Experiment: TP2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt repA and repL overlap by 8 nucleotides repL and NOLOHH_24645 are separated by 232 nucleotides NOLOHH_24645 and NOLOHH_24650 are separated by 309 nucleotides
NOLOHH_24630: repA - incFII family plasmid replication initiator RepA, at 70,547 to 71,404
repA
NOLOHH_24635: repL - Positive regulator of RepFIC repA1 expression, at 71,397 to 71,471
repL
NOLOHH_24645: NOLOHH_24645 - replication protein RepA, at 71,704 to 71,961
_24645
NOLOHH_24650: NOLOHH_24650 - GNAT family N-acetyltransferase, at 72,271 to 72,753
_24650
Position (kb) 71
72 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 70.460 kb on + strand at 70.460 kb on + strand at 70.575 kb on + strand at 70.635 kb on - strand, within repA at 70.755 kb on - strand, within repA at 70.768 kb on + strand, within repA at 70.768 kb on + strand, within repA at 70.769 kb on - strand, within repA at 70.999 kb on - strand, within repA at 71.382 kb on + strand at 71.383 kb on - strand at 71.418 kb on + strand, within repL at 71.418 kb on + strand, within repL at 71.418 kb on + strand, within repL at 71.419 kb on - strand, within repL at 71.473 kb on + strand at 71.473 kb on + strand at 71.473 kb on + strand at 71.473 kb on + strand at 71.473 kb on + strand at 71.473 kb on + strand at 71.474 kb on - strand at 71.474 kb on - strand at 71.474 kb on - strand at 71.477 kb on + strand at 71.477 kb on + strand at 71.478 kb on - strand at 71.478 kb on - strand at 71.478 kb on - strand at 71.478 kb on - strand at 71.506 kb on + strand at 71.623 kb on + strand at 71.666 kb on + strand at 71.686 kb on - strand at 71.750 kb on - strand, within NOLOHH_24645 at 71.793 kb on + strand, within NOLOHH_24645 at 71.799 kb on - strand, within NOLOHH_24645 at 71.842 kb on + strand, within NOLOHH_24645 at 71.843 kb on - strand, within NOLOHH_24645 at 71.843 kb on - strand, within NOLOHH_24645 at 71.909 kb on + strand, within NOLOHH_24645 at 72.004 kb on + strand at 72.007 kb on + strand at 72.175 kb on + strand at 72.176 kb on - strand at 72.214 kb on + strand at 72.222 kb on - strand at 72.222 kb on - strand at 72.229 kb on - strand at 72.229 kb on - strand at 72.229 kb on - strand at 72.229 kb on - strand at 72.239 kb on - strand at 72.291 kb on + strand at 72.292 kb on - strand at 72.292 kb on - strand at 72.379 kb on - strand, within NOLOHH_24650 at 72.382 kb on - strand, within NOLOHH_24650 at 72.382 kb on - strand, within NOLOHH_24650 at 72.382 kb on - strand, within NOLOHH_24650 at 72.382 kb on - strand, within NOLOHH_24650 at 72.460 kb on - strand, within NOLOHH_24650
Per-strain Table
Position Strand Gene LocusTag Fraction TP2 remove 70,460 + +1.4 70,460 + -0.2 70,575 + -0.5 70,635 - repA NOLOHH_24630 0.10 +1.7 70,755 - repA NOLOHH_24630 0.24 +0.1 70,768 + repA NOLOHH_24630 0.26 -1.5 70,768 + repA NOLOHH_24630 0.26 +0.1 70,769 - repA NOLOHH_24630 0.26 +0.7 70,999 - repA NOLOHH_24630 0.53 +0.1 71,382 + -0.5 71,383 - -0.5 71,418 + repL NOLOHH_24635 0.28 +1.1 71,418 + repL NOLOHH_24635 0.28 -0.9 71,418 + repL NOLOHH_24635 0.28 -1.5 71,419 - repL NOLOHH_24635 0.29 -1.5 71,473 + -1.5 71,473 + +0.8 71,473 + +0.9 71,473 + +0.1 71,473 + -1.2 71,473 + +1.1 71,474 - -0.8 71,474 - -3.1 71,474 - +1.7 71,477 + -0.6 71,477 + -0.1 71,478 - +0.9 71,478 - +0.9 71,478 - +0.5 71,478 - -0.4 71,506 + -0.2 71,623 + -1.9 71,666 + +0.1 71,686 - -0.9 71,750 - NOLOHH_24645 0.18 +1.1 71,793 + NOLOHH_24645 0.34 -1.2 71,799 - NOLOHH_24645 0.37 +0.3 71,842 + NOLOHH_24645 0.53 -0.5 71,843 - NOLOHH_24645 0.54 +0.6 71,843 - NOLOHH_24645 0.54 +0.2 71,909 + NOLOHH_24645 0.79 +2.4 72,004 + +1.5 72,007 + -1.2 72,175 + -0.9 72,176 - -1.9 72,214 + +0.2 72,222 - -1.9 72,222 - -0.6 72,229 - +0.4 72,229 - -1.2 72,229 - +0.1 72,229 - +1.7 72,239 - -0.1 72,291 + -1.2 72,292 - +1.5 72,292 - +2.1 72,379 - NOLOHH_24650 0.22 -0.9 72,382 - NOLOHH_24650 0.23 +1.1 72,382 - NOLOHH_24650 0.23 -0.2 72,382 - NOLOHH_24650 0.23 +0.4 72,382 - NOLOHH_24650 0.23 +0.4 72,460 - NOLOHH_24650 0.39 +0.8
Or see this region's nucleotide sequence