Strain Fitness in Escherichia coli ECOR27 around NOLOHH_19420

Experiment: Bas69

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntynbB and pgsA overlap by 1 nucleotidespgsA and ydbD are separated by 170 nucleotidesydbD and pdxI are separated by 63 nucleotides NOLOHH_19410: ynbB - Uncharacterized protein YnbB, at 3,826,918 to 3,827,814 ynbB NOLOHH_19415: pgsA - CDP-alcohol phosphatidyltransferase, at 3,827,814 to 3,828,419 pgsA NOLOHH_19420: ydbD - Uncharacterized protein YdbD, at 3,828,590 to 3,830,896 ydbD NOLOHH_19425: pdxI - pyridoxine 4-dehydrogenase, at 3,830,960 to 3,831,820 pdxI Position (kb) 3828 3829 3830 3831Strain fitness (log2 ratio) -2 -1 0 1 2 3at 3828.735 kb on - strandat 3828.949 kb on - strand, within ydbDat 3829.857 kb on - strand, within ydbDat 3830.018 kb on - strand, within ydbDat 3831.346 kb on + strand, within pdxIat 3831.809 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas69
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3,828,735 - +3.3
3,828,949 - ydbD NOLOHH_19420 0.16 -2.3
3,829,857 - ydbD NOLOHH_19420 0.55 +0.7
3,830,018 - ydbD NOLOHH_19420 0.62 -0.8
3,831,346 + pdxI NOLOHH_19425 0.45 -0.3
3,831,809 - -0.6

Or see this region's nucleotide sequence