Experiment: Mixed culture; Methylosinus sp. DMC45 1:1 with Pseudomonas_DMC3_ML3; dNMS with methane
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4244 and GFF4245 are separated by 84 nucleotides GFF4245 and GFF4246 are separated by 88 nucleotides GFF4246 and GFF4247 are separated by 3 nucleotides GFF4247 and GFF4248 are separated by 12 nucleotides
GFF4244 - CRP-like cAMP-activated global transcriptional regulator, at 218,682 to 219,359
GFF4244
GFF4245 - hypothetical protein, at 219,444 to 219,971
GFF4245
GFF4246 - Sulfate/thiosulfate import ATP-binding protein CysA, at 220,060 to 221,049
GFF4246
GFF4247 - Sulfate transport system permease protein CysW, at 221,053 to 221,925
GFF4247
GFF4248 - Sulfate transport system permease protein CysT, at 221,938 to 222,756
GFF4248
Position (kb) 220
221
222 Strain fitness (log2 ratio) -2
-1
0
1
2 at 219.073 kb on + strand, within GFF4244 at 219.074 kb on - strand, within GFF4244 at 219.074 kb on - strand, within GFF4244 at 219.074 kb on - strand, within GFF4244 at 219.474 kb on + strand at 219.595 kb on + strand, within GFF4245 at 219.595 kb on + strand, within GFF4245 at 219.596 kb on - strand, within GFF4245 at 219.596 kb on - strand, within GFF4245 at 219.637 kb on - strand, within GFF4245 at 219.637 kb on - strand, within GFF4245 at 219.637 kb on - strand, within GFF4245 at 219.676 kb on + strand, within GFF4245 at 219.798 kb on + strand, within GFF4245 at 219.798 kb on + strand, within GFF4245 at 219.798 kb on + strand, within GFF4245 at 219.798 kb on + strand, within GFF4245 at 219.798 kb on + strand, within GFF4245 at 219.798 kb on + strand, within GFF4245 at 219.798 kb on + strand, within GFF4245 at 220.006 kb on - strand at 220.061 kb on + strand at 220.276 kb on + strand, within GFF4246 at 220.276 kb on + strand, within GFF4246 at 220.276 kb on + strand, within GFF4246 at 220.276 kb on + strand, within GFF4246 at 220.276 kb on + strand, within GFF4246 at 220.276 kb on + strand, within GFF4246 at 220.276 kb on + strand, within GFF4246 at 220.277 kb on - strand, within GFF4246 at 220.277 kb on - strand, within GFF4246 at 220.277 kb on - strand, within GFF4246 at 220.277 kb on - strand, within GFF4246 at 220.350 kb on - strand, within GFF4246 at 220.494 kb on + strand, within GFF4246 at 220.495 kb on - strand, within GFF4246 at 220.495 kb on - strand, within GFF4246 at 220.495 kb on - strand, within GFF4246 at 220.495 kb on - strand, within GFF4246 at 220.495 kb on - strand, within GFF4246 at 220.617 kb on + strand, within GFF4246 at 220.802 kb on + strand, within GFF4246 at 220.803 kb on - strand, within GFF4246 at 220.827 kb on + strand, within GFF4246 at 220.827 kb on + strand, within GFF4246 at 220.827 kb on + strand, within GFF4246 at 220.908 kb on + strand, within GFF4246 at 220.909 kb on - strand, within GFF4246 at 221.054 kb on + strand at 221.054 kb on + strand at 221.054 kb on + strand at 221.055 kb on - strand at 221.177 kb on + strand, within GFF4247 at 221.177 kb on + strand, within GFF4247 at 221.177 kb on + strand, within GFF4247 at 221.177 kb on + strand, within GFF4247 at 221.178 kb on - strand, within GFF4247 at 221.186 kb on + strand, within GFF4247 at 221.186 kb on + strand, within GFF4247 at 221.187 kb on - strand, within GFF4247 at 221.553 kb on - strand, within GFF4247 at 221.633 kb on + strand, within GFF4247 at 221.633 kb on + strand, within GFF4247 at 221.634 kb on - strand, within GFF4247 at 221.669 kb on - strand, within GFF4247 at 221.939 kb on + strand at 221.940 kb on - strand at 222.010 kb on + strand at 222.011 kb on - strand at 222.035 kb on + strand, within GFF4248 at 222.035 kb on + strand, within GFF4248 at 222.035 kb on + strand, within GFF4248 at 222.035 kb on + strand, within GFF4248 at 222.036 kb on - strand, within GFF4248 at 222.036 kb on - strand, within GFF4248
Per-strain Table
Position Strand Gene LocusTag Fraction Mixed culture; Methylosinus sp. DMC45 1:1 with Pseudomonas_DMC3_ML3; dNMS with methane remove 219,073 + GFF4244 0.58 +0.1 219,074 - GFF4244 0.58 +0.8 219,074 - GFF4244 0.58 -0.2 219,074 - GFF4244 0.58 -1.2 219,474 + -2.0 219,595 + GFF4245 0.29 -0.6 219,595 + GFF4245 0.29 +1.0 219,596 - GFF4245 0.29 -0.4 219,596 - GFF4245 0.29 -0.3 219,637 - GFF4245 0.37 +1.2 219,637 - GFF4245 0.37 -0.0 219,637 - GFF4245 0.37 +0.7 219,676 + GFF4245 0.44 +0.5 219,798 + GFF4245 0.67 -0.7 219,798 + GFF4245 0.67 -0.5 219,798 + GFF4245 0.67 +0.0 219,798 + GFF4245 0.67 +0.2 219,798 + GFF4245 0.67 -0.2 219,798 + GFF4245 0.67 -1.7 219,798 + GFF4245 0.67 +0.3 220,006 - -0.9 220,061 + -0.9 220,276 + GFF4246 0.22 +0.3 220,276 + GFF4246 0.22 -0.4 220,276 + GFF4246 0.22 +0.2 220,276 + GFF4246 0.22 -0.0 220,276 + GFF4246 0.22 +0.9 220,276 + GFF4246 0.22 +1.9 220,276 + GFF4246 0.22 -0.6 220,277 - GFF4246 0.22 +0.1 220,277 - GFF4246 0.22 +0.5 220,277 - GFF4246 0.22 +0.3 220,277 - GFF4246 0.22 +0.5 220,350 - GFF4246 0.29 +0.1 220,494 + GFF4246 0.44 -0.8 220,495 - GFF4246 0.44 +0.4 220,495 - GFF4246 0.44 +1.2 220,495 - GFF4246 0.44 +0.1 220,495 - GFF4246 0.44 -0.5 220,495 - GFF4246 0.44 -0.0 220,617 + GFF4246 0.56 -0.0 220,802 + GFF4246 0.75 -0.3 220,803 - GFF4246 0.75 -0.9 220,827 + GFF4246 0.77 -0.7 220,827 + GFF4246 0.77 -0.2 220,827 + GFF4246 0.77 -0.6 220,908 + GFF4246 0.86 -0.3 220,909 - GFF4246 0.86 -2.7 221,054 + -0.1 221,054 + +0.3 221,054 + +0.3 221,055 - +0.5 221,177 + GFF4247 0.14 -1.3 221,177 + GFF4247 0.14 -0.9 221,177 + GFF4247 0.14 -1.3 221,177 + GFF4247 0.14 +0.3 221,178 - GFF4247 0.14 +0.6 221,186 + GFF4247 0.15 -1.2 221,186 + GFF4247 0.15 +1.0 221,187 - GFF4247 0.15 -0.5 221,553 - GFF4247 0.57 -0.6 221,633 + GFF4247 0.66 -0.2 221,633 + GFF4247 0.66 -2.2 221,634 - GFF4247 0.67 -1.2 221,669 - GFF4247 0.71 -0.1 221,939 + +0.1 221,940 - -0.4 222,010 + -0.3 222,011 - -1.0 222,035 + GFF4248 0.12 +0.3 222,035 + GFF4248 0.12 +0.1 222,035 + GFF4248 0.12 +1.1 222,035 + GFF4248 0.12 +0.9 222,036 - GFF4248 0.12 -0.4 222,036 - GFF4248 0.12 +1.4
Or see this region's nucleotide sequence