Strain Fitness in Escherichia coli ECOR27 around NOLOHH_19435

Experiment: Bas69

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntpdxI and paaY are separated by 230 nucleotidespaaY and paaX overlap by 19 nucleotidespaaX and paaF are separated by 100 nucleotides NOLOHH_19425: pdxI - pyridoxine 4-dehydrogenase, at 3,830,960 to 3,831,820 pdxI NOLOHH_19430: paaY - phenylacetic acid degradation protein PaaY, at 3,832,051 to 3,832,641 paaY NOLOHH_19435: paaX - phenylacetic acid degradation operon negative regulatory protein PaaX, at 3,832,623 to 3,833,573 paaX NOLOHH_19440: paaF - phenylacetate--CoA ligase, at 3,833,674 to 3,834,987 paaF Position (kb) 3832 3833 3834Strain fitness (log2 ratio) -2 -1 0 1 2at 3831.809 kb on - strandat 3832.009 kb on - strandat 3832.405 kb on - strand, within paaYat 3832.405 kb on - strand, within paaYat 3832.625 kb on - strandat 3832.868 kb on - strand, within paaXat 3833.217 kb on - strand, within paaXat 3833.494 kb on + strandat 3833.495 kb on - strandat 3833.495 kb on - strandat 3833.926 kb on + strand, within paaFat 3833.977 kb on + strand, within paaFat 3834.037 kb on - strand, within paaFat 3834.428 kb on + strand, within paaFat 3834.451 kb on - strand, within paaFat 3834.451 kb on - strand, within paaF

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas69
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3,831,809 - -0.6
3,832,009 - +0.7
3,832,405 - paaY NOLOHH_19430 0.60 -1.3
3,832,405 - paaY NOLOHH_19430 0.60 -0.8
3,832,625 - -1.3
3,832,868 - paaX NOLOHH_19435 0.26 -2.4
3,833,217 - paaX NOLOHH_19435 0.62 +0.7
3,833,494 + -0.6
3,833,495 - -0.3
3,833,495 - +1.7
3,833,926 + paaF NOLOHH_19440 0.19 -0.3
3,833,977 + paaF NOLOHH_19440 0.23 +2.3
3,834,037 - paaF NOLOHH_19440 0.28 -2.3
3,834,428 + paaF NOLOHH_19440 0.57 +0.7
3,834,451 - paaF NOLOHH_19440 0.59 -0.5
3,834,451 - paaF NOLOHH_19440 0.59 -0.8

Or see this region's nucleotide sequence