Strain Fitness in Escherichia coli ECOR27 around NOLOHH_09740

Experiment: Bas67

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntrapZ and ptsN are separated by 45 nucleotidesptsN and NOLOHH_09735 are separated by 117 nucleotidesNOLOHH_09735 and rpoN are separated by 22 nucleotidesrpoN and lptB are separated by 47 nucleotideslptB and lptA are separated by 6 nucleotides NOLOHH_09725: rapZ - RNase adapter RapZ, at 1,877,562 to 1,878,416 rapZ NOLOHH_09730: ptsN - PTS IIA-like nitrogen regulatory protein PtsN, at 1,878,462 to 1,878,953 ptsN NOLOHH_09735: NOLOHH_09735 - Ribosome hibernation promoting factor, at 1,879,071 to 1,879,358 _09735 NOLOHH_09740: rpoN - RNA polymerase factor sigma-54, at 1,879,381 to 1,880,814 rpoN NOLOHH_09745: lptB - LPS export ABC transporter ATP-binding protein, at 1,880,862 to 1,881,587 lptB NOLOHH_09750: lptA - lipopolysaccharide ABC transporter substrate-binding protein LptA, at 1,881,594 to 1,882,151 lptA Position (kb) 1879 1880 1881Strain fitness (log2 ratio) -2 -1 0 1at 1878.412 kb on - strandat 1878.418 kb on + strandat 1878.418 kb on + strandat 1878.441 kb on - strandat 1878.460 kb on - strandat 1878.460 kb on - strandat 1878.463 kb on + strandat 1878.463 kb on + strandat 1878.464 kb on - strandat 1878.464 kb on - strandat 1878.464 kb on - strandat 1878.464 kb on - strandat 1878.479 kb on + strandat 1878.479 kb on + strandat 1878.603 kb on - strand, within ptsNat 1878.673 kb on - strand, within ptsNat 1878.717 kb on - strand, within ptsNat 1878.750 kb on - strand, within ptsNat 1878.795 kb on - strand, within ptsNat 1878.900 kb on - strand, within ptsNat 1878.924 kb on - strandat 1878.939 kb on - strandat 1878.964 kb on - strandat 1879.012 kb on - strandat 1879.057 kb on - strandat 1879.057 kb on - strandat 1879.057 kb on - strandat 1879.057 kb on - strandat 1879.095 kb on - strandat 1879.095 kb on - strandat 1879.095 kb on - strandat 1879.129 kb on - strand, within NOLOHH_09735at 1879.129 kb on - strand, within NOLOHH_09735at 1879.147 kb on + strand, within NOLOHH_09735at 1879.147 kb on + strand, within NOLOHH_09735at 1879.147 kb on + strand, within NOLOHH_09735at 1879.148 kb on - strand, within NOLOHH_09735at 1879.148 kb on - strand, within NOLOHH_09735at 1879.148 kb on - strand, within NOLOHH_09735at 1879.148 kb on - strand, within NOLOHH_09735at 1879.192 kb on - strand, within NOLOHH_09735at 1879.192 kb on - strand, within NOLOHH_09735at 1879.246 kb on + strand, within NOLOHH_09735at 1879.246 kb on + strand, within NOLOHH_09735at 1879.247 kb on - strand, within NOLOHH_09735at 1879.247 kb on - strand, within NOLOHH_09735at 1879.247 kb on - strand, within NOLOHH_09735at 1879.247 kb on - strand, within NOLOHH_09735at 1879.247 kb on - strand, within NOLOHH_09735at 1879.247 kb on - strand, within NOLOHH_09735at 1879.293 kb on - strand, within NOLOHH_09735at 1879.334 kb on + strandat 1879.335 kb on - strandat 1879.359 kb on - strandat 1879.359 kb on - strandat 1880.837 kb on + strandat 1880.856 kb on - strandat 1880.861 kb on - strandat 1881.600 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas67
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1,878,412 - +0.4
1,878,418 + -0.6
1,878,418 + +1.4
1,878,441 - -1.6
1,878,460 - +0.2
1,878,460 - -0.3
1,878,463 + -0.6
1,878,463 + +1.4
1,878,464 - +0.0
1,878,464 - -0.6
1,878,464 - +0.7
1,878,464 - +0.0
1,878,479 + +0.4
1,878,479 + +0.4
1,878,603 - ptsN NOLOHH_09730 0.29 -0.6
1,878,673 - ptsN NOLOHH_09730 0.43 +0.7
1,878,717 - ptsN NOLOHH_09730 0.52 +0.4
1,878,750 - ptsN NOLOHH_09730 0.59 +0.4
1,878,795 - ptsN NOLOHH_09730 0.68 -0.6
1,878,900 - ptsN NOLOHH_09730 0.89 +0.4
1,878,924 - +1.4
1,878,939 - +0.0
1,878,964 - -0.8
1,879,012 - +0.0
1,879,057 - +0.8
1,879,057 - +0.3
1,879,057 - -1.8
1,879,057 - +1.0
1,879,095 - -0.2
1,879,095 - -0.7
1,879,095 - +0.3
1,879,129 - NOLOHH_09735 0.20 -1.2
1,879,129 - NOLOHH_09735 0.20 +1.0
1,879,147 + NOLOHH_09735 0.26 +0.4
1,879,147 + NOLOHH_09735 0.26 +1.0
1,879,147 + NOLOHH_09735 0.26 -1.9
1,879,148 - NOLOHH_09735 0.27 -1.4
1,879,148 - NOLOHH_09735 0.27 +0.8
1,879,148 - NOLOHH_09735 0.27 -1.0
1,879,148 - NOLOHH_09735 0.27 -0.2
1,879,192 - NOLOHH_09735 0.42 -0.2
1,879,192 - NOLOHH_09735 0.42 -0.7
1,879,246 + NOLOHH_09735 0.61 -1.2
1,879,246 + NOLOHH_09735 0.61 +0.4
1,879,247 - NOLOHH_09735 0.61 -0.2
1,879,247 - NOLOHH_09735 0.61 -0.5
1,879,247 - NOLOHH_09735 0.61 +0.4
1,879,247 - NOLOHH_09735 0.61 -0.1
1,879,247 - NOLOHH_09735 0.61 +0.2
1,879,247 - NOLOHH_09735 0.61 -0.3
1,879,293 - NOLOHH_09735 0.77 -0.6
1,879,334 + -2.4
1,879,335 - -0.7
1,879,359 - -1.9
1,879,359 - -0.5
1,880,837 + +0.4
1,880,856 - -0.7
1,880,861 - +1.0
1,881,600 - +1.4

Or see this region's nucleotide sequence