Experiment: Mixed culture; Methylosinus sp. DMC45 1:1 with Pseudomonas_DMC3_ML3; dNMS with methane
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF20 and GFF21 are separated by 70 nucleotides GFF21 and GFF22 overlap by 4 nucleotides GFF22 and GFF23 are separated by 114 nucleotides
GFF20 - Anthranilate synthase component 1, at 18,780 to 20,261
GFF20
GFF21 - Phosphoglycolate phosphatase, at 20,332 to 21,150
GFF21
GFF22 - Ribulose-phosphate 3-epimerase, at 21,147 to 21,821
GFF22
GFF23 - Long-chain-alcohol dehydrogenase 1, at 21,936 to 23,084
GFF23
Position (kb) 21
22 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 20.161 kb on + strand at 20.162 kb on - strand at 20.222 kb on - strand at 20.222 kb on - strand at 20.261 kb on + strand at 20.261 kb on + strand at 20.262 kb on - strand at 20.297 kb on + strand at 20.297 kb on + strand at 20.298 kb on - strand at 20.476 kb on + strand, within GFF21 at 20.477 kb on - strand, within GFF21 at 20.540 kb on + strand, within GFF21 at 20.541 kb on - strand, within GFF21 at 20.546 kb on + strand, within GFF21 at 20.547 kb on - strand, within GFF21 at 20.547 kb on - strand, within GFF21 at 20.723 kb on + strand, within GFF21 at 20.723 kb on + strand, within GFF21 at 20.724 kb on - strand, within GFF21 at 20.724 kb on - strand, within GFF21 at 20.724 kb on - strand, within GFF21 at 20.844 kb on - strand, within GFF21 at 21.841 kb on - strand at 21.841 kb on - strand at 21.862 kb on + strand at 21.862 kb on + strand at 21.862 kb on + strand at 21.862 kb on + strand at 21.862 kb on + strand at 21.862 kb on + strand at 21.862 kb on + strand at 21.862 kb on + strand at 21.863 kb on - strand at 21.863 kb on - strand at 21.863 kb on - strand at 21.863 kb on - strand at 21.863 kb on - strand at 21.863 kb on - strand at 21.863 kb on - strand at 21.863 kb on - strand at 21.863 kb on - strand at 21.863 kb on - strand at 21.863 kb on - strand at 21.863 kb on - strand at 21.864 kb on + strand at 21.865 kb on - strand at 22.002 kb on + strand at 22.231 kb on + strand, within GFF23 at 22.231 kb on + strand, within GFF23 at 22.232 kb on - strand, within GFF23 at 22.232 kb on - strand, within GFF23 at 22.232 kb on - strand, within GFF23 at 22.232 kb on - strand, within GFF23 at 22.232 kb on - strand, within GFF23 at 22.232 kb on - strand, within GFF23 at 22.232 kb on - strand, within GFF23 at 22.399 kb on + strand, within GFF23 at 22.483 kb on + strand, within GFF23 at 22.483 kb on + strand, within GFF23 at 22.484 kb on - strand, within GFF23 at 22.484 kb on - strand, within GFF23 at 22.591 kb on + strand, within GFF23 at 22.591 kb on + strand, within GFF23 at 22.592 kb on - strand, within GFF23
Per-strain Table
Position Strand Gene LocusTag Fraction Mixed culture; Methylosinus sp. DMC45 1:1 with Pseudomonas_DMC3_ML3; dNMS with methane remove 20,161 + -0.7 20,162 - -3.6 20,222 - -2.0 20,222 - -3.2 20,261 + -2.3 20,261 + -1.6 20,262 - -0.5 20,297 + -1.0 20,297 + -1.5 20,298 - -0.4 20,476 + GFF21 0.18 -0.9 20,477 - GFF21 0.18 -0.9 20,540 + GFF21 0.25 +1.2 20,541 - GFF21 0.26 -0.7 20,546 + GFF21 0.26 +1.2 20,547 - GFF21 0.26 +0.3 20,547 - GFF21 0.26 +0.1 20,723 + GFF21 0.48 -0.3 20,723 + GFF21 0.48 -0.4 20,724 - GFF21 0.48 -0.1 20,724 - GFF21 0.48 -3.5 20,724 - GFF21 0.48 -0.7 20,844 - GFF21 0.63 +0.2 21,841 - -0.1 21,841 - -1.9 21,862 + -0.0 21,862 + +0.8 21,862 + +0.4 21,862 + +0.7 21,862 + -1.6 21,862 + -1.0 21,862 + -1.8 21,862 + -1.7 21,863 - +1.0 21,863 - -0.2 21,863 - +0.3 21,863 - -0.6 21,863 - +0.9 21,863 - -0.3 21,863 - +0.2 21,863 - -0.4 21,863 - -2.0 21,863 - +0.0 21,863 - -0.1 21,863 - +0.2 21,864 + +0.1 21,865 - -0.3 22,002 + +0.4 22,231 + GFF23 0.26 +0.9 22,231 + GFF23 0.26 -2.2 22,232 - GFF23 0.26 -0.9 22,232 - GFF23 0.26 -0.3 22,232 - GFF23 0.26 -1.2 22,232 - GFF23 0.26 -1.2 22,232 - GFF23 0.26 -0.2 22,232 - GFF23 0.26 -0.3 22,232 - GFF23 0.26 -1.0 22,399 + GFF23 0.40 -1.1 22,483 + GFF23 0.48 -1.1 22,483 + GFF23 0.48 +0.9 22,484 - GFF23 0.48 -1.2 22,484 - GFF23 0.48 -0.9 22,591 + GFF23 0.57 -0.5 22,591 + GFF23 0.57 +0.2 22,592 - GFF23 0.57 +0.3
Or see this region's nucleotide sequence