Strain Fitness in Escherichia coli ECOR27 around NOLOHH_02500

Experiment: Bas67

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntlpxB and lpxA overlap by 1 nucleotideslpxA and fabZ are separated by 3 nucleotidesfabZ and lpxD are separated by 104 nucleotides NOLOHH_02490: lpxB - lipid-A-disaccharide synthase, at 405,893 to 407,041 lpxB NOLOHH_02495: lpxA - acyl-ACP--UDP-N-acetylglucosamine O-acyltransferase, at 407,041 to 407,829 lpxA NOLOHH_02500: fabZ - 3-hydroxyacyl-ACP dehydratase FabZ, at 407,833 to 408,288 fabZ NOLOHH_02505: lpxD - UDP-3-O-(3-hydroxymyristoyl)glucosamine N-acyltransferase, at 408,393 to 409,418 lpxD Position (kb) 407 408 409Strain fitness (log2 ratio) -1 0 1at 408.327 kb on - strandat 408.348 kb on - strandat 408.394 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas67
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408,327 - +1.7
408,348 - -0.6
408,394 + +1.0

Or see this region's nucleotide sequence