Experiment: MGL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MPMX19_01304 and MPMX19_01305 are separated by 21 nucleotides MPMX19_01305 and MPMX19_01306 are separated by 124 nucleotides MPMX19_01306 and MPMX19_01307 are separated by 45 nucleotides
MPMX19_01304: MPMX19_01304 - Phosphoribosylformylglycinamidine cyclo-ligase, at 1,361,363 to 1,362,424
_01304
MPMX19_01305: MPMX19_01305 - Phosphoribosylglycinamide formyltransferase, at 1,362,446 to 1,363,111
_01305
MPMX19_01306: MPMX19_01306 - hypothetical protein, at 1,363,236 to 1,363,379
_01306
MPMX19_01307: MPMX19_01307 - Valine--tRNA ligase, at 1,363,425 to 1,366,073
_01307
Position (kb)
1363
1364 Strain fitness (log2 ratio)
-2
-1
0
1 at 1362.422 kb on + strand at 1362.433 kb on + strand at 1362.433 kb on + strand at 1362.433 kb on + strand at 1362.433 kb on + strand at 1362.433 kb on + strand at 1362.434 kb on - strand at 1362.869 kb on + strand, within MPMX19_01305 at 1362.869 kb on + strand, within MPMX19_01305 at 1362.870 kb on - strand, within MPMX19_01305 at 1362.870 kb on - strand, within MPMX19_01305 at 1362.873 kb on + strand, within MPMX19_01305 at 1362.874 kb on - strand, within MPMX19_01305 at 1363.109 kb on + strand at 1363.110 kb on - strand at 1363.110 kb on - strand at 1363.110 kb on - strand at 1363.377 kb on + strand at 1363.377 kb on + strand at 1363.377 kb on + strand at 1363.378 kb on - strand at 1363.378 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MGL remove 1,362,422 + -1.2 1,362,433 + +0.1 1,362,433 + -2.0 1,362,433 + -2.6 1,362,433 + +0.4 1,362,433 + -0.3 1,362,434 - -1.3 1,362,869 + MPMX19_01305 0.64 -0.4 1,362,869 + MPMX19_01305 0.64 -0.7 1,362,870 - MPMX19_01305 0.64 -0.7 1,362,870 - MPMX19_01305 0.64 +0.4 1,362,873 + MPMX19_01305 0.64 -1.0 1,362,874 - MPMX19_01305 0.64 +0.6 1,363,109 + -1.2 1,363,110 - -0.6 1,363,110 - -1.4 1,363,110 - -1.4 1,363,377 + -0.7 1,363,377 + -0.9 1,363,377 + +0.4 1,363,378 - -1.0 1,363,378 - -1.0
Or see this region's nucleotide sequence