Strain Fitness in Escherichia coli ECOR27 around NOLOHH_12470

Experiment: Bas69

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntascF and ascG are separated by 256 nucleotidesascG and hydN are separated by 148 nucleotideshydN and hypF are separated by 152 nucleotides NOLOHH_12465: ascF - PTS cellobiose/arbutin/salicin transporter subunit IIBC, at 2,421,635 to 2,423,092 ascF NOLOHH_12470: ascG - DNA-binding transcriptional regulator AscG, at 2,423,349 to 2,424,362 ascG NOLOHH_12475: hydN - electron transport protein HydN, at 2,424,511 to 2,425,038 hydN NOLOHH_12480: hypF - carbamoyltransferase HypF, at 2,425,191 to 2,427,443 hypF Position (kb) 2423 2424 2425Strain fitness (log2 ratio) -2 -1 0 1 2 3at 2422.369 kb on + strand, within ascFat 2422.369 kb on + strand, within ascFat 2422.370 kb on - strand, within ascFat 2422.393 kb on + strand, within ascFat 2422.395 kb on + strand, within ascFat 2422.395 kb on + strand, within ascFat 2422.395 kb on + strand, within ascFat 2422.395 kb on + strand, within ascFat 2422.395 kb on + strand, within ascFat 2422.395 kb on + strand, within ascFat 2422.396 kb on - strand, within ascFat 2422.396 kb on - strand, within ascFat 2422.437 kb on + strand, within ascFat 2422.438 kb on - strand, within ascFat 2422.441 kb on + strand, within ascFat 2422.442 kb on - strand, within ascFat 2422.442 kb on - strand, within ascFat 2422.442 kb on - strand, within ascFat 2422.443 kb on + strand, within ascFat 2422.443 kb on + strand, within ascFat 2422.444 kb on - strand, within ascFat 2422.444 kb on - strand, within ascFat 2422.444 kb on - strand, within ascFat 2422.529 kb on + strand, within ascFat 2422.565 kb on - strand, within ascFat 2422.565 kb on - strand, within ascFat 2422.838 kb on - strand, within ascFat 2422.838 kb on - strand, within ascFat 2422.838 kb on - strand, within ascFat 2422.838 kb on - strand, within ascFat 2422.886 kb on - strand, within ascFat 2422.886 kb on - strand, within ascFat 2422.918 kb on + strand, within ascFat 2422.919 kb on - strand, within ascFat 2422.923 kb on - strand, within ascFat 2422.923 kb on - strand, within ascFat 2423.008 kb on + strandat 2423.008 kb on + strandat 2423.009 kb on - strandat 2423.009 kb on - strandat 2423.009 kb on - strandat 2423.114 kb on + strandat 2423.119 kb on + strandat 2423.119 kb on + strandat 2423.119 kb on + strandat 2423.120 kb on - strandat 2423.120 kb on - strandat 2423.125 kb on + strandat 2423.125 kb on + strandat 2423.125 kb on + strandat 2423.126 kb on - strandat 2423.126 kb on - strandat 2423.272 kb on - strandat 2423.316 kb on + strandat 2423.316 kb on + strandat 2423.337 kb on - strandat 2423.430 kb on + strandat 2423.430 kb on + strandat 2423.526 kb on - strand, within ascGat 2423.545 kb on + strand, within ascGat 2423.562 kb on + strand, within ascGat 2423.574 kb on + strand, within ascGat 2423.587 kb on + strand, within ascGat 2423.685 kb on + strand, within ascGat 2423.686 kb on - strand, within ascGat 2423.714 kb on + strand, within ascGat 2423.823 kb on - strand, within ascGat 2423.823 kb on - strand, within ascGat 2423.840 kb on + strand, within ascGat 2423.855 kb on + strand, within ascGat 2423.856 kb on - strand, within ascGat 2423.954 kb on + strand, within ascGat 2423.955 kb on - strand, within ascGat 2423.955 kb on - strand, within ascGat 2423.955 kb on - strand, within ascGat 2423.978 kb on + strand, within ascGat 2424.082 kb on + strand, within ascGat 2424.082 kb on + strand, within ascGat 2424.540 kb on + strandat 2424.547 kb on - strandat 2424.833 kb on + strand, within hydNat 2424.833 kb on + strand, within hydNat 2424.898 kb on - strand, within hydNat 2424.898 kb on - strand, within hydNat 2424.965 kb on + strand, within hydNat 2424.966 kb on - strand, within hydNat 2424.981 kb on + strand, within hydNat 2424.982 kb on - strand, within hydNat 2425.083 kb on - strandat 2425.088 kb on + strandat 2425.089 kb on - strandat 2425.227 kb on - strandat 2425.288 kb on + strandat 2425.289 kb on - strandat 2425.289 kb on - strandat 2425.289 kb on - strandat 2425.289 kb on - strandat 2425.289 kb on - strandat 2425.310 kb on + strandat 2425.311 kb on - strandat 2425.327 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas69
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2,422,369 + ascF NOLOHH_12465 0.50 +0.9
2,422,369 + ascF NOLOHH_12465 0.50 -0.5
2,422,370 - ascF NOLOHH_12465 0.50 -1.1
2,422,393 + ascF NOLOHH_12465 0.52 -0.5
2,422,395 + ascF NOLOHH_12465 0.52 +3.2
2,422,395 + ascF NOLOHH_12465 0.52 -0.4
2,422,395 + ascF NOLOHH_12465 0.52 +1.9
2,422,395 + ascF NOLOHH_12465 0.52 -2.3
2,422,395 + ascF NOLOHH_12465 0.52 -1.5
2,422,395 + ascF NOLOHH_12465 0.52 +0.9
2,422,396 - ascF NOLOHH_12465 0.52 -0.1
2,422,396 - ascF NOLOHH_12465 0.52 +1.2
2,422,437 + ascF NOLOHH_12465 0.55 +1.3
2,422,438 - ascF NOLOHH_12465 0.55 -0.8
2,422,441 + ascF NOLOHH_12465 0.55 -0.7
2,422,442 - ascF NOLOHH_12465 0.55 -0.1
2,422,442 - ascF NOLOHH_12465 0.55 +0.1
2,422,442 - ascF NOLOHH_12465 0.55 -1.7
2,422,443 + ascF NOLOHH_12465 0.55 -2.3
2,422,443 + ascF NOLOHH_12465 0.55 +0.0
2,422,444 - ascF NOLOHH_12465 0.55 +0.0
2,422,444 - ascF NOLOHH_12465 0.55 -0.7
2,422,444 - ascF NOLOHH_12465 0.55 +0.3
2,422,529 + ascF NOLOHH_12465 0.61 -0.1
2,422,565 - ascF NOLOHH_12465 0.64 +0.6
2,422,565 - ascF NOLOHH_12465 0.64 +0.9
2,422,838 - ascF NOLOHH_12465 0.83 -0.2
2,422,838 - ascF NOLOHH_12465 0.83 -0.4
2,422,838 - ascF NOLOHH_12465 0.83 +1.3
2,422,838 - ascF NOLOHH_12465 0.83 +0.3
2,422,886 - ascF NOLOHH_12465 0.86 -1.1
2,422,886 - ascF NOLOHH_12465 0.86 -0.1
2,422,918 + ascF NOLOHH_12465 0.88 +0.2
2,422,919 - ascF NOLOHH_12465 0.88 +0.7
2,422,923 - ascF NOLOHH_12465 0.88 +0.9
2,422,923 - ascF NOLOHH_12465 0.88 -0.5
2,423,008 + +0.4
2,423,008 + +0.3
2,423,009 - -0.7
2,423,009 - -0.1
2,423,009 - +0.3
2,423,114 + -0.6
2,423,119 + -0.2
2,423,119 + +0.8
2,423,119 + -0.4
2,423,120 - -0.5
2,423,120 - -0.7
2,423,125 + +1.3
2,423,125 + -0.7
2,423,125 + -2.7
2,423,126 - -2.0
2,423,126 - +0.3
2,423,272 - -0.4
2,423,316 + +0.5
2,423,316 + +0.0
2,423,337 - -0.2
2,423,430 + -0.2
2,423,430 + +1.0
2,423,526 - ascG NOLOHH_12470 0.17 +0.8
2,423,545 + ascG NOLOHH_12470 0.19 -0.0
2,423,562 + ascG NOLOHH_12470 0.21 +1.2
2,423,574 + ascG NOLOHH_12470 0.22 +0.0
2,423,587 + ascG NOLOHH_12470 0.23 -1.5
2,423,685 + ascG NOLOHH_12470 0.33 -0.6
2,423,686 - ascG NOLOHH_12470 0.33 -1.2
2,423,714 + ascG NOLOHH_12470 0.36 -2.6
2,423,823 - ascG NOLOHH_12470 0.47 -0.6
2,423,823 - ascG NOLOHH_12470 0.47 -2.3
2,423,840 + ascG NOLOHH_12470 0.48 -0.6
2,423,855 + ascG NOLOHH_12470 0.50 +0.3
2,423,856 - ascG NOLOHH_12470 0.50 -1.6
2,423,954 + ascG NOLOHH_12470 0.60 -0.6
2,423,955 - ascG NOLOHH_12470 0.60 -1.5
2,423,955 - ascG NOLOHH_12470 0.60 +0.5
2,423,955 - ascG NOLOHH_12470 0.60 +0.4
2,423,978 + ascG NOLOHH_12470 0.62 -0.2
2,424,082 + ascG NOLOHH_12470 0.72 -0.3
2,424,082 + ascG NOLOHH_12470 0.72 +0.4
2,424,540 + -0.9
2,424,547 - -1.0
2,424,833 + hydN NOLOHH_12475 0.61 +1.1
2,424,833 + hydN NOLOHH_12475 0.61 +1.2
2,424,898 - hydN NOLOHH_12475 0.73 -0.7
2,424,898 - hydN NOLOHH_12475 0.73 +0.5
2,424,965 + hydN NOLOHH_12475 0.86 +0.3
2,424,966 - hydN NOLOHH_12475 0.86 +0.5
2,424,981 + hydN NOLOHH_12475 0.89 -2.0
2,424,982 - hydN NOLOHH_12475 0.89 +0.6
2,425,083 - +0.8
2,425,088 + -0.7
2,425,089 - +1.4
2,425,227 - -0.2
2,425,288 + -1.2
2,425,289 - +0.3
2,425,289 - +0.0
2,425,289 - -0.2
2,425,289 - -0.7
2,425,289 - -0.7
2,425,310 + -1.7
2,425,311 - +0.0
2,425,327 + -1.6

Or see this region's nucleotide sequence