Strain Fitness in Escherichia coli ECOR27 around NOLOHH_18120

Experiment: Bas69

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nthdhA and uidR are separated by 240 nucleotidesuidR and uidA are separated by 388 nucleotides NOLOHH_18115: hdhA - 7-alpha-hydroxysteroid dehydrogenase, at 3,555,177 to 3,555,944 hdhA NOLOHH_18120: uidR - DNA-binding transcriptional regulator UidR, at 3,556,185 to 3,556,763 uidR NOLOHH_18125: uidA - beta-glucuronidase, at 3,557,152 to 3,558,963 uidA Position (kb) 3556 3557Strain fitness (log2 ratio) -2 -1 0 1 2at 3555.242 kb on + strandat 3555.331 kb on + strand, within hdhAat 3555.662 kb on + strand, within hdhAat 3555.845 kb on + strand, within hdhAat 3556.161 kb on + strandat 3556.161 kb on + strandat 3556.162 kb on - strandat 3556.428 kb on + strand, within uidRat 3556.645 kb on + strand, within uidRat 3556.662 kb on + strand, within uidRat 3556.884 kb on - strandat 3557.073 kb on + strandat 3557.156 kb on + strandat 3557.334 kb on + strand, within uidAat 3557.512 kb on + strand, within uidAat 3557.534 kb on + strand, within uidAat 3557.534 kb on + strand, within uidAat 3557.538 kb on + strand, within uidAat 3557.538 kb on + strand, within uidAat 3557.629 kb on + strand, within uidA

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas69
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3,555,242 + -0.9
3,555,331 + hdhA NOLOHH_18115 0.20 -1.9
3,555,662 + hdhA NOLOHH_18115 0.63 +0.7
3,555,845 + hdhA NOLOHH_18115 0.87 -0.3
3,556,161 + +0.7
3,556,161 + +1.3
3,556,162 - +0.7
3,556,428 + uidR NOLOHH_18120 0.42 -0.9
3,556,645 + uidR NOLOHH_18120 0.79 -0.9
3,556,662 + uidR NOLOHH_18120 0.82 -0.3
3,556,884 - -0.3
3,557,073 + +0.7
3,557,156 + -0.0
3,557,334 + uidA NOLOHH_18125 0.10 +2.3
3,557,512 + uidA NOLOHH_18125 0.20 +1.3
3,557,534 + uidA NOLOHH_18125 0.21 -0.8
3,557,534 + uidA NOLOHH_18125 0.21 -0.8
3,557,538 + uidA NOLOHH_18125 0.21 +0.7
3,557,538 + uidA NOLOHH_18125 0.21 -0.8
3,557,629 + uidA NOLOHH_18125 0.26 +1.7

Or see this region's nucleotide sequence