Experiment: R2A_PIPES with Aluminum chloride 0.28125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ribD and nrdR are separated by 95 nucleotides nrdR and glyA are separated by 96 nucleotides
OKGIIK_11430: ribD - bifunctional diaminohydroxyphosphoribosylaminopyrimidine deaminase/5-amino-6-(5-phosphoribosylamino)uracil reductase RibD, at 2,454,344 to 2,455,462
ribD
OKGIIK_11435: nrdR - transcriptional regulator NrdR, at 2,455,558 to 2,456,070
nrdR
OKGIIK_11440: glyA - serine hydroxymethyltransferase, at 2,456,167 to 2,457,435
glyA
Position (kb)
2455
2456
2457 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2455.450 kb on - strand at 2455.479 kb on - strand at 2455.479 kb on - strand at 2455.479 kb on - strand at 2455.479 kb on - strand at 2455.479 kb on - strand at 2455.479 kb on - strand at 2455.545 kb on + strand at 2455.545 kb on + strand at 2455.545 kb on + strand at 2455.545 kb on + strand at 2455.545 kb on + strand at 2455.679 kb on + strand, within nrdR at 2455.679 kb on + strand, within nrdR at 2455.680 kb on - strand, within nrdR at 2455.700 kb on + strand, within nrdR at 2455.701 kb on - strand, within nrdR at 2455.701 kb on - strand, within nrdR at 2455.701 kb on - strand, within nrdR at 2455.701 kb on - strand, within nrdR at 2455.701 kb on - strand, within nrdR at 2455.808 kb on - strand, within nrdR at 2455.808 kb on - strand, within nrdR at 2456.071 kb on - strand at 2456.071 kb on - strand at 2456.071 kb on - strand at 2456.071 kb on - strand at 2456.088 kb on + strand at 2456.089 kb on - strand at 2456.089 kb on - strand at 2456.174 kb on + strand at 2456.175 kb on - strand at 2456.300 kb on + strand, within glyA at 2456.300 kb on + strand, within glyA at 2456.300 kb on + strand, within glyA at 2456.301 kb on - strand, within glyA at 2456.301 kb on - strand, within glyA at 2456.301 kb on - strand, within glyA at 2456.301 kb on - strand, within glyA at 2456.310 kb on + strand, within glyA at 2456.561 kb on + strand, within glyA at 2456.561 kb on + strand, within glyA at 2456.561 kb on + strand, within glyA at 2456.561 kb on + strand, within glyA at 2456.561 kb on + strand, within glyA at 2456.562 kb on - strand, within glyA at 2456.562 kb on - strand, within glyA at 2456.562 kb on - strand, within glyA at 2456.562 kb on - strand, within glyA at 2456.849 kb on + strand, within glyA at 2456.849 kb on + strand, within glyA at 2456.850 kb on - strand, within glyA at 2456.850 kb on - strand, within glyA at 2456.850 kb on - strand, within glyA at 2456.850 kb on - strand, within glyA at 2456.906 kb on + strand, within glyA at 2456.907 kb on - strand, within glyA at 2456.972 kb on + strand, within glyA at 2456.973 kb on - strand, within glyA at 2457.002 kb on + strand, within glyA at 2457.002 kb on + strand, within glyA at 2457.002 kb on + strand, within glyA at 2457.003 kb on - strand, within glyA at 2457.003 kb on - strand, within glyA
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Aluminum chloride 0.28125 mM remove 2,455,450 - +0.4 2,455,479 - +0.0 2,455,479 - -0.0 2,455,479 - -0.9 2,455,479 - +2.7 2,455,479 - +0.2 2,455,479 - -2.0 2,455,545 + +2.6 2,455,545 + +0.1 2,455,545 + +0.0 2,455,545 + +1.3 2,455,545 + -0.3 2,455,679 + nrdR OKGIIK_11435 0.24 -0.5 2,455,679 + nrdR OKGIIK_11435 0.24 +1.0 2,455,680 - nrdR OKGIIK_11435 0.24 -1.3 2,455,700 + nrdR OKGIIK_11435 0.28 -0.6 2,455,701 - nrdR OKGIIK_11435 0.28 +0.9 2,455,701 - nrdR OKGIIK_11435 0.28 +0.0 2,455,701 - nrdR OKGIIK_11435 0.28 -1.1 2,455,701 - nrdR OKGIIK_11435 0.28 -0.8 2,455,701 - nrdR OKGIIK_11435 0.28 +1.3 2,455,808 - nrdR OKGIIK_11435 0.49 +0.3 2,455,808 - nrdR OKGIIK_11435 0.49 -1.1 2,456,071 - +0.0 2,456,071 - +1.0 2,456,071 - -1.1 2,456,071 - -0.9 2,456,088 + -0.3 2,456,089 - -2.3 2,456,089 - -0.3 2,456,174 + +2.0 2,456,175 - -1.1 2,456,300 + glyA OKGIIK_11440 0.10 +1.6 2,456,300 + glyA OKGIIK_11440 0.10 -0.3 2,456,300 + glyA OKGIIK_11440 0.10 -1.3 2,456,301 - glyA OKGIIK_11440 0.11 +0.0 2,456,301 - glyA OKGIIK_11440 0.11 -0.6 2,456,301 - glyA OKGIIK_11440 0.11 +0.0 2,456,301 - glyA OKGIIK_11440 0.11 +0.6 2,456,310 + glyA OKGIIK_11440 0.11 +0.4 2,456,561 + glyA OKGIIK_11440 0.31 +0.4 2,456,561 + glyA OKGIIK_11440 0.31 +0.4 2,456,561 + glyA OKGIIK_11440 0.31 -1.0 2,456,561 + glyA OKGIIK_11440 0.31 -0.1 2,456,561 + glyA OKGIIK_11440 0.31 -0.3 2,456,562 - glyA OKGIIK_11440 0.31 +0.3 2,456,562 - glyA OKGIIK_11440 0.31 -2.1 2,456,562 - glyA OKGIIK_11440 0.31 +0.8 2,456,562 - glyA OKGIIK_11440 0.31 +0.4 2,456,849 + glyA OKGIIK_11440 0.54 -0.3 2,456,849 + glyA OKGIIK_11440 0.54 -1.6 2,456,850 - glyA OKGIIK_11440 0.54 -0.6 2,456,850 - glyA OKGIIK_11440 0.54 +0.6 2,456,850 - glyA OKGIIK_11440 0.54 -0.8 2,456,850 - glyA OKGIIK_11440 0.54 -0.1 2,456,906 + glyA OKGIIK_11440 0.58 +1.2 2,456,907 - glyA OKGIIK_11440 0.58 -1.0 2,456,972 + glyA OKGIIK_11440 0.63 -2.1 2,456,973 - glyA OKGIIK_11440 0.64 -1.1 2,457,002 + glyA OKGIIK_11440 0.66 -0.6 2,457,002 + glyA OKGIIK_11440 0.66 -1.0 2,457,002 + glyA OKGIIK_11440 0.66 -0.6 2,457,003 - glyA OKGIIK_11440 0.66 -0.3 2,457,003 - glyA OKGIIK_11440 0.66 -0.1
Or see this region's nucleotide sequence