Strain Fitness in Escherichia coli ECOR27 around NOLOHH_11675

Experiment: Bas67

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntNOLOHH_11665 and escR overlap by 11 nucleotidesescR and NOLOHH_11675 are separated by 9 nucleotidesNOLOHH_11675 and sctT are separated by 75 nucleotidessctT and NOLOHH_11685 are separated by 8 nucleotides NOLOHH_11665: NOLOHH_11665 - YscQ/HrcQ family type III secretion apparatus protein, at 2,263,962 to 2,264,303 _11665 NOLOHH_11670: escR - EscR/YscR/HrcR family type III secretion system export apparatus protein, at 2,264,293 to 2,264,958 escR NOLOHH_11675: NOLOHH_11675 - EscS/YscS/HrcS family type III secretion system export apparatus protein, at 2,264,968 to 2,265,153 _11675 NOLOHH_11680: sctT - type III secretion system export apparatus subunit SctT, at 2,265,229 to 2,265,996 sctT NOLOHH_11685: NOLOHH_11685 - flagellar biosynthesis protein FlhB, at 2,266,005 to 2,266,736 _11685 Position (kb) 2264 2265 2266Strain fitness (log2 ratio) -1 0 1 2at 2264.397 kb on + strand, within escRat 2264.789 kb on + strand, within escRat 2265.488 kb on + strand, within sctTat 2265.747 kb on + strand, within sctT

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas67
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2,264,397 + escR NOLOHH_11670 0.16 -0.9
2,264,789 + escR NOLOHH_11670 0.74 +0.3
2,265,488 + sctT NOLOHH_11680 0.34 +1.9
2,265,747 + sctT NOLOHH_11680 0.67 -0.7

Or see this region's nucleotide sequence