Experiment: Bas67
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt nudB and NOLOHH_16700 are separated by 28 nucleotides NOLOHH_16700 and NOLOHH_16705 overlap by 1 nucleotides NOLOHH_16705 and ruvC overlap by 1 nucleotides ruvC and NOLOHH_16715 are separated by 1 nucleotides
NOLOHH_16695: nudB - dihydroneopterin triphosphate diphosphatase, at 3,281,831 to 3,282,340
nudB
NOLOHH_16700: NOLOHH_16700 - putative transcriptional regulatory protein YebC, at 3,282,369 to 3,283,109
_16700
NOLOHH_16705: NOLOHH_16705 - Crossover junction endodeoxyribonuclease RuvC, at 3,283,109 to 3,283,144
_16705
NOLOHH_16710: ruvC - crossover junction endodeoxyribonuclease RuvC, at 3,283,144 to 3,283,665
ruvC
NOLOHH_16715: NOLOHH_16715 - YebB family permuted papain-like enzyme, at 3,283,667 to 3,284,269
_16715
Position (kb)
3283
3284 Strain fitness (log2 ratio)
-1
0
1
2 at 3282.147 kb on + strand, within nudB at 3282.178 kb on + strand, within nudB at 3282.248 kb on + strand, within nudB at 3282.286 kb on + strand, within nudB at 3282.324 kb on + strand at 3282.328 kb on + strand at 3282.446 kb on + strand, within NOLOHH_16700 at 3282.627 kb on + strand, within NOLOHH_16700 at 3282.636 kb on + strand, within NOLOHH_16700 at 3282.885 kb on + strand, within NOLOHH_16700 at 3283.668 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bas67 remove 3,282,147 + nudB NOLOHH_16695 0.62 -0.1 3,282,178 + nudB NOLOHH_16695 0.68 -0.7 3,282,248 + nudB NOLOHH_16695 0.82 +0.3 3,282,286 + nudB NOLOHH_16695 0.89 -1.0 3,282,324 + -1.0 3,282,328 + +1.3 3,282,446 + NOLOHH_16700 0.10 -0.7 3,282,627 + NOLOHH_16700 0.35 +1.8 3,282,636 + NOLOHH_16700 0.36 +0.7 3,282,885 + NOLOHH_16700 0.70 -0.7 3,283,668 + -0.7
Or see this region's nucleotide sequence