Strain Fitness in Escherichia coli HS(pFamp)R (ATCC 700891) around OHPLBJKB_02019

Experiment: EBB2 MOI1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntOHPLBJKB_02017 and OHPLBJKB_02018 are separated by 52 nucleotidesOHPLBJKB_02018 and OHPLBJKB_02019 are separated by 450 nucleotidesOHPLBJKB_02019 and OHPLBJKB_02020 are separated by 14 nucleotides OHPLBJKB_02017: OHPLBJKB_02017 - 50S ribosomal protein L35, at 2,093,825 to 2,094,022 _02017 OHPLBJKB_02018: OHPLBJKB_02018 - 50S ribosomal protein L20, at 2,094,075 to 2,094,431 _02018 OHPLBJKB_02019: OHPLBJKB_02019 - Phenylalanine--tRNA ligase alpha subunit, at 2,094,882 to 2,095,865 _02019 OHPLBJKB_02020: OHPLBJKB_02020 - Phenylalanine--tRNA ligase beta subunit, at 2,095,880 to 2,098,267 _02020 Position (kb) 2094 2095 2096Strain fitness (log2 ratio) -1 0 1at 2094.713 kb on + strandat 2094.766 kb on + strandat 2094.766 kb on + strandat 2094.770 kb on + strandat 2094.770 kb on + strandat 2094.812 kb on + strandat 2094.837 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction EBB2 MOI1
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2,094,713 + -0.1
2,094,766 + -0.1
2,094,766 + +0.6
2,094,770 + +0.9
2,094,770 + -0.5
2,094,812 + +1.0
2,094,837 + +0.5

Or see this region's nucleotide sequence