Strain Fitness in Escherichia coli ECOR27 around NOLOHH_01955

Experiment: TP2

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntfdeC and NOLOHH_01955 are separated by 871 nucleotidesNOLOHH_01955 and ykgR are separated by 124 nucleotidesykgR and rpmE2-1 are separated by 361 nucleotidesrpmE2-1 and NOLOHH_01970 overlap by 1 nucleotides NOLOHH_01950: fdeC - inverse autotransporter adhesin FdeC, at 294,825 to 299,078 fdeC NOLOHH_01955: NOLOHH_01955 - hypothetical protein, at 299,950 to 300,069 _01955 NOLOHH_01960: ykgR - Uncharacterized membrane protein YkgR, at 300,194 to 300,295 ykgR NOLOHH_01965: rpmE2-1 - type B 50S ribosomal protein L31, at 300,657 to 300,920 rpmE2-1 NOLOHH_01970: NOLOHH_01970 - 50S ribosomal protein L36 2, at 300,920 to 301,060 _01970 Position (kb) 299 300 301Strain fitness (log2 ratio) -2 -1 0 1 2 3at 299.719 kb on - strandat 299.726 kb on + strandat 299.729 kb on + strandat 299.853 kb on + strandat 299.854 kb on - strandat 299.854 kb on - strandat 299.857 kb on + strandat 299.867 kb on - strandat 299.881 kb on + strandat 299.882 kb on - strandat 299.925 kb on - strandat 299.941 kb on + strandat 299.949 kb on + strandat 299.950 kb on - strandat 300.045 kb on + strand, within NOLOHH_01955at 300.045 kb on + strand, within NOLOHH_01955at 300.046 kb on - strand, within NOLOHH_01955at 300.046 kb on - strand, within NOLOHH_01955at 300.140 kb on - strandat 300.146 kb on - strandat 300.146 kb on - strandat 300.148 kb on - strandat 300.158 kb on - strandat 300.267 kb on - strand, within ykgRat 300.293 kb on + strandat 300.363 kb on + strandat 300.386 kb on - strandat 300.399 kb on + strandat 300.409 kb on + strandat 300.444 kb on - strandat 300.532 kb on + strandat 300.619 kb on + strandat 300.620 kb on - strandat 300.620 kb on - strandat 300.686 kb on + strand, within rpmE2-1at 300.687 kb on - strand, within rpmE2-1at 300.721 kb on - strand, within rpmE2-1at 300.721 kb on - strand, within rpmE2-1at 300.721 kb on - strand, within rpmE2-1at 300.725 kb on + strand, within rpmE2-1at 300.781 kb on - strand, within rpmE2-1at 300.781 kb on - strand, within rpmE2-1at 300.787 kb on - strand, within rpmE2-1at 300.825 kb on + strand, within rpmE2-1at 300.825 kb on + strand, within rpmE2-1at 300.826 kb on - strand, within rpmE2-1at 300.827 kb on + strand, within rpmE2-1at 300.827 kb on + strand, within rpmE2-1at 300.931 kb on + strandat 300.932 kb on - strandat 300.996 kb on + strand, within NOLOHH_01970at 300.996 kb on + strand, within NOLOHH_01970at 300.996 kb on + strand, within NOLOHH_01970at 300.996 kb on + strand, within NOLOHH_01970at 300.996 kb on + strand, within NOLOHH_01970at 300.998 kb on + strand, within NOLOHH_01970at 300.999 kb on - strand, within NOLOHH_01970at 300.999 kb on - strand, within NOLOHH_01970at 300.999 kb on - strand, within NOLOHH_01970at 300.999 kb on - strand, within NOLOHH_01970at 301.009 kb on + strand, within NOLOHH_01970at 301.027 kb on + strand, within NOLOHH_01970

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Per-strain Table

Position Strand Gene LocusTag Fraction TP2
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299,719 - +0.9
299,726 + +1.6
299,729 + -1.8
299,853 + +0.7
299,854 - +0.1
299,854 - +1.9
299,857 + -2.4
299,867 - +0.8
299,881 + -2.1
299,882 - -1.5
299,925 - +3.2
299,941 + +0.2
299,949 + +0.9
299,950 - -0.1
300,045 + NOLOHH_01955 0.79 -0.7
300,045 + NOLOHH_01955 0.79 +0.9
300,046 - NOLOHH_01955 0.80 +0.6
300,046 - NOLOHH_01955 0.80 -0.1
300,140 - +0.1
300,146 - +0.9
300,146 - +0.9
300,148 - -1.7
300,158 - -1.8
300,267 - ykgR NOLOHH_01960 0.72 +0.3
300,293 + -0.5
300,363 + -0.7
300,386 - -0.4
300,399 + +0.3
300,409 + +0.3
300,444 - -0.1
300,532 + +0.1
300,619 + -2.7
300,620 - +0.3
300,620 - -0.8
300,686 + rpmE2-1 NOLOHH_01965 0.11 +0.4
300,687 - rpmE2-1 NOLOHH_01965 0.11 -0.5
300,721 - rpmE2-1 NOLOHH_01965 0.24 +0.8
300,721 - rpmE2-1 NOLOHH_01965 0.24 -1.9
300,721 - rpmE2-1 NOLOHH_01965 0.24 -0.7
300,725 + rpmE2-1 NOLOHH_01965 0.26 -1.2
300,781 - rpmE2-1 NOLOHH_01965 0.47 +1.3
300,781 - rpmE2-1 NOLOHH_01965 0.47 +0.3
300,787 - rpmE2-1 NOLOHH_01965 0.49 -0.4
300,825 + rpmE2-1 NOLOHH_01965 0.64 +0.3
300,825 + rpmE2-1 NOLOHH_01965 0.64 +0.0
300,826 - rpmE2-1 NOLOHH_01965 0.64 -0.4
300,827 + rpmE2-1 NOLOHH_01965 0.64 -1.9
300,827 + rpmE2-1 NOLOHH_01965 0.64 +1.1
300,931 + -2.6
300,932 - -0.1
300,996 + NOLOHH_01970 0.54 +0.2
300,996 + NOLOHH_01970 0.54 +0.9
300,996 + NOLOHH_01970 0.54 -0.0
300,996 + NOLOHH_01970 0.54 +0.9
300,996 + NOLOHH_01970 0.54 -2.3
300,998 + NOLOHH_01970 0.55 +0.3
300,999 - NOLOHH_01970 0.56 -0.6
300,999 - NOLOHH_01970 0.56 +0.6
300,999 - NOLOHH_01970 0.56 -0.7
300,999 - NOLOHH_01970 0.56 +0.2
301,009 + NOLOHH_01970 0.63 +1.8
301,027 + NOLOHH_01970 0.76 -0.7

Or see this region's nucleotide sequence