Strain Fitness in Escherichia coli ECOR27 around NOLOHH_15960

Experiment: Bas69

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntpduO and ccmL are separated by 11 nucleotidesccmL and pduM are separated by 3 nucleotidespduM and NOLOHH_15965 overlap by 4 nucleotidesNOLOHH_15965 and NOLOHH_15970 are separated by 2 nucleotides NOLOHH_15950: pduO - two-domain cob(I)yrinic acid a,c-diamide adenosyltransferase PduO, at 3,139,460 to 3,140,467 pduO NOLOHH_15955: ccmL - ethanolamine utilization protein EutN, at 3,140,479 to 3,140,754 ccmL NOLOHH_15960: pduM - microcompartment protein PduM, at 3,140,758 to 3,141,249 pduM NOLOHH_15965: NOLOHH_15965 - propanediol utilization protein, at 3,141,246 to 3,141,878 _15965 NOLOHH_15970: NOLOHH_15970 - microcompartment protein, at 3,141,881 to 3,142,303 _15970 Position (kb) 3140 3141 3142Strain fitness (log2 ratio) -2 -1 0 1 2at 3139.759 kb on + strand, within pduOat 3139.808 kb on - strand, within pduOat 3139.898 kb on + strand, within pduOat 3139.899 kb on - strand, within pduOat 3140.011 kb on + strand, within pduOat 3140.011 kb on + strand, within pduOat 3140.014 kb on - strand, within pduOat 3140.218 kb on - strand, within pduOat 3140.335 kb on - strand, within pduOat 3140.390 kb on + strandat 3140.390 kb on + strandat 3140.755 kb on - strandat 3140.755 kb on - strandat 3140.857 kb on + strand, within pduMat 3140.903 kb on + strand, within pduMat 3140.903 kb on + strand, within pduMat 3140.936 kb on - strand, within pduMat 3141.109 kb on + strand, within pduMat 3141.120 kb on - strand, within pduMat 3141.321 kb on - strand, within NOLOHH_15965at 3141.434 kb on - strand, within NOLOHH_15965at 3141.434 kb on - strand, within NOLOHH_15965at 3141.458 kb on + strand, within NOLOHH_15965at 3141.458 kb on + strand, within NOLOHH_15965at 3141.459 kb on - strand, within NOLOHH_15965at 3141.459 kb on - strand, within NOLOHH_15965at 3141.459 kb on - strand, within NOLOHH_15965at 3141.459 kb on - strand, within NOLOHH_15965at 3141.459 kb on - strand, within NOLOHH_15965at 3141.459 kb on - strand, within NOLOHH_15965at 3141.459 kb on - strand, within NOLOHH_15965at 3141.459 kb on - strand, within NOLOHH_15965at 3141.519 kb on + strand, within NOLOHH_15965at 3141.704 kb on - strand, within NOLOHH_15965at 3141.704 kb on - strand, within NOLOHH_15965at 3141.767 kb on - strand, within NOLOHH_15965at 3141.787 kb on - strand, within NOLOHH_15965at 3141.787 kb on - strand, within NOLOHH_15965at 3141.787 kb on - strand, within NOLOHH_15965at 3141.788 kb on + strand, within NOLOHH_15965at 3141.964 kb on + strand, within NOLOHH_15970at 3141.964 kb on + strand, within NOLOHH_15970

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas69
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3,139,759 + pduO NOLOHH_15950 0.30 +1.7
3,139,808 - pduO NOLOHH_15950 0.35 -0.2
3,139,898 + pduO NOLOHH_15950 0.43 +0.8
3,139,899 - pduO NOLOHH_15950 0.44 -0.2
3,140,011 + pduO NOLOHH_15950 0.55 -1.7
3,140,011 + pduO NOLOHH_15950 0.55 +0.8
3,140,014 - pduO NOLOHH_15950 0.55 -0.2
3,140,218 - pduO NOLOHH_15950 0.75 -0.2
3,140,335 - pduO NOLOHH_15950 0.87 -2.0
3,140,390 + +0.3
3,140,390 + -1.5
3,140,755 - +2.2
3,140,755 - -1.5
3,140,857 + pduM NOLOHH_15960 0.20 -0.7
3,140,903 + pduM NOLOHH_15960 0.29 -1.2
3,140,903 + pduM NOLOHH_15960 0.29 -1.5
3,140,936 - pduM NOLOHH_15960 0.36 -2.3
3,141,109 + pduM NOLOHH_15960 0.71 -1.2
3,141,120 - pduM NOLOHH_15960 0.74 -1.7
3,141,321 - NOLOHH_15965 0.12 +0.8
3,141,434 - NOLOHH_15965 0.30 +0.6
3,141,434 - NOLOHH_15965 0.30 +2.1
3,141,458 + NOLOHH_15965 0.33 +0.8
3,141,458 + NOLOHH_15965 0.33 +0.8
3,141,459 - NOLOHH_15965 0.34 -0.7
3,141,459 - NOLOHH_15965 0.34 -0.9
3,141,459 - NOLOHH_15965 0.34 -1.7
3,141,459 - NOLOHH_15965 0.34 -0.2
3,141,459 - NOLOHH_15965 0.34 -1.5
3,141,459 - NOLOHH_15965 0.34 -1.5
3,141,459 - NOLOHH_15965 0.34 +0.4
3,141,459 - NOLOHH_15965 0.34 +1.2
3,141,519 + NOLOHH_15965 0.43 +0.8
3,141,704 - NOLOHH_15965 0.72 -0.2
3,141,704 - NOLOHH_15965 0.72 -0.7
3,141,767 - NOLOHH_15965 0.82 -0.2
3,141,787 - NOLOHH_15965 0.85 +1.1
3,141,787 - NOLOHH_15965 0.85 +0.3
3,141,787 - NOLOHH_15965 0.85 +1.8
3,141,788 + NOLOHH_15965 0.86 -0.2
3,141,964 + NOLOHH_15970 0.20 +2.1
3,141,964 + NOLOHH_15970 0.20 +0.2

Or see this region's nucleotide sequence