Experiment: Bas69
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt fliH and fliG overlap by 8 nucleotides fliG and fliF overlap by 8 nucleotides fliF and fliE are separated by 214 nucleotides fliE and yedK are separated by 392 nucleotides
NOLOHH_16335: fliH - flagellar assembly protein FliH, at 3,216,759 to 3,217,445
fliH
NOLOHH_16340: fliG - flagellar motor switch protein FliG, at 3,217,438 to 3,218,433
fliG
NOLOHH_16345: fliF - flagellar M-ring protein FliF, at 3,218,426 to 3,220,084
fliF
NOLOHH_16350: fliE - flagellar hook-basal body complex protein FliE, at 3,220,299 to 3,220,613
fliE
NOLOHH_16355: yedK - Abasic site processing protein YedK, at 3,221,006 to 3,221,674
yedK
Position (kb)
3218
3219
3220
3221 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3217.445 kb on + strand at 3217.446 kb on - strand at 3217.446 kb on - strand at 3217.446 kb on - strand at 3217.446 kb on - strand at 3217.468 kb on + strand at 3217.469 kb on - strand at 3217.469 kb on - strand at 3217.900 kb on + strand, within fliG at 3218.146 kb on - strand, within fliG at 3218.278 kb on + strand, within fliG at 3218.279 kb on - strand, within fliG at 3218.445 kb on - strand at 3218.503 kb on + strand at 3218.628 kb on - strand, within fliF at 3218.628 kb on - strand, within fliF at 3218.795 kb on - strand, within fliF at 3218.987 kb on - strand, within fliF at 3219.012 kb on + strand, within fliF at 3219.310 kb on + strand, within fliF at 3219.384 kb on + strand, within fliF at 3219.385 kb on - strand, within fliF at 3219.403 kb on + strand, within fliF at 3219.421 kb on + strand, within fliF at 3219.509 kb on + strand, within fliF at 3219.509 kb on + strand, within fliF at 3219.510 kb on - strand, within fliF at 3219.510 kb on - strand, within fliF at 3219.510 kb on - strand, within fliF at 3219.510 kb on - strand, within fliF at 3219.560 kb on + strand, within fliF at 3219.749 kb on + strand, within fliF at 3219.943 kb on - strand at 3220.180 kb on - strand at 3220.231 kb on + strand at 3220.231 kb on + strand at 3220.231 kb on + strand at 3220.395 kb on - strand, within fliE at 3220.631 kb on + strand at 3220.780 kb on - strand at 3220.893 kb on + strand at 3220.910 kb on + strand at 3220.957 kb on + strand at 3221.021 kb on + strand at 3221.047 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bas69 remove 3,217,445 + -1.2 3,217,446 - +0.2 3,217,446 - -1.5 3,217,446 - -2.8 3,217,446 - -0.2 3,217,468 + -2.6 3,217,469 - +0.8 3,217,469 - -0.2 3,217,900 + fliG NOLOHH_16340 0.46 -0.2 3,218,146 - fliG NOLOHH_16340 0.71 -1.8 3,218,278 + fliG NOLOHH_16340 0.84 -0.2 3,218,279 - fliG NOLOHH_16340 0.84 -2.0 3,218,445 - +0.8 3,218,503 + -0.2 3,218,628 - fliF NOLOHH_16345 0.12 -1.8 3,218,628 - fliF NOLOHH_16345 0.12 -0.2 3,218,795 - fliF NOLOHH_16345 0.22 -1.2 3,218,987 - fliF NOLOHH_16345 0.34 -1.8 3,219,012 + fliF NOLOHH_16345 0.35 +0.8 3,219,310 + fliF NOLOHH_16345 0.53 -1.8 3,219,384 + fliF NOLOHH_16345 0.58 -2.0 3,219,385 - fliF NOLOHH_16345 0.58 -0.2 3,219,403 + fliF NOLOHH_16345 0.59 -2.4 3,219,421 + fliF NOLOHH_16345 0.60 -1.5 3,219,509 + fliF NOLOHH_16345 0.65 -2.0 3,219,509 + fliF NOLOHH_16345 0.65 +0.8 3,219,510 - fliF NOLOHH_16345 0.65 -0.2 3,219,510 - fliF NOLOHH_16345 0.65 -2.4 3,219,510 - fliF NOLOHH_16345 0.65 -1.2 3,219,510 - fliF NOLOHH_16345 0.65 -2.2 3,219,560 + fliF NOLOHH_16345 0.68 -2.0 3,219,749 + fliF NOLOHH_16345 0.80 -0.2 3,219,943 - -1.5 3,220,180 - +0.8 3,220,231 + -0.8 3,220,231 + -1.2 3,220,231 + -3.5 3,220,395 - fliE NOLOHH_16350 0.30 +0.8 3,220,631 + -0.2 3,220,780 - -0.2 3,220,893 + +1.8 3,220,910 + -0.5 3,220,957 + -0.2 3,221,021 + +0.8 3,221,047 + +0.8
Or see this region's nucleotide sequence