Strain Fitness in Escherichia coli ECOR27 around NOLOHH_09940

Experiment: TP2

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nttruB and rpsO are separated by 148 nucleotidesrpsO and pnp are separated by 246 nucleotidespnp and nlpI are separated by 108 nucleotides NOLOHH_09925: truB - tRNA pseudouridine(55) synthase TruB, at 1,912,608 to 1,913,552 truB NOLOHH_09930: rpsO - 30S ribosomal protein S15, at 1,913,701 to 1,913,970 rpsO NOLOHH_09940: pnp - polyribonucleotide nucleotidyltransferase, at 1,914,217 to 1,916,352 pnp NOLOHH_09945: nlpI - lipoprotein NlpI, at 1,916,461 to 1,917,345 nlpI Position (kb) 1914 1915 1916 1917Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 1913.225 kb on + strand, within truBat 1913.226 kb on - strand, within truBat 1913.230 kb on - strand, within truBat 1913.298 kb on - strand, within truBat 1913.314 kb on + strand, within truBat 1913.394 kb on + strand, within truBat 1913.395 kb on - strand, within truBat 1913.421 kb on - strand, within truBat 1913.602 kb on + strandat 1913.621 kb on + strandat 1913.638 kb on + strandat 1914.044 kb on + strandat 1914.044 kb on + strandat 1914.049 kb on + strandat 1914.049 kb on + strandat 1914.049 kb on + strandat 1914.050 kb on - strandat 1914.050 kb on - strandat 1914.050 kb on - strandat 1914.050 kb on - strandat 1914.050 kb on - strandat 1914.050 kb on - strandat 1914.114 kb on + strandat 1914.114 kb on + strandat 1914.114 kb on + strandat 1914.114 kb on + strandat 1914.114 kb on + strandat 1914.114 kb on + strandat 1914.137 kb on + strandat 1914.166 kb on - strandat 1914.188 kb on - strandat 1914.213 kb on + strandat 1914.213 kb on + strandat 1914.214 kb on - strandat 1914.214 kb on - strandat 1914.222 kb on + strandat 1914.238 kb on - strandat 1914.309 kb on + strandat 1914.403 kb on + strandat 1914.403 kb on + strandat 1914.404 kb on - strandat 1914.414 kb on + strandat 1914.414 kb on + strandat 1914.414 kb on + strandat 1914.414 kb on + strandat 1914.418 kb on + strandat 1914.418 kb on + strandat 1914.419 kb on - strandat 1914.419 kb on - strandat 1914.677 kb on + strand, within pnpat 1914.682 kb on + strand, within pnpat 1914.682 kb on + strand, within pnpat 1914.683 kb on - strand, within pnpat 1914.697 kb on + strand, within pnpat 1914.698 kb on - strand, within pnpat 1914.702 kb on - strand, within pnpat 1915.134 kb on + strand, within pnpat 1915.333 kb on - strand, within pnpat 1915.355 kb on - strand, within pnpat 1915.355 kb on - strand, within pnpat 1915.490 kb on + strand, within pnpat 1915.490 kb on + strand, within pnpat 1915.490 kb on + strand, within pnpat 1915.491 kb on - strand, within pnpat 1915.491 kb on - strand, within pnpat 1915.491 kb on - strand, within pnpat 1915.496 kb on + strand, within pnpat 1915.496 kb on + strand, within pnpat 1915.496 kb on + strand, within pnpat 1915.496 kb on + strand, within pnpat 1915.497 kb on - strand, within pnpat 1915.497 kb on - strand, within pnpat 1915.533 kb on + strand, within pnpat 1915.533 kb on + strand, within pnpat 1915.534 kb on - strand, within pnpat 1915.534 kb on - strand, within pnpat 1915.639 kb on + strand, within pnpat 1915.639 kb on + strand, within pnpat 1915.639 kb on + strand, within pnpat 1915.639 kb on + strand, within pnpat 1915.639 kb on + strand, within pnpat 1915.639 kb on + strand, within pnpat 1915.639 kb on + strand, within pnpat 1915.639 kb on + strand, within pnpat 1915.640 kb on - strand, within pnpat 1915.640 kb on - strand, within pnpat 1915.640 kb on - strand, within pnpat 1915.640 kb on - strand, within pnpat 1915.640 kb on - strand, within pnpat 1915.640 kb on - strand, within pnpat 1915.640 kb on - strand, within pnpat 1915.646 kb on + strand, within pnpat 1915.812 kb on + strand, within pnpat 1915.884 kb on + strand, within pnpat 1915.972 kb on - strand, within pnpat 1915.972 kb on - strand, within pnpat 1915.972 kb on - strand, within pnpat 1916.040 kb on + strand, within pnpat 1916.040 kb on + strand, within pnpat 1916.089 kb on + strand, within pnpat 1916.097 kb on + strand, within pnpat 1916.109 kb on + strand, within pnpat 1916.210 kb on - strandat 1916.283 kb on + strandat 1916.390 kb on - strandat 1916.502 kb on + strandat 1916.502 kb on + strandat 1916.503 kb on - strandat 1916.503 kb on - strandat 1916.520 kb on + strandat 1916.520 kb on + strandat 1916.523 kb on + strandat 1916.523 kb on + strandat 1916.523 kb on + strandat 1916.523 kb on + strandat 1916.523 kb on + strandat 1916.535 kb on + strandat 1916.598 kb on + strand, within nlpIat 1916.598 kb on + strand, within nlpIat 1916.599 kb on - strand, within nlpIat 1916.599 kb on - strand, within nlpIat 1916.654 kb on + strand, within nlpIat 1916.654 kb on + strand, within nlpIat 1916.654 kb on + strand, within nlpIat 1916.655 kb on - strand, within nlpIat 1916.656 kb on + strand, within nlpIat 1916.674 kb on + strand, within nlpIat 1916.679 kb on + strand, within nlpIat 1916.765 kb on + strand, within nlpIat 1916.765 kb on + strand, within nlpIat 1916.771 kb on + strand, within nlpIat 1916.773 kb on + strand, within nlpIat 1916.773 kb on + strand, within nlpIat 1916.773 kb on + strand, within nlpIat 1916.773 kb on + strand, within nlpIat 1916.845 kb on + strand, within nlpIat 1916.845 kb on + strand, within nlpIat 1916.845 kb on + strand, within nlpIat 1916.845 kb on + strand, within nlpIat 1916.879 kb on + strand, within nlpIat 1916.881 kb on + strand, within nlpIat 1916.881 kb on + strand, within nlpIat 1916.971 kb on + strand, within nlpIat 1916.971 kb on + strand, within nlpIat 1917.076 kb on + strand, within nlpIat 1917.175 kb on + strand, within nlpIat 1917.175 kb on + strand, within nlpIat 1917.175 kb on + strand, within nlpIat 1917.175 kb on + strand, within nlpIat 1917.179 kb on + strand, within nlpIat 1917.179 kb on + strand, within nlpIat 1917.183 kb on + strand, within nlpIat 1917.187 kb on + strand, within nlpIat 1917.187 kb on + strand, within nlpIat 1917.187 kb on + strand, within nlpIat 1917.187 kb on + strand, within nlpIat 1917.187 kb on + strand, within nlpIat 1917.190 kb on + strand, within nlpIat 1917.258 kb on + strandat 1917.258 kb on + strandat 1917.343 kb on + strandat 1917.343 kb on + strandat 1917.352 kb on + strandat 1917.352 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction TP2
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1,913,225 + truB NOLOHH_09925 0.65 -1.3
1,913,226 - truB NOLOHH_09925 0.65 -0.3
1,913,230 - truB NOLOHH_09925 0.66 -1.3
1,913,298 - truB NOLOHH_09925 0.73 -0.5
1,913,314 + truB NOLOHH_09925 0.75 -0.9
1,913,394 + truB NOLOHH_09925 0.83 -1.1
1,913,395 - truB NOLOHH_09925 0.83 -0.9
1,913,421 - truB NOLOHH_09925 0.86 -1.8
1,913,602 + -1.5
1,913,621 + -2.5
1,913,638 + +1.1
1,914,044 + +1.1
1,914,044 + +0.2
1,914,049 + -0.0
1,914,049 + -0.9
1,914,049 + +3.4
1,914,050 - +0.7
1,914,050 - +1.3
1,914,050 - +2.1
1,914,050 - -2.3
1,914,050 - +1.1
1,914,050 - +0.3
1,914,114 + +1.3
1,914,114 + +0.1
1,914,114 + -1.3
1,914,114 + +0.5
1,914,114 + -0.5
1,914,114 + +0.9
1,914,137 + -1.9
1,914,166 - -1.8
1,914,188 - -1.5
1,914,213 + -0.8
1,914,213 + +1.8
1,914,214 - +1.1
1,914,214 - -0.5
1,914,222 + -0.5
1,914,238 - -0.5
1,914,309 + +0.6
1,914,403 + -1.9
1,914,403 + +0.1
1,914,404 - +3.1
1,914,414 + +0.2
1,914,414 + -2.9
1,914,414 + +0.5
1,914,414 + +0.8
1,914,418 + +0.1
1,914,418 + +0.1
1,914,419 - -0.3
1,914,419 - +2.1
1,914,677 + pnp NOLOHH_09940 0.22 -1.1
1,914,682 + pnp NOLOHH_09940 0.22 +0.2
1,914,682 + pnp NOLOHH_09940 0.22 -0.5
1,914,683 - pnp NOLOHH_09940 0.22 +1.1
1,914,697 + pnp NOLOHH_09940 0.22 +0.2
1,914,698 - pnp NOLOHH_09940 0.23 +1.1
1,914,702 - pnp NOLOHH_09940 0.23 +0.6
1,915,134 + pnp NOLOHH_09940 0.43 -1.5
1,915,333 - pnp NOLOHH_09940 0.52 -1.5
1,915,355 - pnp NOLOHH_09940 0.53 -1.3
1,915,355 - pnp NOLOHH_09940 0.53 -2.1
1,915,490 + pnp NOLOHH_09940 0.60 +1.1
1,915,490 + pnp NOLOHH_09940 0.60 -1.5
1,915,490 + pnp NOLOHH_09940 0.60 -1.9
1,915,491 - pnp NOLOHH_09940 0.60 -0.9
1,915,491 - pnp NOLOHH_09940 0.60 -0.4
1,915,491 - pnp NOLOHH_09940 0.60 -0.9
1,915,496 + pnp NOLOHH_09940 0.60 -1.3
1,915,496 + pnp NOLOHH_09940 0.60 +1.1
1,915,496 + pnp NOLOHH_09940 0.60 -0.9
1,915,496 + pnp NOLOHH_09940 0.60 +1.2
1,915,497 - pnp NOLOHH_09940 0.60 +0.6
1,915,497 - pnp NOLOHH_09940 0.60 -0.3
1,915,533 + pnp NOLOHH_09940 0.62 -0.5
1,915,533 + pnp NOLOHH_09940 0.62 +1.1
1,915,534 - pnp NOLOHH_09940 0.62 +1.1
1,915,534 - pnp NOLOHH_09940 0.62 +1.6
1,915,639 + pnp NOLOHH_09940 0.67 +0.9
1,915,639 + pnp NOLOHH_09940 0.67 +1.6
1,915,639 + pnp NOLOHH_09940 0.67 -1.1
1,915,639 + pnp NOLOHH_09940 0.67 +1.1
1,915,639 + pnp NOLOHH_09940 0.67 +1.5
1,915,639 + pnp NOLOHH_09940 0.67 -0.9
1,915,639 + pnp NOLOHH_09940 0.67 +3.1
1,915,639 + pnp NOLOHH_09940 0.67 -1.3
1,915,640 - pnp NOLOHH_09940 0.67 +1.1
1,915,640 - pnp NOLOHH_09940 0.67 +0.1
1,915,640 - pnp NOLOHH_09940 0.67 -2.1
1,915,640 - pnp NOLOHH_09940 0.67 -0.8
1,915,640 - pnp NOLOHH_09940 0.67 -1.9
1,915,640 - pnp NOLOHH_09940 0.67 +3.4
1,915,640 - pnp NOLOHH_09940 0.67 -1.5
1,915,646 + pnp NOLOHH_09940 0.67 +1.1
1,915,812 + pnp NOLOHH_09940 0.75 +1.1
1,915,884 + pnp NOLOHH_09940 0.78 +0.4
1,915,972 - pnp NOLOHH_09940 0.82 -0.9
1,915,972 - pnp NOLOHH_09940 0.82 +0.1
1,915,972 - pnp NOLOHH_09940 0.82 -2.3
1,916,040 + pnp NOLOHH_09940 0.85 -0.5
1,916,040 + pnp NOLOHH_09940 0.85 +0.2
1,916,089 + pnp NOLOHH_09940 0.88 -1.3
1,916,097 + pnp NOLOHH_09940 0.88 +0.5
1,916,109 + pnp NOLOHH_09940 0.89 +1.5
1,916,210 - +0.1
1,916,283 + -1.0
1,916,390 - +1.1
1,916,502 + -0.4
1,916,502 + +0.1
1,916,503 - +0.1
1,916,503 - +0.8
1,916,520 + -1.0
1,916,520 + -0.5
1,916,523 + +0.1
1,916,523 + +0.7
1,916,523 + +1.5
1,916,523 + -1.8
1,916,523 + +0.6
1,916,535 + -0.5
1,916,598 + nlpI NOLOHH_09945 0.15 +1.6
1,916,598 + nlpI NOLOHH_09945 0.15 +0.6
1,916,599 - nlpI NOLOHH_09945 0.16 +1.4
1,916,599 - nlpI NOLOHH_09945 0.16 +0.7
1,916,654 + nlpI NOLOHH_09945 0.22 +2.4
1,916,654 + nlpI NOLOHH_09945 0.22 -0.1
1,916,654 + nlpI NOLOHH_09945 0.22 +0.7
1,916,655 - nlpI NOLOHH_09945 0.22 +0.1
1,916,656 + nlpI NOLOHH_09945 0.22 -0.3
1,916,674 + nlpI NOLOHH_09945 0.24 +1.2
1,916,679 + nlpI NOLOHH_09945 0.25 +0.1
1,916,765 + nlpI NOLOHH_09945 0.34 +0.3
1,916,765 + nlpI NOLOHH_09945 0.34 -0.1
1,916,771 + nlpI NOLOHH_09945 0.35 +0.8
1,916,773 + nlpI NOLOHH_09945 0.35 -0.4
1,916,773 + nlpI NOLOHH_09945 0.35 +2.1
1,916,773 + nlpI NOLOHH_09945 0.35 +1.1
1,916,773 + nlpI NOLOHH_09945 0.35 +0.8
1,916,845 + nlpI NOLOHH_09945 0.43 -0.5
1,916,845 + nlpI NOLOHH_09945 0.43 +0.1
1,916,845 + nlpI NOLOHH_09945 0.43 +0.4
1,916,845 + nlpI NOLOHH_09945 0.43 +0.2
1,916,879 + nlpI NOLOHH_09945 0.47 -0.5
1,916,881 + nlpI NOLOHH_09945 0.47 +0.1
1,916,881 + nlpI NOLOHH_09945 0.47 -1.5
1,916,971 + nlpI NOLOHH_09945 0.58 +1.9
1,916,971 + nlpI NOLOHH_09945 0.58 -1.1
1,917,076 + nlpI NOLOHH_09945 0.69 +0.1
1,917,175 + nlpI NOLOHH_09945 0.81 -0.9
1,917,175 + nlpI NOLOHH_09945 0.81 -1.9
1,917,175 + nlpI NOLOHH_09945 0.81 -2.1
1,917,175 + nlpI NOLOHH_09945 0.81 -0.2
1,917,179 + nlpI NOLOHH_09945 0.81 +0.1
1,917,179 + nlpI NOLOHH_09945 0.81 +1.1
1,917,183 + nlpI NOLOHH_09945 0.82 +0.6
1,917,187 + nlpI NOLOHH_09945 0.82 -0.2
1,917,187 + nlpI NOLOHH_09945 0.82 -0.4
1,917,187 + nlpI NOLOHH_09945 0.82 +0.7
1,917,187 + nlpI NOLOHH_09945 0.82 +0.4
1,917,187 + nlpI NOLOHH_09945 0.82 +0.7
1,917,190 + nlpI NOLOHH_09945 0.82 -1.2
1,917,258 + +0.8
1,917,258 + -0.3
1,917,343 + -0.1
1,917,343 + -0.9
1,917,352 + +0.3
1,917,352 + -1.8

Or see this region's nucleotide sequence