Strain Fitness in Escherichia coli ECOR27 around NOLOHH_17215

Experiment: Bas67

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntnimT and nimR are separated by 99 nucleotidesnimR and yeaL overlap by 44 nucleotidesyeaL and yeaK are separated by 274 nucleotidesyeaK and dgcJ are separated by 42 nucleotides NOLOHH_17205: nimT - 2-nitroimidazole transporter, at 3,375,068 to 3,376,249 nimT NOLOHH_17210: nimR - DNA-binding transcriptional regulator NimR, at 3,376,349 to 3,377,167 nimR NOLOHH_17215: yeaL - UPF0756 membrane protein CKO_01811, at 3,377,124 to 3,377,570 yeaL NOLOHH_17220: yeaK - mischarged aminoacyl-tRNA deacylase, at 3,377,845 to 3,378,348 yeaK NOLOHH_17225: dgcJ - diguanylate cyclase DgcJ, at 3,378,391 to 3,379,881 dgcJ Position (kb) 3377 3378Strain fitness (log2 ratio) -2 -1 0 1 2at 3376.249 kb on + strandat 3376.265 kb on - strandat 3376.479 kb on + strand, within nimRat 3376.485 kb on + strand, within nimRat 3376.485 kb on + strand, within nimRat 3377.065 kb on + strand, within nimRat 3377.065 kb on + strand, within nimRat 3377.066 kb on - strand, within nimRat 3377.068 kb on + strand, within nimRat 3377.071 kb on + strand, within nimRat 3377.071 kb on + strand, within nimRat 3377.111 kb on - strandat 3377.419 kb on - strand, within yeaLat 3377.605 kb on + strandat 3377.606 kb on - strandat 3377.842 kb on + strandat 3377.843 kb on - strandat 3377.843 kb on - strandat 3378.092 kb on - strand, within yeaKat 3378.093 kb on + strand, within yeaKat 3378.180 kb on + strand, within yeaKat 3378.247 kb on - strand, within yeaKat 3378.426 kb on - strandat 3378.428 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas67
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3,376,249 + -0.7
3,376,265 - -0.6
3,376,479 + nimR NOLOHH_17210 0.16 -0.7
3,376,485 + nimR NOLOHH_17210 0.17 -0.1
3,376,485 + nimR NOLOHH_17210 0.17 -0.1
3,377,065 + nimR NOLOHH_17210 0.87 +0.9
3,377,065 + nimR NOLOHH_17210 0.87 +1.3
3,377,066 - nimR NOLOHH_17210 0.88 +1.9
3,377,068 + nimR NOLOHH_17210 0.88 -0.3
3,377,071 + nimR NOLOHH_17210 0.88 -1.1
3,377,071 + nimR NOLOHH_17210 0.88 +1.3
3,377,111 - +1.3
3,377,419 - yeaL NOLOHH_17215 0.66 +0.3
3,377,605 + +0.3
3,377,606 - +1.0
3,377,842 + -1.3
3,377,843 - -2.5
3,377,843 - +2.3
3,378,092 - yeaK NOLOHH_17220 0.49 +0.8
3,378,093 + yeaK NOLOHH_17220 0.49 -1.7
3,378,180 + yeaK NOLOHH_17220 0.66 +0.3
3,378,247 - yeaK NOLOHH_17220 0.80 +0.3
3,378,426 - -0.7
3,378,428 - +2.6

Or see this region's nucleotide sequence