Strain Fitness in Escherichia coli BW25113 around b4218

Experiment: Putrescine (N)

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	Putrescine (N)
4,436,902	-	ytfJ	b4216	0.31	-0.6
4,436,902	-	ytfJ	b4216	0.31	+1.0
4,436,929	+	ytfJ	b4216	0.36	+0.4
4,436,991	+	ytfJ	b4216	0.47	+0.5
4,437,003	+	ytfJ	b4216	0.49	-0.3
4,437,203	-	ytfJ	b4216	0.85	+1.1
4,437,233	+				+0.0
4,437,239	+				+0.7
4,437,247	+				+0.4
4,437,251	+				-0.3
4,437,281	+				+1.0
4,437,281	+				+0.7
4,437,282	-				+0.2
4,437,282	-				+0.1
4,437,287	+				+0.6
4,437,287	-				-0.3
4,437,289	-				-0.3
4,437,289	-				-0.0
4,437,289	-				-0.1
4,437,300	-				+0.2
4,437,316	-				-0.3
4,437,323	+				-0.2
4,437,363	-				-1.1
4,437,363	-				-1.1
4,437,395	+				+0.4
4,437,420	+				+1.4
4,437,420	+				+0.5
4,437,425	+				+1.4
4,437,519	+				+0.5
4,437,526	-				-0.3
4,437,526	-				+0.1
4,437,527	-				-0.2
4,437,527	-				+0.1
4,437,574	+				-0.0
4,437,635	+	ytfK	b4217	0.12	+0.4
4,437,635	+	ytfK	b4217	0.12	+0.3
4,437,660	-	ytfK	b4217	0.24	+0.2
4,437,686	-	ytfK	b4217	0.37	-0.2
4,437,686	-	ytfK	b4217	0.37	-0.5
4,437,692	-	ytfK	b4217	0.40	+1.1
4,437,717	+	ytfK	b4217	0.52	-0.3
4,437,768	+	ytfK	b4217	0.76	-0.0
4,437,768	+	ytfK	b4217	0.76	+0.2
4,437,786	+	ytfK	b4217	0.85	+0.5
4,437,788	-	ytfK	b4217	0.86	-0.2
4,437,858	-				-0.3
4,437,858	-				+0.3
4,437,860	+				+1.6
4,437,860	+				+0.7
4,437,946	+				+3.9
4,437,958	+				+4.1
4,437,976	-				+4.1
4,437,979	+				+4.0
4,437,997	+				+4.3
4,437,997	+				+4.8
4,438,030	+	ytfL	b4218	0.10	+4.1
4,438,071	+	ytfL	b4218	0.13	+4.2
4,438,072	-	ytfL	b4218	0.13	+4.3
4,438,081	-	ytfL	b4218	0.14	+4.1
4,438,081	-	ytfL	b4218	0.14	+3.6
4,438,226	-	ytfL	b4218	0.25	+4.0
4,438,226	-	ytfL	b4218	0.25	+3.9
4,438,281	+	ytfL	b4218	0.29	+3.6
4,438,355	-	ytfL	b4218	0.34	+4.0
4,438,355	-	ytfL	b4218	0.34	+3.6
4,438,369	-	ytfL	b4218	0.35	+3.9
4,438,390	+	ytfL	b4218	0.37	+3.6
4,438,390	+	ytfL	b4218	0.37	+4.2
4,438,408	-	ytfL	b4218	0.38	+4.2
4,438,408	-	ytfL	b4218	0.38	+4.3
4,438,431	+	ytfL	b4218	0.40	+4.3
4,438,438	+	ytfL	b4218	0.40	+4.0
4,438,444	-	ytfL	b4218	0.41	+4.0
4,438,458	+	ytfL	b4218	0.42	+3.5
4,438,458	+	ytfL	b4218	0.42	+4.6
4,438,474	-	ytfL	b4218	0.43	+4.1
4,438,510	+	ytfL	b4218	0.46	+4.5
4,438,527	+	ytfL	b4218	0.47	+3.0
4,438,568	-	ytfL	b4218	0.50	+4.1
4,438,595	-	ytfL	b4218	0.52	+4.6
4,438,595	-	ytfL	b4218	0.52	+3.8
4,438,694	-	ytfL	b4218	0.59	+4.1
4,438,702	-	ytfL	b4218	0.60	+3.6
4,438,731	+	ytfL	b4218	0.62	+4.2
4,438,739	-	ytfL	b4218	0.63	+3.8
4,438,739	-	ytfL	b4218	0.63	+3.8
4,438,755	+	ytfL	b4218	0.64	+4.3
4,438,755	+	ytfL	b4218	0.64	+4.3
4,438,762	-	ytfL	b4218	0.65	+3.8
4,438,762	-	ytfL	b4218	0.65	+4.4
4,438,775	+	ytfL	b4218	0.65	+4.0
4,438,775	+	ytfL	b4218	0.65	+4.6
4,438,826	-	ytfL	b4218	0.69	+4.6
4,438,907	+	ytfL	b4218	0.75	+3.8
4,438,907	+	ytfL	b4218	0.75	+3.9
4,439,009	+	ytfL	b4218	0.83	+3.4
4,439,049	-	ytfL	b4218	0.86	+3.8
4,439,071	-	ytfL	b4218	0.88	+3.9
4,439,201	-				+4.0
4,439,208	+				+4.5
4,439,212	+				+4.0
4,439,212	+				+4.0
4,439,226	-				+4.2
4,439,345	+				+4.2
4,439,345	+				+4.0
4,439,355	-				-2.0
4,439,370	-				-0.9
4,439,431	-				+0.2
4,439,431	-				+2.0
4,439,466	-				+0.5
4,439,688	+	msrA	b4219	0.20	+0.7
4,439,739	+	msrA	b4219	0.28	+0.2
4,439,759	+	msrA	b4219	0.31	+0.5
4,439,759	+	msrA	b4219	0.31	+0.8
4,439,764	+	msrA	b4219	0.32	+0.2
4,439,872	+	msrA	b4219	0.49	+0.5
4,439,937	-	msrA	b4219	0.59	+0.5
4,439,937	-	msrA	b4219	0.59	+0.0
4,440,009	+	msrA	b4219	0.70	-0.2
4,440,090	+	msrA	b4219	0.83	-0.5
4,440,120	-	msrA	b4219	0.87	+0.6
4,440,131	-	msrA	b4219	0.89	+0.3
4,440,143	-				+0.3
4,440,143	-				+0.1