Strain Fitness in Escherichia coli ECOR27 around NOLOHH_19990

Experiment: Bas69

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntycjY and ymjC are separated by 5 nucleotidesymjC and mpaA are separated by 100 nucleotidesmpaA and ycjG overlap by 26 nucleotidesycjG and tpx are separated by 118 nucleotidestpx and tyrR are separated by 43 nucleotides NOLOHH_19975: ycjY - Uncharacterized protein YcjY, at 3,932,906 to 3,932,971 ycjY NOLOHH_19980: ymjC - Protein YmjC, at 3,932,977 to 3,933,141 ymjC NOLOHH_19985: mpaA - murein tripeptide amidase MpaA, at 3,933,242 to 3,933,970 mpaA NOLOHH_19990: ycjG - L-Ala-D/L-Glu epimerase, at 3,933,945 to 3,934,910 ycjG NOLOHH_19995: tpx - thiol peroxidase, at 3,935,029 to 3,935,535 tpx NOLOHH_20000: tyrR - transcriptional regulator TyrR, at 3,935,579 to 3,937,120 tyrR Position (kb) 3933 3934 3935Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3 4 5 6 7at 3933.216 kb on + strandat 3933.219 kb on + strandat 3934.077 kb on - strand, within ycjGat 3934.179 kb on - strand, within ycjGat 3934.224 kb on - strand, within ycjGat 3934.440 kb on - strand, within ycjGat 3934.443 kb on - strand, within ycjGat 3934.443 kb on - strand, within ycjGat 3934.705 kb on - strand, within ycjGat 3934.725 kb on - strand, within ycjGat 3934.797 kb on - strand, within ycjGat 3934.873 kb on - strandat 3934.873 kb on - strandat 3934.873 kb on - strandat 3934.873 kb on - strandat 3934.873 kb on - strandat 3934.873 kb on - strandat 3934.873 kb on - strandat 3934.873 kb on - strandat 3934.894 kb on - strandat 3934.914 kb on + strandat 3935.222 kb on + strand, within tpxat 3935.222 kb on + strand, within tpxat 3935.538 kb on + strandat 3935.538 kb on + strandat 3935.538 kb on + strandat 3935.538 kb on + strandat 3935.541 kb on + strandat 3935.541 kb on + strandat 3935.624 kb on - strandat 3935.909 kb on - strand, within tyrR

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas69
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3,933,216 + +3.0
3,933,219 + +0.8
3,934,077 - ycjG NOLOHH_19990 0.14 -0.4
3,934,179 - ycjG NOLOHH_19990 0.24 +0.8
3,934,224 - ycjG NOLOHH_19990 0.29 -1.1
3,934,440 - ycjG NOLOHH_19990 0.51 +0.8
3,934,443 - ycjG NOLOHH_19990 0.52 +1.9
3,934,443 - ycjG NOLOHH_19990 0.52 +0.4
3,934,705 - ycjG NOLOHH_19990 0.79 -1.6
3,934,725 - ycjG NOLOHH_19990 0.81 -2.8
3,934,797 - ycjG NOLOHH_19990 0.88 +2.8
3,934,873 - +0.5
3,934,873 - +0.3
3,934,873 - -1.4
3,934,873 - -0.6
3,934,873 - -1.0
3,934,873 - +0.3
3,934,873 - +0.3
3,934,873 - +0.8
3,934,894 - +0.5
3,934,914 + +0.8
3,935,222 + tpx NOLOHH_19995 0.38 +0.8
3,935,222 + tpx NOLOHH_19995 0.38 -0.6
3,935,538 + -0.2
3,935,538 + +1.2
3,935,538 + +0.8
3,935,538 + +0.3
3,935,541 + +0.8
3,935,541 + -0.5
3,935,624 - +4.3
3,935,909 - tyrR NOLOHH_20000 0.21 +7.2

Or see this region's nucleotide sequence