Experiment: R2A_PIPES with Aluminum chloride 0.28125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt cusA and OKGIIK_06275 overlap by 4 nucleotides OKGIIK_06275 and OKGIIK_06280 are separated by 133 nucleotides OKGIIK_06280 and OKGIIK_06285 overlap by 1 nucleotides
OKGIIK_06270: cusA - CusA/CzcA family heavy metal efflux RND transporter, at 1,377,372 to 1,380,506
cusA
OKGIIK_06275: OKGIIK_06275 - Transcriptional regulator, at 1,380,503 to 1,380,832
_06275
OKGIIK_06280: OKGIIK_06280 - Cobalt-zinc-cadmium resistance protein CzcD, at 1,380,966 to 1,381,586
_06280
OKGIIK_06285: OKGIIK_06285 - Cation diffusion facilitator family transporter, at 1,381,586 to 1,382,491
_06285
Position (kb)
1380
1381 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1379.808 kb on + strand, within cusA at 1379.809 kb on - strand, within cusA at 1379.841 kb on + strand, within cusA at 1379.943 kb on + strand, within cusA at 1379.943 kb on + strand, within cusA at 1379.944 kb on - strand, within cusA at 1379.944 kb on - strand, within cusA at 1379.944 kb on - strand, within cusA at 1380.042 kb on + strand, within cusA at 1380.042 kb on + strand, within cusA at 1380.043 kb on - strand, within cusA at 1380.061 kb on + strand, within cusA at 1380.105 kb on - strand, within cusA at 1380.141 kb on + strand, within cusA at 1380.141 kb on + strand, within cusA at 1380.141 kb on + strand, within cusA at 1380.141 kb on + strand, within cusA at 1380.142 kb on - strand, within cusA at 1380.142 kb on - strand, within cusA at 1380.142 kb on - strand, within cusA at 1380.450 kb on + strand at 1380.513 kb on + strand at 1380.514 kb on - strand at 1380.514 kb on - strand at 1380.581 kb on + strand, within OKGIIK_06275 at 1380.641 kb on + strand, within OKGIIK_06275 at 1380.641 kb on + strand, within OKGIIK_06275 at 1380.641 kb on + strand, within OKGIIK_06275 at 1380.641 kb on + strand, within OKGIIK_06275 at 1380.642 kb on - strand, within OKGIIK_06275 at 1380.642 kb on - strand, within OKGIIK_06275 at 1380.848 kb on + strand at 1380.849 kb on - strand at 1380.897 kb on + strand at 1380.927 kb on + strand at 1380.927 kb on + strand at 1380.927 kb on + strand at 1380.974 kb on + strand at 1380.974 kb on + strand at 1380.974 kb on + strand at 1380.974 kb on + strand at 1380.975 kb on - strand at 1380.975 kb on - strand at 1380.975 kb on - strand at 1380.975 kb on - strand at 1381.061 kb on + strand, within OKGIIK_06280 at 1381.062 kb on - strand, within OKGIIK_06280 at 1381.092 kb on + strand, within OKGIIK_06280 at 1381.092 kb on + strand, within OKGIIK_06280 at 1381.092 kb on + strand, within OKGIIK_06280 at 1381.150 kb on + strand, within OKGIIK_06280 at 1381.152 kb on + strand, within OKGIIK_06280 at 1381.152 kb on + strand, within OKGIIK_06280 at 1381.152 kb on + strand, within OKGIIK_06280 at 1381.152 kb on + strand, within OKGIIK_06280 at 1381.153 kb on - strand, within OKGIIK_06280 at 1381.153 kb on - strand, within OKGIIK_06280 at 1381.153 kb on - strand, within OKGIIK_06280 at 1381.153 kb on - strand, within OKGIIK_06280 at 1381.566 kb on + strand at 1381.566 kb on + strand at 1381.567 kb on - strand at 1381.567 kb on - strand at 1381.567 kb on - strand at 1381.663 kb on + strand at 1381.663 kb on + strand at 1381.663 kb on + strand at 1381.663 kb on + strand at 1381.663 kb on + strand at 1381.663 kb on + strand at 1381.664 kb on - strand at 1381.664 kb on - strand at 1381.664 kb on - strand at 1381.664 kb on - strand at 1381.664 kb on - strand at 1381.664 kb on - strand at 1381.664 kb on - strand at 1381.699 kb on + strand, within OKGIIK_06285 at 1381.699 kb on + strand, within OKGIIK_06285 at 1381.700 kb on - strand, within OKGIIK_06285 at 1381.700 kb on - strand, within OKGIIK_06285 at 1381.817 kb on + strand, within OKGIIK_06285 at 1381.817 kb on + strand, within OKGIIK_06285 at 1381.817 kb on + strand, within OKGIIK_06285 at 1381.817 kb on + strand, within OKGIIK_06285 at 1381.817 kb on + strand, within OKGIIK_06285 at 1381.817 kb on + strand, within OKGIIK_06285 at 1381.817 kb on + strand, within OKGIIK_06285 at 1381.817 kb on + strand, within OKGIIK_06285 at 1381.818 kb on - strand, within OKGIIK_06285 at 1381.818 kb on - strand, within OKGIIK_06285 at 1381.818 kb on - strand, within OKGIIK_06285 at 1381.818 kb on - strand, within OKGIIK_06285 at 1381.818 kb on - strand, within OKGIIK_06285 at 1381.818 kb on - strand, within OKGIIK_06285 at 1381.818 kb on - strand, within OKGIIK_06285 at 1381.818 kb on - strand, within OKGIIK_06285 at 1381.818 kb on - strand, within OKGIIK_06285 at 1381.823 kb on + strand, within OKGIIK_06285
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Aluminum chloride 0.28125 mM remove 1,379,808 + cusA OKGIIK_06270 0.78 -0.5 1,379,809 - cusA OKGIIK_06270 0.78 -2.4 1,379,841 + cusA OKGIIK_06270 0.79 +0.5 1,379,943 + cusA OKGIIK_06270 0.82 -0.6 1,379,943 + cusA OKGIIK_06270 0.82 -0.3 1,379,944 - cusA OKGIIK_06270 0.82 +1.9 1,379,944 - cusA OKGIIK_06270 0.82 -0.6 1,379,944 - cusA OKGIIK_06270 0.82 -1.6 1,380,042 + cusA OKGIIK_06270 0.85 -1.7 1,380,042 + cusA OKGIIK_06270 0.85 -1.8 1,380,043 - cusA OKGIIK_06270 0.85 -0.2 1,380,061 + cusA OKGIIK_06270 0.86 -0.6 1,380,105 - cusA OKGIIK_06270 0.87 +0.5 1,380,141 + cusA OKGIIK_06270 0.88 -0.1 1,380,141 + cusA OKGIIK_06270 0.88 -1.3 1,380,141 + cusA OKGIIK_06270 0.88 +0.4 1,380,141 + cusA OKGIIK_06270 0.88 -0.5 1,380,142 - cusA OKGIIK_06270 0.88 -0.8 1,380,142 - cusA OKGIIK_06270 0.88 -0.3 1,380,142 - cusA OKGIIK_06270 0.88 +0.7 1,380,450 + -0.1 1,380,513 + -0.6 1,380,514 - +0.7 1,380,514 - -1.5 1,380,581 + OKGIIK_06275 0.24 -0.3 1,380,641 + OKGIIK_06275 0.42 -1.1 1,380,641 + OKGIIK_06275 0.42 -1.5 1,380,641 + OKGIIK_06275 0.42 -2.0 1,380,641 + OKGIIK_06275 0.42 -0.9 1,380,642 - OKGIIK_06275 0.42 -1.6 1,380,642 - OKGIIK_06275 0.42 -0.3 1,380,848 + -2.1 1,380,849 - -0.6 1,380,897 + -0.3 1,380,927 + -1.0 1,380,927 + -1.6 1,380,927 + -0.3 1,380,974 + -1.1 1,380,974 + +0.7 1,380,974 + -0.3 1,380,974 + -0.8 1,380,975 - +0.1 1,380,975 - -0.4 1,380,975 - -0.3 1,380,975 - -1.5 1,381,061 + OKGIIK_06280 0.15 +0.3 1,381,062 - OKGIIK_06280 0.15 -2.9 1,381,092 + OKGIIK_06280 0.20 -0.9 1,381,092 + OKGIIK_06280 0.20 -0.3 1,381,092 + OKGIIK_06280 0.20 +0.7 1,381,150 + OKGIIK_06280 0.30 -0.6 1,381,152 + OKGIIK_06280 0.30 +0.1 1,381,152 + OKGIIK_06280 0.30 +0.5 1,381,152 + OKGIIK_06280 0.30 +0.1 1,381,152 + OKGIIK_06280 0.30 -0.6 1,381,153 - OKGIIK_06280 0.30 -1.1 1,381,153 - OKGIIK_06280 0.30 -1.1 1,381,153 - OKGIIK_06280 0.30 -0.3 1,381,153 - OKGIIK_06280 0.30 -1.0 1,381,566 + +0.2 1,381,566 + +0.3 1,381,567 - -0.7 1,381,567 - +1.2 1,381,567 - -1.4 1,381,663 + -1.6 1,381,663 + +0.4 1,381,663 + -0.8 1,381,663 + +1.7 1,381,663 + -1.1 1,381,663 + -1.1 1,381,664 - +0.9 1,381,664 - -1.8 1,381,664 - -0.0 1,381,664 - +1.4 1,381,664 - -0.3 1,381,664 - +0.7 1,381,664 - -1.1 1,381,699 + OKGIIK_06285 0.12 -0.9 1,381,699 + OKGIIK_06285 0.12 -1.3 1,381,700 - OKGIIK_06285 0.13 -0.5 1,381,700 - OKGIIK_06285 0.13 +0.7 1,381,817 + OKGIIK_06285 0.25 -0.3 1,381,817 + OKGIIK_06285 0.25 -1.3 1,381,817 + OKGIIK_06285 0.25 +0.0 1,381,817 + OKGIIK_06285 0.25 -1.3 1,381,817 + OKGIIK_06285 0.25 +1.3 1,381,817 + OKGIIK_06285 0.25 -0.3 1,381,817 + OKGIIK_06285 0.25 -0.2 1,381,817 + OKGIIK_06285 0.25 -0.9 1,381,818 - OKGIIK_06285 0.26 -2.6 1,381,818 - OKGIIK_06285 0.26 -1.6 1,381,818 - OKGIIK_06285 0.26 -1.3 1,381,818 - OKGIIK_06285 0.26 -0.6 1,381,818 - OKGIIK_06285 0.26 -1.3 1,381,818 - OKGIIK_06285 0.26 -1.1 1,381,818 - OKGIIK_06285 0.26 +0.4 1,381,818 - OKGIIK_06285 0.26 -2.1 1,381,818 - OKGIIK_06285 0.26 -0.9 1,381,823 + OKGIIK_06285 0.26 -1.1
Or see this region's nucleotide sequence