Experiment: Bas67
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rapZ and ptsN are separated by 45 nucleotides ptsN and NOLOHH_09735 are separated by 117 nucleotides NOLOHH_09735 and rpoN are separated by 22 nucleotides rpoN and lptB are separated by 47 nucleotides lptB and lptA are separated by 6 nucleotides
NOLOHH_09725: rapZ - RNase adapter RapZ, at 1,877,562 to 1,878,416
rapZ
NOLOHH_09730: ptsN - PTS IIA-like nitrogen regulatory protein PtsN, at 1,878,462 to 1,878,953
ptsN
NOLOHH_09735: NOLOHH_09735 - Ribosome hibernation promoting factor, at 1,879,071 to 1,879,358
_09735
NOLOHH_09740: rpoN - RNA polymerase factor sigma-54, at 1,879,381 to 1,880,814
rpoN
NOLOHH_09745: lptB - LPS export ABC transporter ATP-binding protein, at 1,880,862 to 1,881,587
lptB
NOLOHH_09750: lptA - lipopolysaccharide ABC transporter substrate-binding protein LptA, at 1,881,594 to 1,882,151
lptA
Position (kb)
1879
1880
1881 Strain fitness (log2 ratio)
-2
-1
0
1 at 1878.412 kb on - strand at 1878.418 kb on + strand at 1878.418 kb on + strand at 1878.441 kb on - strand at 1878.460 kb on - strand at 1878.460 kb on - strand at 1878.463 kb on + strand at 1878.463 kb on + strand at 1878.464 kb on - strand at 1878.464 kb on - strand at 1878.464 kb on - strand at 1878.464 kb on - strand at 1878.479 kb on + strand at 1878.479 kb on + strand at 1878.603 kb on - strand, within ptsN at 1878.673 kb on - strand, within ptsN at 1878.717 kb on - strand, within ptsN at 1878.750 kb on - strand, within ptsN at 1878.795 kb on - strand, within ptsN at 1878.900 kb on - strand, within ptsN at 1878.924 kb on - strand at 1878.939 kb on - strand at 1878.964 kb on - strand at 1879.012 kb on - strand at 1879.057 kb on - strand at 1879.057 kb on - strand at 1879.057 kb on - strand at 1879.057 kb on - strand at 1879.095 kb on - strand at 1879.095 kb on - strand at 1879.095 kb on - strand at 1879.129 kb on - strand, within NOLOHH_09735 at 1879.129 kb on - strand, within NOLOHH_09735 at 1879.147 kb on + strand, within NOLOHH_09735 at 1879.147 kb on + strand, within NOLOHH_09735 at 1879.147 kb on + strand, within NOLOHH_09735 at 1879.148 kb on - strand, within NOLOHH_09735 at 1879.148 kb on - strand, within NOLOHH_09735 at 1879.148 kb on - strand, within NOLOHH_09735 at 1879.148 kb on - strand, within NOLOHH_09735 at 1879.192 kb on - strand, within NOLOHH_09735 at 1879.192 kb on - strand, within NOLOHH_09735 at 1879.246 kb on + strand, within NOLOHH_09735 at 1879.246 kb on + strand, within NOLOHH_09735 at 1879.247 kb on - strand, within NOLOHH_09735 at 1879.247 kb on - strand, within NOLOHH_09735 at 1879.247 kb on - strand, within NOLOHH_09735 at 1879.247 kb on - strand, within NOLOHH_09735 at 1879.247 kb on - strand, within NOLOHH_09735 at 1879.247 kb on - strand, within NOLOHH_09735 at 1879.293 kb on - strand, within NOLOHH_09735 at 1879.334 kb on + strand at 1879.335 kb on - strand at 1879.359 kb on - strand at 1879.359 kb on - strand at 1880.837 kb on + strand at 1880.856 kb on - strand at 1880.861 kb on - strand at 1881.600 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bas67 remove 1,878,412 - +0.4 1,878,418 + -0.6 1,878,418 + +1.4 1,878,441 - -1.6 1,878,460 - +0.2 1,878,460 - -0.3 1,878,463 + -0.6 1,878,463 + +1.4 1,878,464 - +0.0 1,878,464 - -0.6 1,878,464 - +0.7 1,878,464 - +0.0 1,878,479 + +0.4 1,878,479 + +0.4 1,878,603 - ptsN NOLOHH_09730 0.29 -0.6 1,878,673 - ptsN NOLOHH_09730 0.43 +0.7 1,878,717 - ptsN NOLOHH_09730 0.52 +0.4 1,878,750 - ptsN NOLOHH_09730 0.59 +0.4 1,878,795 - ptsN NOLOHH_09730 0.68 -0.6 1,878,900 - ptsN NOLOHH_09730 0.89 +0.4 1,878,924 - +1.4 1,878,939 - +0.0 1,878,964 - -0.8 1,879,012 - +0.0 1,879,057 - +0.8 1,879,057 - +0.3 1,879,057 - -1.8 1,879,057 - +1.0 1,879,095 - -0.2 1,879,095 - -0.7 1,879,095 - +0.3 1,879,129 - NOLOHH_09735 0.20 -1.2 1,879,129 - NOLOHH_09735 0.20 +1.0 1,879,147 + NOLOHH_09735 0.26 +0.4 1,879,147 + NOLOHH_09735 0.26 +1.0 1,879,147 + NOLOHH_09735 0.26 -1.9 1,879,148 - NOLOHH_09735 0.27 -1.4 1,879,148 - NOLOHH_09735 0.27 +0.8 1,879,148 - NOLOHH_09735 0.27 -1.0 1,879,148 - NOLOHH_09735 0.27 -0.2 1,879,192 - NOLOHH_09735 0.42 -0.2 1,879,192 - NOLOHH_09735 0.42 -0.7 1,879,246 + NOLOHH_09735 0.61 -1.2 1,879,246 + NOLOHH_09735 0.61 +0.4 1,879,247 - NOLOHH_09735 0.61 -0.2 1,879,247 - NOLOHH_09735 0.61 -0.5 1,879,247 - NOLOHH_09735 0.61 +0.4 1,879,247 - NOLOHH_09735 0.61 -0.1 1,879,247 - NOLOHH_09735 0.61 +0.2 1,879,247 - NOLOHH_09735 0.61 -0.3 1,879,293 - NOLOHH_09735 0.77 -0.6 1,879,334 + -2.4 1,879,335 - -0.7 1,879,359 - -1.9 1,879,359 - -0.5 1,880,837 + +0.4 1,880,856 - -0.7 1,880,861 - +1.0 1,881,600 - +1.4
Or see this region's nucleotide sequence