Strain Fitness in Escherichia coli BW25113 around b4219

Experiment: Putrescine (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntytfL and msrA are separated by 322 nucleotidesmsrA and ytfM are separated by 205 nucleotides b4218: ytfL - predicted inner membrane protein (NCBI), at 4,437,895 to 4,439,238 ytfL b4219: msrA - methionine sulfoxide reductase A (NCBI), at 4,439,561 to 4,440,199 msrA b4220: ytfM - predicted outer membrane protein and surface antigen (NCBI), at 4,440,405 to 4,442,138 ytfM Position (kb) 4439 4440 4441Strain fitness (log2 ratio) -2 -1 0 1 2 3 4at 4438.568 kb on - strand, within ytfLat 4438.595 kb on - strand, within ytfLat 4438.595 kb on - strand, within ytfLat 4438.694 kb on - strand, within ytfLat 4438.702 kb on - strand, within ytfLat 4438.731 kb on + strand, within ytfLat 4438.739 kb on - strand, within ytfLat 4438.739 kb on - strand, within ytfLat 4438.755 kb on + strand, within ytfLat 4438.755 kb on + strand, within ytfLat 4438.762 kb on - strand, within ytfLat 4438.762 kb on - strand, within ytfLat 4438.775 kb on + strand, within ytfLat 4438.775 kb on + strand, within ytfLat 4438.826 kb on - strand, within ytfLat 4438.907 kb on + strand, within ytfLat 4438.907 kb on + strand, within ytfLat 4439.009 kb on + strand, within ytfLat 4439.049 kb on - strand, within ytfLat 4439.071 kb on - strand, within ytfLat 4439.201 kb on - strandat 4439.208 kb on + strandat 4439.212 kb on + strandat 4439.212 kb on + strandat 4439.226 kb on - strandat 4439.345 kb on + strandat 4439.345 kb on + strandat 4439.355 kb on - strandat 4439.370 kb on - strandat 4439.431 kb on - strandat 4439.431 kb on - strandat 4439.466 kb on - strandat 4439.688 kb on + strand, within msrAat 4439.739 kb on + strand, within msrAat 4439.759 kb on + strand, within msrAat 4439.759 kb on + strand, within msrAat 4439.764 kb on + strand, within msrAat 4439.872 kb on + strand, within msrAat 4439.937 kb on - strand, within msrAat 4439.937 kb on - strand, within msrAat 4440.009 kb on + strand, within msrAat 4440.090 kb on + strand, within msrAat 4440.120 kb on - strand, within msrAat 4440.131 kb on - strand, within msrAat 4440.143 kb on - strandat 4440.143 kb on - strandat 4440.279 kb on - strandat 4440.331 kb on + strandat 4440.331 kb on + strandat 4440.430 kb on + strandat 4440.435 kb on + strandat 4440.435 kb on + strandat 4440.435 kb on + strandat 4440.483 kb on - strandat 4440.483 kb on - strandat 4440.485 kb on - strandat 4440.582 kb on + strand, within ytfMat 4440.582 kb on - strand, within ytfMat 4440.675 kb on - strand, within ytfMat 4440.681 kb on + strand, within ytfMat 4440.698 kb on + strand, within ytfMat 4440.740 kb on + strand, within ytfMat 4440.741 kb on - strand, within ytfMat 4440.741 kb on - strand, within ytfMat 4440.770 kb on - strand, within ytfMat 4440.770 kb on - strand, within ytfMat 4440.798 kb on + strand, within ytfMat 4440.810 kb on + strand, within ytfMat 4440.810 kb on + strand, within ytfMat 4440.810 kb on + strand, within ytfMat 4440.810 kb on + strand, within ytfMat 4440.810 kb on + strand, within ytfMat 4440.818 kb on - strand, within ytfMat 4440.861 kb on + strand, within ytfMat 4440.864 kb on - strand, within ytfMat 4440.864 kb on - strand, within ytfMat 4440.935 kb on + strand, within ytfMat 4440.935 kb on + strand, within ytfMat 4440.948 kb on + strand, within ytfMat 4440.973 kb on + strand, within ytfMat 4440.976 kb on - strand, within ytfMat 4440.989 kb on + strand, within ytfMat 4440.996 kb on - strand, within ytfMat 4441.096 kb on + strand, within ytfMat 4441.144 kb on - strand, within ytfM

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Per-strain Table

Position Strand Gene LocusTag Fraction Putrescine (N)
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4,438,568 - ytfL b4218 0.50 +4.1
4,438,595 - ytfL b4218 0.52 +4.6
4,438,595 - ytfL b4218 0.52 +3.8
4,438,694 - ytfL b4218 0.59 +4.1
4,438,702 - ytfL b4218 0.60 +3.6
4,438,731 + ytfL b4218 0.62 +4.2
4,438,739 - ytfL b4218 0.63 +3.8
4,438,739 - ytfL b4218 0.63 +3.8
4,438,755 + ytfL b4218 0.64 +4.3
4,438,755 + ytfL b4218 0.64 +4.3
4,438,762 - ytfL b4218 0.65 +3.8
4,438,762 - ytfL b4218 0.65 +4.4
4,438,775 + ytfL b4218 0.65 +4.0
4,438,775 + ytfL b4218 0.65 +4.6
4,438,826 - ytfL b4218 0.69 +4.6
4,438,907 + ytfL b4218 0.75 +3.8
4,438,907 + ytfL b4218 0.75 +3.9
4,439,009 + ytfL b4218 0.83 +3.4
4,439,049 - ytfL b4218 0.86 +3.8
4,439,071 - ytfL b4218 0.88 +3.9
4,439,201 - +4.0
4,439,208 + +4.5
4,439,212 + +4.0
4,439,212 + +4.0
4,439,226 - +4.2
4,439,345 + +4.2
4,439,345 + +4.0
4,439,355 - -2.0
4,439,370 - -0.9
4,439,431 - +0.2
4,439,431 - +2.0
4,439,466 - +0.5
4,439,688 + msrA b4219 0.20 +0.7
4,439,739 + msrA b4219 0.28 +0.2
4,439,759 + msrA b4219 0.31 +0.5
4,439,759 + msrA b4219 0.31 +0.8
4,439,764 + msrA b4219 0.32 +0.2
4,439,872 + msrA b4219 0.49 +0.5
4,439,937 - msrA b4219 0.59 +0.5
4,439,937 - msrA b4219 0.59 +0.0
4,440,009 + msrA b4219 0.70 -0.2
4,440,090 + msrA b4219 0.83 -0.5
4,440,120 - msrA b4219 0.87 +0.6
4,440,131 - msrA b4219 0.89 +0.3
4,440,143 - +0.3
4,440,143 - +0.1
4,440,279 - +0.7
4,440,331 + -0.4
4,440,331 + -0.1
4,440,430 + +0.0
4,440,435 + -1.5
4,440,435 + -0.4
4,440,435 + -0.1
4,440,483 - -0.2
4,440,483 - +0.8
4,440,485 - +0.6
4,440,582 + ytfM b4220 0.10 +0.3
4,440,582 - ytfM b4220 0.10 +0.7
4,440,675 - ytfM b4220 0.16 +0.2
4,440,681 + ytfM b4220 0.16 -0.1
4,440,698 + ytfM b4220 0.17 -0.3
4,440,740 + ytfM b4220 0.19 +0.0
4,440,741 - ytfM b4220 0.19 +0.6
4,440,741 - ytfM b4220 0.19 -0.9
4,440,770 - ytfM b4220 0.21 +0.6
4,440,770 - ytfM b4220 0.21 +0.9
4,440,798 + ytfM b4220 0.23 -0.5
4,440,810 + ytfM b4220 0.23 -0.4
4,440,810 + ytfM b4220 0.23 -0.4
4,440,810 + ytfM b4220 0.23 +1.0
4,440,810 + ytfM b4220 0.23 +0.5
4,440,810 + ytfM b4220 0.23 +1.5
4,440,818 - ytfM b4220 0.24 -0.1
4,440,861 + ytfM b4220 0.26 -0.5
4,440,864 - ytfM b4220 0.26 -0.2
4,440,864 - ytfM b4220 0.26 +0.1
4,440,935 + ytfM b4220 0.31 +0.7
4,440,935 + ytfM b4220 0.31 +0.9
4,440,948 + ytfM b4220 0.31 -1.0
4,440,973 + ytfM b4220 0.33 -1.2
4,440,976 - ytfM b4220 0.33 +1.0
4,440,989 + ytfM b4220 0.34 -0.1
4,440,996 - ytfM b4220 0.34 +0.6
4,441,096 + ytfM b4220 0.40 -0.3
4,441,144 - ytfM b4220 0.43 -0.0

Or see this region's nucleotide sequence