Strain Fitness in Escherichia coli ECOR27 around NOLOHH_20225

Experiment: Bas69

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntlapB and lapA are separated by 6 nucleotideslapA and pgpB are separated by 148 nucleotides NOLOHH_20220: lapB - lipopolysaccharide assembly protein LapB, at 3,982,113 to 3,983,282 lapB NOLOHH_20225: lapA - lipopolysaccharide assembly protein LapA, at 3,983,289 to 3,983,597 lapA NOLOHH_20230: pgpB - phosphatidylglycerophosphatase B, at 3,983,746 to 3,984,510 pgpB Position (kb) 3983 3984Strain fitness (log2 ratio) -2 -1 0 1 2 3at 3983.508 kb on - strand, within lapAat 3983.628 kb on - strandat 3983.665 kb on - strandat 3983.724 kb on - strandat 3983.866 kb on - strand, within pgpBat 3983.866 kb on - strand, within pgpBat 3983.866 kb on - strand, within pgpBat 3983.870 kb on - strand, within pgpBat 3983.946 kb on + strand, within pgpBat 3983.947 kb on - strand, within pgpBat 3984.075 kb on - strand, within pgpBat 3984.075 kb on - strand, within pgpBat 3984.075 kb on - strand, within pgpBat 3984.166 kb on - strand, within pgpBat 3984.166 kb on - strand, within pgpBat 3984.190 kb on - strand, within pgpBat 3984.281 kb on - strand, within pgpB

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas69
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3,983,508 - lapA NOLOHH_20225 0.71 -2.6
3,983,628 - +0.3
3,983,665 - +1.0
3,983,724 - +1.9
3,983,866 - pgpB NOLOHH_20230 0.16 +0.4
3,983,866 - pgpB NOLOHH_20230 0.16 -1.6
3,983,866 - pgpB NOLOHH_20230 0.16 +3.3
3,983,870 - pgpB NOLOHH_20230 0.16 -1.7
3,983,946 + pgpB NOLOHH_20230 0.26 +0.9
3,983,947 - pgpB NOLOHH_20230 0.26 +1.1
3,984,075 - pgpB NOLOHH_20230 0.43 -1.1
3,984,075 - pgpB NOLOHH_20230 0.43 -0.9
3,984,075 - pgpB NOLOHH_20230 0.43 -1.4
3,984,166 - pgpB NOLOHH_20230 0.55 +0.4
3,984,166 - pgpB NOLOHH_20230 0.55 -1.1
3,984,190 - pgpB NOLOHH_20230 0.58 +0.9
3,984,281 - pgpB NOLOHH_20230 0.70 +0.7

Or see this region's nucleotide sequence