Strain Fitness in Escherichia coli HS(pFamp)R (ATCC 700891) around OHPLBJKB_02174

Experiment: EBB2 MOI1

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	EBB2 MOI1
2,251,203	+				-1.8
2,251,247	+				+1.4
2,251,248	-				-0.5
2,251,341	+				-0.3
2,251,341	+				+1.5
2,251,341	+				+1.3
2,251,475	-		OHPLBJKB_02173	0.22	-0.5
2,251,672	+		OHPLBJKB_02173	0.48	-1.4
2,251,673	-		OHPLBJKB_02173	0.48	-0.1
2,251,675	+		OHPLBJKB_02173	0.48	+0.3
2,251,676	-		OHPLBJKB_02173	0.49	-1.0
2,251,676	-		OHPLBJKB_02173	0.49	+0.3
2,251,687	+		OHPLBJKB_02173	0.50	+1.4
2,251,688	-		OHPLBJKB_02173	0.50	-1.0
2,251,724	-		OHPLBJKB_02173	0.55	-1.3
2,251,724	-		OHPLBJKB_02173	0.55	+1.0
2,251,725	+		OHPLBJKB_02173	0.55	-0.4
2,251,725	+		OHPLBJKB_02173	0.55	+0.2
2,251,725	+		OHPLBJKB_02173	0.55	+0.1
2,251,725	+		OHPLBJKB_02173	0.55	+2.8
2,251,725	+		OHPLBJKB_02173	0.55	-0.7
2,251,725	+		OHPLBJKB_02173	0.55	+0.0
2,251,725	+		OHPLBJKB_02173	0.55	-0.5
2,251,726	-		OHPLBJKB_02173	0.55	+1.5
2,251,726	-		OHPLBJKB_02173	0.55	-1.4
2,251,727	+		OHPLBJKB_02173	0.55	+1.0
2,251,727	+		OHPLBJKB_02173	0.55	+0.3
2,251,727	+		OHPLBJKB_02173	0.55	+1.6
2,251,727	+		OHPLBJKB_02173	0.55	+0.5
2,251,728	-		OHPLBJKB_02173	0.56	-0.9
2,251,728	-		OHPLBJKB_02173	0.56	-1.0
2,251,728	-		OHPLBJKB_02173	0.56	+0.4
2,251,728	-		OHPLBJKB_02173	0.56	+1.1
2,251,728	-		OHPLBJKB_02173	0.56	+2.2
2,251,728	-		OHPLBJKB_02173	0.56	+0.7
2,251,728	-		OHPLBJKB_02173	0.56	-0.2
2,251,728	-		OHPLBJKB_02173	0.56	+1.0
2,251,728	-		OHPLBJKB_02173	0.56	-1.4
2,251,728	-		OHPLBJKB_02173	0.56	-0.8
2,251,738	+		OHPLBJKB_02173	0.57	+0.5
2,251,739	-		OHPLBJKB_02173	0.57	+0.0
2,251,832	-		OHPLBJKB_02173	0.69	+0.0
2,251,838	-		OHPLBJKB_02173	0.70	+3.3
2,251,914	+		OHPLBJKB_02173	0.80	+0.2
2,251,915	-		OHPLBJKB_02173	0.81	+1.0
2,251,915	-		OHPLBJKB_02173	0.81	-0.7
2,251,915	-		OHPLBJKB_02173	0.81	+0.3
2,251,921	+		OHPLBJKB_02173	0.81	-1.3
2,251,984	+		OHPLBJKB_02173	0.90	-1.3
2,252,011	+				+1.0
2,252,048	+				+0.5
2,252,091	+				+0.3
2,252,097	-				+0.2
2,252,111	-				+1.2
2,252,124	-				+0.0
2,252,156	-				+2.1
2,252,162	-				+1.6
2,252,162	-				-0.8
2,252,162	-				+0.6
2,252,366	+				+1.3
2,252,366	+				+1.6
2,252,366	+				+1.0
2,252,366	+				+0.5
2,252,366	+				-0.7
2,252,366	+				+0.5
2,252,367	-				+0.6
2,252,367	-				+0.1
2,252,367	-				-0.3
2,252,367	-				+1.2
2,252,367	-				-0.8
2,252,369	+				+1.6
2,252,398	+				-0.6
2,252,398	+				+0.4
2,252,398	+				-1.6
2,252,399	-				-1.2
2,252,399	-				-1.1
2,252,399	-				-1.3
2,252,444	+		OHPLBJKB_02174	0.12	-0.9
2,252,444	+		OHPLBJKB_02174	0.12	+0.2
2,252,458	+		OHPLBJKB_02174	0.13	+0.9
2,252,459	-		OHPLBJKB_02174	0.13	+0.2
2,252,510	+		OHPLBJKB_02174	0.15	-1.0
2,252,510	+		OHPLBJKB_02174	0.15	+0.2
2,252,510	+		OHPLBJKB_02174	0.15	+0.1
2,252,510	+		OHPLBJKB_02174	0.15	+1.0
2,252,511	-		OHPLBJKB_02174	0.15	-0.9
2,252,669	-		OHPLBJKB_02174	0.23	-0.7
2,252,736	-		OHPLBJKB_02174	0.26	-0.2
2,253,037	-		OHPLBJKB_02174	0.41	+0.9
2,253,090	-		OHPLBJKB_02174	0.44	-1.0
2,253,142	+		OHPLBJKB_02174	0.46	-0.4
2,253,151	+		OHPLBJKB_02174	0.47	-0.2
2,253,270	+		OHPLBJKB_02174	0.52	+0.7
2,253,280	+		OHPLBJKB_02174	0.53	-1.6
2,253,305	-		OHPLBJKB_02174	0.54	+0.3
2,253,333	-		OHPLBJKB_02174	0.56	+1.1
2,253,335	+		OHPLBJKB_02174	0.56	-0.3
2,253,525	+		OHPLBJKB_02174	0.65	+0.7
2,253,525	+		OHPLBJKB_02174	0.65	-0.8
2,253,525	+		OHPLBJKB_02174	0.65	-0.9
2,253,541	-		OHPLBJKB_02174	0.66	-0.2
2,253,589	+		OHPLBJKB_02174	0.68	+2.3
2,253,589	+		OHPLBJKB_02174	0.68	+0.7
2,253,590	-		OHPLBJKB_02174	0.68	+0.3
2,253,590	-		OHPLBJKB_02174	0.68	-0.9
2,253,592	+		OHPLBJKB_02174	0.68	-0.1
2,253,737	-		OHPLBJKB_02174	0.75	+0.1
2,253,950	+		OHPLBJKB_02174	0.86	-0.0
2,253,965	+		OHPLBJKB_02174	0.86	-0.4
2,254,030	-		OHPLBJKB_02174	0.90	+0.1
2,254,032	+		OHPLBJKB_02174	0.90	+2.3
2,254,032	+		OHPLBJKB_02174	0.90	+0.2
2,254,149	-				+1.0
2,254,237	+				-1.2
2,254,238	-				+0.1
2,254,239	+				-0.6
2,254,239	+				+1.7
2,254,239	+				+0.4
2,254,239	+				+1.0
2,254,239	+				+0.3
2,254,239	+				+0.6
2,254,239	+				+0.9
2,254,240	-				+1.0
2,254,240	-				-0.9
2,254,240	-				-0.2
2,254,240	-				+1.3
2,254,240	-				+0.8
2,254,240	-				+1.3
2,254,287	+				+1.1
2,254,289	+				-0.0
2,254,289	+				-0.8
2,254,295	+				-1.3
2,254,296	-				-0.8
2,254,332	-				-0.4
2,254,338	-		OHPLBJKB_02175	0.10	-0.8
2,254,341	-		OHPLBJKB_02175	0.11	-0.0
2,254,380	+		OHPLBJKB_02175	0.18	-0.0
2,254,380	+		OHPLBJKB_02175	0.18	+0.1
2,254,394	-		OHPLBJKB_02175	0.21	+0.2
2,254,397	-		OHPLBJKB_02175	0.21	+1.7
2,254,397	-		OHPLBJKB_02175	0.21	+0.7
2,254,429	+		OHPLBJKB_02175	0.28	+0.4
2,254,429	+		OHPLBJKB_02175	0.28	-0.5
2,254,474	+		OHPLBJKB_02175	0.36	+0.9
2,254,500	-		OHPLBJKB_02175	0.41	-0.0
2,254,571	+		OHPLBJKB_02175	0.55	-0.5
2,254,610	-		OHPLBJKB_02175	0.62	-0.5
2,254,610	-		OHPLBJKB_02175	0.62	+2.0
2,254,639	+		OHPLBJKB_02175	0.68	-1.1
2,254,640	-		OHPLBJKB_02175	0.68	+0.8
2,254,640	-		OHPLBJKB_02175	0.68	-1.1
2,254,701	-		OHPLBJKB_02175	0.80	-0.3
2,254,706	+		OHPLBJKB_02175	0.81	+0.5
2,254,707	-		OHPLBJKB_02175	0.81	+0.1
2,254,824	+				-1.0
2,254,825	-				+0.3
2,254,825	-				-0.6
2,254,826	+				+1.7
2,254,867	+				+1.0
2,255,189	+		OHPLBJKB_02176	0.28	-1.2