Experiment: Bas69
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt mtnN and btuF overlap by 8 nucleotides btuF and yadS are separated by 37 nucleotides yadS and erpA are separated by 46 nucleotides erpA and yadW overlap by 8 nucleotides yadW and clcA are separated by 23 nucleotides
NOLOHH_02610: mtnN - 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase, at 430,520 to 431,218
mtnN
NOLOHH_02615: btuF - vitamin B12 ABC transporter substrate-binding protein BtuF, at 431,211 to 432,011
btuF
NOLOHH_02620: yadS - UPF0126 inner membrane protein YadS, at 432,049 to 432,672
yadS
NOLOHH_02625: erpA - iron-sulfur cluster insertion protein ErpA, at 432,719 to 433,063
erpA
NOLOHH_02630: yadW - Protein YadW, at 433,056 to 433,121
yadW
NOLOHH_02635: clcA - H(+)/Cl(-) exchange transporter ClcA, at 433,145 to 434,566
clcA
Position (kb) 432
433 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 431.216 kb on + strand at 431.216 kb on + strand at 431.216 kb on + strand at 431.217 kb on - strand at 431.217 kb on - strand at 431.358 kb on + strand, within btuF at 431.358 kb on + strand, within btuF at 431.359 kb on - strand, within btuF at 431.368 kb on - strand, within btuF at 431.474 kb on + strand, within btuF at 431.475 kb on - strand, within btuF at 431.567 kb on - strand, within btuF at 431.747 kb on - strand, within btuF at 431.747 kb on - strand, within btuF at 431.960 kb on + strand at 431.960 kb on + strand at 431.961 kb on - strand at 431.987 kb on + strand at 431.988 kb on - strand at 432.004 kb on + strand at 432.005 kb on - strand at 432.005 kb on - strand at 432.005 kb on - strand at 432.071 kb on + strand at 432.071 kb on + strand at 432.071 kb on + strand at 432.072 kb on - strand at 432.087 kb on - strand at 432.412 kb on - strand, within yadS at 432.510 kb on + strand, within yadS at 432.576 kb on + strand, within yadS at 432.576 kb on + strand, within yadS at 432.577 kb on - strand, within yadS at 432.633 kb on + strand at 432.633 kb on + strand at 432.634 kb on - strand at 432.692 kb on + strand at 432.720 kb on + strand at 432.721 kb on - strand at 432.721 kb on - strand at 432.721 kb on - strand at 432.721 kb on - strand at 432.721 kb on - strand at 432.721 kb on - strand at 432.727 kb on + strand at 433.110 kb on - strand, within yadW at 433.123 kb on - strand at 433.123 kb on - strand at 433.126 kb on + strand at 433.193 kb on - strand at 433.193 kb on - strand at 433.193 kb on - strand at 433.193 kb on - strand at 433.234 kb on - strand at 433.265 kb on + strand at 433.266 kb on - strand at 433.266 kb on - strand at 433.266 kb on - strand at 433.311 kb on + strand, within clcA at 433.311 kb on + strand, within clcA at 433.312 kb on - strand, within clcA at 433.312 kb on - strand, within clcA at 433.312 kb on - strand, within clcA at 433.533 kb on - strand, within clcA at 433.533 kb on - strand, within clcA at 433.533 kb on - strand, within clcA at 433.533 kb on - strand, within clcA at 433.533 kb on - strand, within clcA at 433.562 kb on - strand, within clcA at 433.562 kb on - strand, within clcA
Per-strain Table
Position Strand Gene LocusTag Fraction Bas69 remove 431,216 + +0.2 431,216 + -1.1 431,216 + -0.4 431,217 - -2.4 431,217 - +1.1 431,358 + btuF NOLOHH_02615 0.18 -1.1 431,358 + btuF NOLOHH_02615 0.18 +1.2 431,359 - btuF NOLOHH_02615 0.18 +0.6 431,368 - btuF NOLOHH_02615 0.20 +0.5 431,474 + btuF NOLOHH_02615 0.33 -1.9 431,475 - btuF NOLOHH_02615 0.33 +0.3 431,567 - btuF NOLOHH_02615 0.44 -0.7 431,747 - btuF NOLOHH_02615 0.67 -2.2 431,747 - btuF NOLOHH_02615 0.67 -0.7 431,960 + +0.6 431,960 + +1.3 431,961 - -1.1 431,987 + +0.4 431,988 - -0.3 432,004 + -1.5 432,005 - +2.9 432,005 - -0.1 432,005 - -0.4 432,071 + -0.7 432,071 + +2.1 432,071 + +0.9 432,072 - -0.9 432,087 - +0.9 432,412 - yadS NOLOHH_02620 0.58 +0.3 432,510 + yadS NOLOHH_02620 0.74 -1.1 432,576 + yadS NOLOHH_02620 0.84 -1.1 432,576 + yadS NOLOHH_02620 0.84 -1.4 432,577 - yadS NOLOHH_02620 0.85 -1.7 432,633 + +1.1 432,633 + +0.3 432,634 - +0.9 432,692 + -0.4 432,720 + +0.9 432,721 - -0.5 432,721 - -0.8 432,721 - -0.9 432,721 - +1.0 432,721 - -2.7 432,721 - +0.8 432,727 + -1.2 433,110 - yadW NOLOHH_02630 0.82 -1.5 433,123 - -0.4 433,123 - +0.2 433,126 + -0.4 433,193 - -0.2 433,193 - -1.2 433,193 - +0.6 433,193 - +1.2 433,234 - +2.2 433,265 + -0.1 433,266 - -1.1 433,266 - -1.1 433,266 - +0.5 433,311 + clcA NOLOHH_02635 0.12 +2.2 433,311 + clcA NOLOHH_02635 0.12 +0.1 433,312 - clcA NOLOHH_02635 0.12 -1.9 433,312 - clcA NOLOHH_02635 0.12 -2.4 433,312 - clcA NOLOHH_02635 0.12 -1.4 433,533 - clcA NOLOHH_02635 0.27 +0.6 433,533 - clcA NOLOHH_02635 0.27 -1.0 433,533 - clcA NOLOHH_02635 0.27 -1.0 433,533 - clcA NOLOHH_02635 0.27 -2.7 433,533 - clcA NOLOHH_02635 0.27 -1.1 433,562 - clcA NOLOHH_02635 0.29 -1.0 433,562 - clcA NOLOHH_02635 0.29 +0.4
Or see this region's nucleotide sequence