Strain Fitness in Escherichia coli ECOR27 around NOLOHH_16320

Experiment: Bas69

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntfliM and fliL are separated by 4 nucleotidesfliL and fliK are separated by 104 nucleotidesfliK and fliJ overlap by 4 nucleotidesfliJ and fliI are separated by 18 nucleotides NOLOHH_16310: fliM - flagellar motor switch protein FliM, at 3,212,222 to 3,213,226 fliM NOLOHH_16315: fliL - flagellar basal body-associated protein FliL, at 3,213,231 to 3,213,695 fliL NOLOHH_16320: fliK - flagellar hook length control protein FliK, at 3,213,800 to 3,214,927 fliK NOLOHH_16325: fliJ - flagella biosynthesis chaperone FliJ, at 3,214,924 to 3,215,367 fliJ NOLOHH_16330: fliI - flagellum-specific ATP synthase FliI, at 3,215,386 to 3,216,759 fliI Position (kb) 3213 3214 3215Strain fitness (log2 ratio) -2 -1 0 1at 3213.215 kb on - strandat 3213.475 kb on + strand, within fliLat 3213.596 kb on - strand, within fliLat 3213.596 kb on - strand, within fliLat 3213.679 kb on - strandat 3213.737 kb on + strandat 3213.834 kb on - strandat 3213.845 kb on + strandat 3213.868 kb on + strandat 3213.902 kb on + strandat 3214.049 kb on + strand, within fliKat 3214.049 kb on + strand, within fliKat 3214.050 kb on - strand, within fliKat 3214.186 kb on + strand, within fliKat 3214.282 kb on + strand, within fliKat 3214.339 kb on + strand, within fliKat 3214.367 kb on + strand, within fliKat 3215.107 kb on - strand, within fliJat 3215.163 kb on - strand, within fliJat 3215.532 kb on - strand, within fliIat 3215.781 kb on + strand, within fliIat 3215.783 kb on + strand, within fliIat 3215.783 kb on + strand, within fliI

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas69
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3,213,215 - -1.2
3,213,475 + fliL NOLOHH_16315 0.52 +0.8
3,213,596 - fliL NOLOHH_16315 0.78 -1.8
3,213,596 - fliL NOLOHH_16315 0.78 -1.2
3,213,679 - -0.2
3,213,737 + -0.2
3,213,834 - -2.0
3,213,845 + -1.5
3,213,868 + -1.2
3,213,902 + +0.4
3,214,049 + fliK NOLOHH_16320 0.22 -1.0
3,214,049 + fliK NOLOHH_16320 0.22 -1.2
3,214,050 - fliK NOLOHH_16320 0.22 -1.5
3,214,186 + fliK NOLOHH_16320 0.34 -0.2
3,214,282 + fliK NOLOHH_16320 0.43 -1.5
3,214,339 + fliK NOLOHH_16320 0.48 +0.8
3,214,367 + fliK NOLOHH_16320 0.50 -1.8
3,215,107 - fliJ NOLOHH_16325 0.41 -1.8
3,215,163 - fliJ NOLOHH_16325 0.54 -0.4
3,215,532 - fliI NOLOHH_16330 0.11 -2.5
3,215,781 + fliI NOLOHH_16330 0.29 -0.2
3,215,783 + fliI NOLOHH_16330 0.29 -0.2
3,215,783 + fliI NOLOHH_16330 0.29 -0.2

Or see this region's nucleotide sequence