Strain Fitness in Escherichia coli BW25113 around b2553

Experiment: Putrescine (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nthmp and glnB are separated by 44 nucleotidesglnB and yfhA are separated by 60 nucleotides b2552: hmp - fused nitric oxide dioxygenase/dihydropteridine reductase 2 (NCBI), at 2,683,857 to 2,685,047 hmp b2553: glnB - regulatory protein P-II for glutamine synthetase (NCBI), at 2,685,092 to 2,685,430 glnB b2554: yfhA - predicted DNA-binding response regulator in two-component system (NCBI), at 2,685,491 to 2,686,825 yfhA Position (kb) 2685 2686Strain fitness (log2 ratio) -1 0 1at 2684.113 kb on - strand, within hmpat 2684.136 kb on + strand, within hmpat 2684.136 kb on + strand, within hmpat 2684.146 kb on - strand, within hmpat 2684.146 kb on - strand, within hmpat 2684.146 kb on - strand, within hmpat 2684.146 kb on - strand, within hmpat 2684.160 kb on - strand, within hmpat 2684.160 kb on - strand, within hmpat 2684.191 kb on - strand, within hmpat 2684.191 kb on - strand, within hmpat 2684.207 kb on + strand, within hmpat 2684.222 kb on + strand, within hmpat 2684.222 kb on + strand, within hmpat 2684.330 kb on + strand, within hmpat 2684.338 kb on - strand, within hmpat 2684.338 kb on - strand, within hmpat 2684.405 kb on - strand, within hmpat 2684.476 kb on - strand, within hmpat 2684.721 kb on + strand, within hmpat 2684.789 kb on + strand, within hmpat 2684.789 kb on + strand, within hmpat 2684.827 kb on - strand, within hmpat 2684.830 kb on - strand, within hmpat 2684.970 kb on + strandat 2684.970 kb on + strandat 2685.032 kb on + strandat 2685.047 kb on - strandat 2685.079 kb on + strandat 2685.107 kb on + strandat 2685.107 kb on + strandat 2685.120 kb on + strandat 2685.120 kb on + strandat 2685.223 kb on - strand, within glnBat 2685.273 kb on + strand, within glnBat 2685.273 kb on + strand, within glnBat 2685.281 kb on - strand, within glnBat 2685.288 kb on + strand, within glnBat 2685.311 kb on - strand, within glnBat 2685.311 kb on - strand, within glnBat 2685.311 kb on - strand, within glnBat 2685.311 kb on - strand, within glnBat 2685.371 kb on - strand, within glnBat 2685.488 kb on + strandat 2685.488 kb on + strandat 2685.525 kb on - strandat 2685.682 kb on + strand, within yfhAat 2685.703 kb on + strand, within yfhAat 2685.703 kb on + strand, within yfhAat 2685.725 kb on + strand, within yfhAat 2685.821 kb on - strand, within yfhAat 2685.821 kb on - strand, within yfhAat 2685.863 kb on - strand, within yfhAat 2685.885 kb on - strand, within yfhAat 2685.885 kb on - strand, within yfhAat 2685.897 kb on - strand, within yfhAat 2686.064 kb on + strand, within yfhAat 2686.079 kb on + strand, within yfhAat 2686.089 kb on + strandat 2686.091 kb on + strand, within yfhAat 2686.091 kb on + strand, within yfhAat 2686.158 kb on - strand, within yfhAat 2686.162 kb on - strand, within yfhAat 2686.250 kb on + strand, within yfhAat 2686.282 kb on + strand, within yfhAat 2686.294 kb on - strand, within yfhAat 2686.323 kb on + strand, within yfhAat 2686.323 kb on + strand, within yfhAat 2686.331 kb on - strand, within yfhAat 2686.425 kb on - strand, within yfhA

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Per-strain Table

Position Strand Gene LocusTag Fraction Putrescine (N)
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2,684,113 - hmp b2552 0.21 -0.5
2,684,136 + hmp b2552 0.23 +0.1
2,684,136 + hmp b2552 0.23 +1.0
2,684,146 - hmp b2552 0.24 +0.6
2,684,146 - hmp b2552 0.24 -0.8
2,684,146 - hmp b2552 0.24 -0.2
2,684,146 - hmp b2552 0.24 -1.1
2,684,160 - hmp b2552 0.25 -1.0
2,684,160 - hmp b2552 0.25 -0.5
2,684,191 - hmp b2552 0.28 -0.6
2,684,191 - hmp b2552 0.28 -0.1
2,684,207 + hmp b2552 0.29 +1.1
2,684,222 + hmp b2552 0.31 +0.3
2,684,222 + hmp b2552 0.31 +0.1
2,684,330 + hmp b2552 0.40 -0.2
2,684,338 - hmp b2552 0.40 +0.3
2,684,338 - hmp b2552 0.40 +0.1
2,684,405 - hmp b2552 0.46 +0.4
2,684,476 - hmp b2552 0.52 -0.2
2,684,721 + hmp b2552 0.73 -0.7
2,684,789 + hmp b2552 0.78 +0.8
2,684,789 + hmp b2552 0.78 -0.4
2,684,827 - hmp b2552 0.81 +0.7
2,684,830 - hmp b2552 0.82 -0.2
2,684,970 + +0.3
2,684,970 + +0.4
2,685,032 + +1.0
2,685,047 - +0.6
2,685,079 + +0.3
2,685,107 + +0.1
2,685,107 + +1.6
2,685,120 + -1.3
2,685,120 + +0.9
2,685,223 - glnB b2553 0.39 +0.1
2,685,273 + glnB b2553 0.53 +1.3
2,685,273 + glnB b2553 0.53 +0.5
2,685,281 - glnB b2553 0.56 +0.5
2,685,288 + glnB b2553 0.58 +0.2
2,685,311 - glnB b2553 0.65 +0.1
2,685,311 - glnB b2553 0.65 +0.7
2,685,311 - glnB b2553 0.65 -0.5
2,685,311 - glnB b2553 0.65 +0.6
2,685,371 - glnB b2553 0.82 +0.6
2,685,488 + -0.2
2,685,488 + +0.4
2,685,525 - +0.2
2,685,682 + yfhA b2554 0.14 -1.7
2,685,703 + yfhA b2554 0.16 +0.5
2,685,703 + yfhA b2554 0.16 -1.4
2,685,725 + yfhA b2554 0.18 -1.0
2,685,821 - yfhA b2554 0.25 -0.4
2,685,821 - yfhA b2554 0.25 -0.4
2,685,863 - yfhA b2554 0.28 -1.6
2,685,885 - yfhA b2554 0.30 -1.5
2,685,885 - yfhA b2554 0.30 -0.8
2,685,897 - yfhA b2554 0.30 -1.6
2,686,064 + yfhA b2554 0.43 -0.5
2,686,079 + yfhA b2554 0.44 +0.9
2,686,089 + +1.5
2,686,091 + yfhA b2554 0.45 -0.1
2,686,091 + yfhA b2554 0.45 -1.1
2,686,158 - yfhA b2554 0.50 +0.4
2,686,162 - yfhA b2554 0.50 -0.2
2,686,250 + yfhA b2554 0.57 -0.4
2,686,282 + yfhA b2554 0.59 +0.1
2,686,294 - yfhA b2554 0.60 -0.5
2,686,323 + yfhA b2554 0.62 -0.1
2,686,323 + yfhA b2554 0.62 +0.1
2,686,331 - yfhA b2554 0.63 -0.4
2,686,425 - yfhA b2554 0.70 -0.8

Or see this region's nucleotide sequence