Strain Fitness in Escherichia coli ECOR27 around NOLOHH_01365

Experiment: Bas67

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntthiI and xseB are separated by 205 nucleotidesxseB and ispA overlap by 1 nucleotidesispA and dxs are separated by 24 nucleotides NOLOHH_01355: thiI - tRNA sulfurtransferase, at 163,209 to 164,657 thiI NOLOHH_01360: xseB - exodeoxyribonuclease VII small subunit, at 164,863 to 165,105 xseB NOLOHH_01365: ispA - (2E,6E)-farnesyl diphosphate synthase, at 165,105 to 166,004 ispA NOLOHH_01370: dxs - 1-deoxy-D-xylulose-5-phosphate synthase, at 166,029 to 167,891 dxs Position (kb) 165 166 167Strain fitness (log2 ratio) -2 -1 0 1 2at 164.252 kb on + strand, within thiIat 164.253 kb on - strand, within thiIat 164.278 kb on + strand, within thiIat 164.433 kb on - strand, within thiIat 164.549 kb on - strandat 164.557 kb on - strandat 164.590 kb on - strandat 164.592 kb on - strandat 164.648 kb on + strandat 164.684 kb on + strandat 164.695 kb on + strandat 164.695 kb on + strandat 164.696 kb on - strandat 164.696 kb on - strandat 164.696 kb on - strandat 164.696 kb on - strandat 164.696 kb on - strandat 164.696 kb on - strandat 164.696 kb on - strandat 164.708 kb on - strandat 164.747 kb on + strandat 164.748 kb on - strandat 164.749 kb on + strandat 164.750 kb on - strandat 164.750 kb on - strandat 164.756 kb on - strandat 164.783 kb on + strandat 164.784 kb on - strandat 164.797 kb on - strandat 164.797 kb on - strandat 164.803 kb on + strandat 165.087 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas67
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164,252 + thiI NOLOHH_01355 0.72 -1.2
164,253 - thiI NOLOHH_01355 0.72 +0.7
164,278 + thiI NOLOHH_01355 0.74 -1.6
164,433 - thiI NOLOHH_01355 0.84 +1.3
164,549 - +0.1
164,557 - -0.5
164,590 - +0.2
164,592 - +0.6
164,648 + +2.4
164,684 + -0.6
164,695 + +1.4
164,695 + +0.1
164,696 - +2.0
164,696 - +1.6
164,696 - -0.3
164,696 - -1.0
164,696 - -0.2
164,696 - -0.4
164,696 - +0.2
164,708 - +0.1
164,747 + +0.7
164,748 - +1.4
164,749 + +0.6
164,750 - -1.8
164,750 - -0.3
164,756 - +0.4
164,783 + -0.2
164,784 - +0.0
164,797 - +0.6
164,797 - +0.1
164,803 + +0.3
165,087 + +0.4

Or see this region's nucleotide sequence