Strain Fitness in Escherichia coli HS(pFamp)R (ATCC 700891) around OHPLBJKB_03631

Experiment: EBB2 MOI1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntOHPLBJKB_03629 and OHPLBJKB_03630 are separated by 87 nucleotidesOHPLBJKB_03630 and OHPLBJKB_03631 overlap by 1 nucleotidesOHPLBJKB_03631 and OHPLBJKB_03632 are separated by 42 nucleotides OHPLBJKB_03629: OHPLBJKB_03629 - FKBP-type 16 kDa peptidyl-prolyl cis-trans isomerase, at 3,812,928 to 3,813,377 _03629 OHPLBJKB_03630: OHPLBJKB_03630 - Lipoprotein signal peptidase, at 3,813,465 to 3,813,959 _03630 OHPLBJKB_03631: OHPLBJKB_03631 - Isoleucine--tRNA ligase, at 3,813,959 to 3,816,775 _03631 OHPLBJKB_03632: OHPLBJKB_03632 - Riboflavin biosynthesis protein RibF, at 3,816,818 to 3,817,759 _03632 Position (kb) 3813 3814 3815 3816 3817Strain fitness (log2 ratio) -2 -1 0 1at 3813.394 kb on - strandat 3813.443 kb on - strandat 3813.483 kb on - strandat 3813.483 kb on - strandat 3813.483 kb on - strandat 3813.483 kb on - strandat 3816.807 kb on - strandat 3816.820 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction EBB2 MOI1
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3,813,394 - -2.3
3,813,443 - -0.3
3,813,483 - +0.6
3,813,483 - +0.8
3,813,483 - -0.3
3,813,483 - +0.4
3,816,807 - +0.8
3,816,820 - -0.6

Or see this region's nucleotide sequence