Strain Fitness in Escherichia coli BW25113 around b3390

Experiment: Putrescine (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntaroB and aroK are separated by 56 nucleotidesaroK and hofQ are separated by 400 nucleotides b3389: aroB - 3-dehydroquinate synthase (NCBI), at 3,515,420 to 3,516,508 aroB b3390: aroK - shikimate kinase I (NCBI), at 3,516,565 to 3,517,086 aroK b3391: hofQ - predicted fimbrial transporter (NCBI), at 3,517,487 to 3,518,725 hofQ Position (kb) 3516 3517 3518Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1 2at 3515.570 kb on - strand, within aroBat 3515.570 kb on - strand, within aroBat 3515.592 kb on + strand, within aroBat 3515.592 kb on + strand, within aroBat 3515.598 kb on + strand, within aroBat 3515.624 kb on + strand, within aroBat 3515.631 kb on - strand, within aroBat 3515.633 kb on - strand, within aroBat 3515.633 kb on - strand, within aroBat 3515.633 kb on - strand, within aroBat 3515.649 kb on + strand, within aroBat 3515.649 kb on + strand, within aroBat 3515.649 kb on + strand, within aroBat 3515.657 kb on - strand, within aroBat 3515.717 kb on - strand, within aroBat 3515.723 kb on - strand, within aroBat 3515.743 kb on - strand, within aroBat 3515.753 kb on - strand, within aroBat 3515.758 kb on + strand, within aroBat 3515.758 kb on + strand, within aroBat 3515.758 kb on + strand, within aroBat 3515.770 kb on + strand, within aroBat 3515.770 kb on + strand, within aroBat 3515.794 kb on - strand, within aroBat 3515.824 kb on + strand, within aroBat 3515.848 kb on + strand, within aroBat 3515.851 kb on + strand, within aroBat 3515.864 kb on - strand, within aroBat 3515.873 kb on + strand, within aroBat 3515.874 kb on - strand, within aroBat 3515.876 kb on + strand, within aroBat 3515.930 kb on + strand, within aroBat 3515.967 kb on - strand, within aroBat 3516.005 kb on + strand, within aroBat 3516.024 kb on + strand, within aroBat 3516.079 kb on - strand, within aroBat 3516.079 kb on - strand, within aroBat 3516.104 kb on + strand, within aroBat 3516.174 kb on - strand, within aroBat 3516.190 kb on + strand, within aroBat 3516.221 kb on + strand, within aroBat 3516.221 kb on + strand, within aroBat 3516.222 kb on - strand, within aroBat 3516.226 kb on - strand, within aroBat 3516.258 kb on - strand, within aroBat 3516.285 kb on - strand, within aroBat 3516.285 kb on - strand, within aroBat 3516.319 kb on + strand, within aroBat 3516.319 kb on + strand, within aroBat 3516.393 kb on + strandat 3516.402 kb on - strandat 3516.496 kb on - strandat 3516.501 kb on - strandat 3516.549 kb on + strandat 3516.554 kb on - strandat 3516.554 kb on - strandat 3516.554 kb on - strandat 3516.626 kb on + strand, within aroKat 3516.661 kb on - strand, within aroKat 3516.793 kb on + strand, within aroKat 3516.801 kb on + strand, within aroKat 3516.801 kb on + strand, within aroKat 3516.858 kb on - strand, within aroKat 3517.041 kb on - strandat 3517.046 kb on + strandat 3517.054 kb on - strandat 3517.066 kb on - strandat 3517.107 kb on - strandat 3517.144 kb on + strandat 3517.152 kb on - strandat 3517.164 kb on + strandat 3517.207 kb on + strandat 3517.224 kb on - strandat 3517.228 kb on + strandat 3517.247 kb on + strandat 3517.283 kb on - strandat 3517.360 kb on - strandat 3517.389 kb on - strandat 3517.426 kb on - strandat 3517.437 kb on + strandat 3517.441 kb on - strandat 3517.441 kb on - strandat 3517.530 kb on - strandat 3517.615 kb on - strand, within hofQat 3517.615 kb on - strand, within hofQat 3517.706 kb on + strand, within hofQat 3517.706 kb on + strand, within hofQat 3517.711 kb on + strand, within hofQat 3517.711 kb on + strand, within hofQat 3517.730 kb on + strand, within hofQat 3517.730 kb on + strand, within hofQat 3517.730 kb on + strand, within hofQat 3517.798 kb on + strand, within hofQat 3517.817 kb on + strand, within hofQat 3517.817 kb on - strand, within hofQat 3517.825 kb on - strand, within hofQat 3517.911 kb on + strand, within hofQat 3517.911 kb on + strand, within hofQat 3517.928 kb on - strand, within hofQat 3518.047 kb on + strand, within hofQ

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Per-strain Table

Position Strand Gene LocusTag Fraction Putrescine (N)
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3,515,570 - aroB b3389 0.14 -2.8
3,515,570 - aroB b3389 0.14 -1.5
3,515,592 + aroB b3389 0.16 -2.3
3,515,592 + aroB b3389 0.16 -3.2
3,515,598 + aroB b3389 0.16 -3.8
3,515,624 + aroB b3389 0.19 -2.1
3,515,631 - aroB b3389 0.19 -1.0
3,515,633 - aroB b3389 0.20 -1.8
3,515,633 - aroB b3389 0.20 +0.1
3,515,633 - aroB b3389 0.20 -3.7
3,515,649 + aroB b3389 0.21 -2.0
3,515,649 + aroB b3389 0.21 -2.4
3,515,649 + aroB b3389 0.21 -3.6
3,515,657 - aroB b3389 0.22 -3.4
3,515,717 - aroB b3389 0.27 -3.1
3,515,723 - aroB b3389 0.28 -3.2
3,515,743 - aroB b3389 0.30 -2.0
3,515,753 - aroB b3389 0.31 -2.7
3,515,758 + aroB b3389 0.31 -2.9
3,515,758 + aroB b3389 0.31 -2.8
3,515,758 + aroB b3389 0.31 -1.7
3,515,770 + aroB b3389 0.32 -2.2
3,515,770 + aroB b3389 0.32 -1.5
3,515,794 - aroB b3389 0.34 -3.2
3,515,824 + aroB b3389 0.37 -2.8
3,515,848 + aroB b3389 0.39 -3.3
3,515,851 + aroB b3389 0.40 -2.9
3,515,864 - aroB b3389 0.41 -1.5
3,515,873 + aroB b3389 0.42 -4.0
3,515,874 - aroB b3389 0.42 -4.3
3,515,876 + aroB b3389 0.42 -4.3
3,515,930 + aroB b3389 0.47 -2.8
3,515,967 - aroB b3389 0.50 -3.2
3,516,005 + aroB b3389 0.54 -4.4
3,516,024 + aroB b3389 0.55 -3.1
3,516,079 - aroB b3389 0.61 -2.4
3,516,079 - aroB b3389 0.61 -3.4
3,516,104 + aroB b3389 0.63 -4.2
3,516,174 - aroB b3389 0.69 -5.0
3,516,190 + aroB b3389 0.71 -3.6
3,516,221 + aroB b3389 0.74 -3.6
3,516,221 + aroB b3389 0.74 -3.9
3,516,222 - aroB b3389 0.74 -2.4
3,516,226 - aroB b3389 0.74 -2.2
3,516,258 - aroB b3389 0.77 -3.3
3,516,285 - aroB b3389 0.79 -2.6
3,516,285 - aroB b3389 0.79 +0.1
3,516,319 + aroB b3389 0.83 -3.0
3,516,319 + aroB b3389 0.83 -2.1
3,516,393 + -0.6
3,516,402 - -2.5
3,516,496 - -2.8
3,516,501 - -1.6
3,516,549 + -0.8
3,516,554 - -0.6
3,516,554 - -1.1
3,516,554 - -1.8
3,516,626 + aroK b3390 0.12 -2.5
3,516,661 - aroK b3390 0.18 -2.1
3,516,793 + aroK b3390 0.44 -0.8
3,516,801 + aroK b3390 0.45 -1.0
3,516,801 + aroK b3390 0.45 -1.3
3,516,858 - aroK b3390 0.56 -1.3
3,517,041 - -0.1
3,517,046 + -2.1
3,517,054 - -1.5
3,517,066 - -0.9
3,517,107 - -0.2
3,517,144 + +0.1
3,517,152 - -2.1
3,517,164 + -0.9
3,517,207 + -0.5
3,517,224 - -0.9
3,517,228 + +0.0
3,517,247 + -1.0
3,517,283 - -0.7
3,517,360 - -0.8
3,517,389 - -1.8
3,517,426 - -0.5
3,517,437 + -0.5
3,517,441 - -1.2
3,517,441 - -0.8
3,517,530 - -1.2
3,517,615 - hofQ b3391 0.10 -2.4
3,517,615 - hofQ b3391 0.10 -0.2
3,517,706 + hofQ b3391 0.18 -0.6
3,517,706 + hofQ b3391 0.18 -0.6
3,517,711 + hofQ b3391 0.18 +0.3
3,517,711 + hofQ b3391 0.18 +1.8
3,517,730 + hofQ b3391 0.20 -0.3
3,517,730 + hofQ b3391 0.20 -1.2
3,517,730 + hofQ b3391 0.20 -0.5
3,517,798 + hofQ b3391 0.25 -0.4
3,517,817 + hofQ b3391 0.27 -0.8
3,517,817 - hofQ b3391 0.27 -1.3
3,517,825 - hofQ b3391 0.27 -0.2
3,517,911 + hofQ b3391 0.34 -0.6
3,517,911 + hofQ b3391 0.34 +0.3
3,517,928 - hofQ b3391 0.36 -0.7
3,518,047 + hofQ b3391 0.45 +1.0

Or see this region's nucleotide sequence