -0.6 326,008 + MPMX19_04882 0.38 +0.9 326,008 + MPMX19_04882 0.38 -0.6 326,008 + MPMX19_04882 0.38 -1.1 326,009 - MPMX19_04882 0.38 +1.6 326,009 - MPMX19_04882 0.38 -0.6 326,009 - MPMX19_04882 0.38 -1.4 326,422 + MPMX19_04882 0.73 +0.8 326,422 + MPMX19_04882 0.73 -1.3 326,423 - MPMX19_04882 0.73 +1.1 326,423 - MPMX19_04882 0.73 -0.1 326,455 + MPMX19_04882 0.76 +0.9 326,455 + MPMX19_04882 0.76 +1.2 326,456 - MPMX19_04882 0.76 -0.4 326,456 - MPMX19_04882 0.76 -1.2 326,491 + MPMX19_04882 0.79 -1.5 326,491 + MPMX19_04882 0.79 +0.5 326,491 + MPMX19_04882 0.79 +0.7 326,491 + MPMX19_04882 0.79 +0.1 326,491 + MPMX19_04882 0.79 -1.2 326,491 + MPMX19_04882 0.79 +0.9 326,491 + MPMX19_04882 0.79 -0.6 326,491 + MPMX19_04882 0.79 +0.7 326,491 + MPMX19_04882 0.79 +0.8 326,491 + MPMX19_04882 0.79 -0.9 326,491 + MPMX19_04882 0.79 +0.3 326,491 + MPMX19_04882 0.79 -2.5 326,491 + MPMX19_04882 0.79 -1.1 326,491 + MPMX19_04882 0.79 +0.9 326,491 + MPMX19_04882 0.79 -1.2 326,492 - MPMX19_04882 0.79 -1.1 326,492 - MPMX19_04882 0.79 -1.2 326,492 - MPMX19_04882 0.79 -0.5 326,492 - MPMX19_04882 0.79 +0.9 326,492 - MPMX19_04882 0.79 -1.4 326,492 - MPMX19_04882 0.79 +1.4
Or see this region's nucleotide sequence