Experiment: Mixed culture; Methylosinus sp. DMC45 1:1 with Pseudomonas_DMC3_ML3; dNMS with methane
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2080 and GFF2081 overlap by 13 nucleotides GFF2081 and GFF2082 overlap by 1 nucleotides GFF2082 and GFF2083 are separated by 210 nucleotides
GFF2080 - tRNA pseudouridine synthase D, at 329,607 to 330,665
GFF2080
GFF2081 - 5'-nucleotidase SurE, at 330,653 to 331,402
GFF2081
GFF2082 - Protein-L-isoaspartate O-methyltransferase, at 331,402 to 332,076
GFF2082
GFF2083 - Murein hydrolase activator NlpD, at 332,287 to 333,156
GFF2083
Position (kb) 331
332
333 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 330.602 kb on - strand at 330.602 kb on - strand at 330.658 kb on + strand at 330.659 kb on - strand at 330.659 kb on - strand at 330.659 kb on - strand at 330.670 kb on + strand at 330.671 kb on - strand at 330.711 kb on - strand at 330.711 kb on - strand at 330.711 kb on - strand at 330.711 kb on - strand at 330.711 kb on - strand at 330.729 kb on - strand, within GFF2081 at 330.815 kb on + strand, within GFF2081 at 330.957 kb on - strand, within GFF2081 at 330.957 kb on - strand, within GFF2081 at 331.061 kb on + strand, within GFF2081 at 331.061 kb on + strand, within GFF2081 at 331.119 kb on + strand, within GFF2081 at 331.119 kb on + strand, within GFF2081 at 331.123 kb on + strand, within GFF2081 at 331.123 kb on + strand, within GFF2081 at 331.123 kb on + strand, within GFF2081 at 331.124 kb on - strand, within GFF2081 at 331.124 kb on - strand, within GFF2081 at 331.124 kb on - strand, within GFF2081 at 331.124 kb on - strand, within GFF2081 at 331.316 kb on + strand, within GFF2081 at 331.316 kb on + strand, within GFF2081 at 331.316 kb on + strand, within GFF2081 at 331.317 kb on - strand, within GFF2081 at 331.317 kb on - strand, within GFF2081 at 331.317 kb on - strand, within GFF2081 at 331.317 kb on - strand, within GFF2081 at 331.483 kb on + strand, within GFF2082 at 331.483 kb on + strand, within GFF2082 at 331.484 kb on - strand, within GFF2082 at 331.515 kb on - strand, within GFF2082 at 331.576 kb on + strand, within GFF2082 at 331.577 kb on - strand, within GFF2082 at 331.577 kb on - strand, within GFF2082 at 331.627 kb on + strand, within GFF2082 at 331.627 kb on + strand, within GFF2082 at 331.627 kb on + strand, within GFF2082 at 331.627 kb on + strand, within GFF2082 at 331.627 kb on + strand, within GFF2082 at 331.627 kb on + strand, within GFF2082 at 331.627 kb on + strand, within GFF2082 at 331.627 kb on + strand, within GFF2082 at 331.627 kb on + strand, within GFF2082 at 331.628 kb on - strand, within GFF2082 at 331.628 kb on - strand, within GFF2082 at 331.628 kb on - strand, within GFF2082 at 331.628 kb on - strand, within GFF2082 at 331.628 kb on - strand, within GFF2082 at 331.628 kb on - strand, within GFF2082 at 331.628 kb on - strand, within GFF2082 at 331.628 kb on - strand, within GFF2082 at 331.871 kb on - strand, within GFF2082 at 331.871 kb on - strand, within GFF2082 at 331.871 kb on - strand, within GFF2082 at 331.907 kb on + strand, within GFF2082 at 331.908 kb on - strand, within GFF2082 at 332.120 kb on + strand at 332.121 kb on - strand at 332.121 kb on - strand at 332.121 kb on - strand at 332.150 kb on + strand at 332.150 kb on + strand at 332.150 kb on + strand at 332.151 kb on - strand at 332.151 kb on - strand at 332.151 kb on - strand at 332.194 kb on + strand at 332.195 kb on - strand at 332.200 kb on + strand at 332.221 kb on + strand at 332.221 kb on + strand at 332.221 kb on + strand at 332.221 kb on + strand at 332.222 kb on - strand at 332.222 kb on - strand at 332.222 kb on - strand at 332.222 kb on - strand at 332.313 kb on + strand at 332.314 kb on - strand at 332.314 kb on - strand at 332.320 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Mixed culture; Methylosinus sp. DMC45 1:1 with Pseudomonas_DMC3_ML3; dNMS with methane remove 330,602 - +1.1 330,602 - +0.0 330,658 + -1.1 330,659 - +0.5 330,659 - -1.6 330,659 - -0.3 330,670 + -0.0 330,671 - +0.3 330,711 - +0.4 330,711 - -0.4 330,711 - -0.1 330,711 - +0.4 330,711 - +0.3 330,729 - GFF2081 0.10 -1.0 330,815 + GFF2081 0.22 -1.0 330,957 - GFF2081 0.41 -0.1 330,957 - GFF2081 0.41 +0.1 331,061 + GFF2081 0.54 -0.5 331,061 + GFF2081 0.54 +1.2 331,119 + GFF2081 0.62 -1.5 331,119 + GFF2081 0.62 -0.7 331,123 + GFF2081 0.63 -0.4 331,123 + GFF2081 0.63 +0.3 331,123 + GFF2081 0.63 +0.2 331,124 - GFF2081 0.63 -1.5 331,124 - GFF2081 0.63 -0.9 331,124 - GFF2081 0.63 -0.0 331,124 - GFF2081 0.63 -0.6 331,316 + GFF2081 0.88 -0.3 331,316 + GFF2081 0.88 -0.8 331,316 + GFF2081 0.88 -2.6 331,317 - GFF2081 0.89 -0.4 331,317 - GFF2081 0.89 +0.7 331,317 - GFF2081 0.89 +1.1 331,317 - GFF2081 0.89 -1.0 331,483 + GFF2082 0.12 +0.7 331,483 + GFF2082 0.12 -0.2 331,484 - GFF2082 0.12 +0.1 331,515 - GFF2082 0.17 -0.2 331,576 + GFF2082 0.26 +0.7 331,577 - GFF2082 0.26 +0.8 331,577 - GFF2082 0.26 +1.1 331,627 + GFF2082 0.33 -0.3 331,627 + GFF2082 0.33 -0.5 331,627 + GFF2082 0.33 +0.1 331,627 + GFF2082 0.33 -2.8 331,627 + GFF2082 0.33 +1.8 331,627 + GFF2082 0.33 +0.1 331,627 + GFF2082 0.33 +1.0 331,627 + GFF2082 0.33 -0.3 331,627 + GFF2082 0.33 +0.8 331,628 - GFF2082 0.33 +1.1 331,628 - GFF2082 0.33 +0.7 331,628 - GFF2082 0.33 -0.3 331,628 - GFF2082 0.33 +0.7 331,628 - GFF2082 0.33 +0.5 331,628 - GFF2082 0.33 -2.0 331,628 - GFF2082 0.33 -0.4 331,628 - GFF2082 0.33 -0.5 331,871 - GFF2082 0.69 -0.3 331,871 - GFF2082 0.69 -0.8 331,871 - GFF2082 0.69 +2.0 331,907 + GFF2082 0.75 +0.3 331,908 - GFF2082 0.75 +0.3 332,120 + -0.8 332,121 - +1.5 332,121 - -0.8 332,121 - -1.0 332,150 + -0.1 332,150 + +1.3 332,150 + -0.5 332,151 - +1.7 332,151 - -0.5 332,151 - -0.1 332,194 + +1.5 332,195 - -0.2 332,200 + +2.2 332,221 + -0.2 332,221 + +0.9 332,221 + +0.8 332,221 + -0.1 332,222 - -0.3 332,222 - -0.8 332,222 - -0.3 332,222 - +0.8 332,313 + -0.8 332,314 - +1.1 332,314 - -0.2 332,320 - -0.5
Or see this region's nucleotide sequence