Strain Fitness in Escherichia coli BW25113 around b3809

Experiment: Putrescine (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntcyaY and yifL are separated by 462 nucleotidesyifL and dapF are separated by 36 nucleotidesdapF and yigA overlap by 4 nucleotidesyigA and xerC overlap by 4 nucleotides b3807: cyaY - frataxin-like protein (NCBI), at 3,991,762 to 3,992,082 cyaY b4558: yifL - predicted lipoprotein (NCBI), at 3,992,545 to 3,992,748 yifL b3809: dapF - diaminopimelate epimerase (VIMSS), at 3,992,785 to 3,993,609 dapF b3810: yigA - hypothetical protein (NCBI), at 3,993,606 to 3,994,313 yigA b3811: xerC - site-specific tyrosine recombinase XerC (NCBI), at 3,994,310 to 3,995,206 xerC Position (kb) 3992 3993 3994Strain fitness (log2 ratio) -2 -1 0 1 2 3at 3991.789 kb on + strandat 3991.817 kb on + strand, within cyaYat 3991.837 kb on + strand, within cyaYat 3991.837 kb on + strand, within cyaYat 3991.912 kb on - strand, within cyaYat 3991.912 kb on - strand, within cyaYat 3991.912 kb on - strand, within cyaYat 3991.917 kb on - strand, within cyaYat 3991.957 kb on + strand, within cyaYat 3991.990 kb on + strand, within cyaYat 3991.990 kb on + strand, within cyaYat 3992.000 kb on - strand, within cyaYat 3992.000 kb on - strand, within cyaYat 3992.010 kb on - strand, within cyaYat 3992.084 kb on + strandat 3992.084 kb on + strandat 3992.114 kb on + strandat 3992.114 kb on + strandat 3992.114 kb on + strandat 3992.122 kb on - strandat 3992.122 kb on - strandat 3992.136 kb on - strandat 3992.143 kb on - strandat 3992.151 kb on - strandat 3992.213 kb on - strandat 3992.213 kb on - strandat 3992.283 kb on + strandat 3992.327 kb on - strandat 3992.343 kb on + strandat 3992.343 kb on + strandat 3992.361 kb on - strandat 3992.361 kb on - strandat 3992.371 kb on + strandat 3992.381 kb on - strandat 3992.418 kb on - strandat 3992.422 kb on + strandat 3992.422 kb on + strandat 3992.424 kb on + strandat 3992.432 kb on - strandat 3992.456 kb on + strandat 3992.456 kb on + strandat 3992.482 kb on + strandat 3992.527 kb on + strandat 3992.530 kb on - strandat 3992.572 kb on + strand, within yifLat 3992.572 kb on + strand, within yifLat 3992.572 kb on + strand, within yifLat 3992.578 kb on - strand, within yifLat 3992.578 kb on - strand, within yifLat 3992.578 kb on - strand, within yifLat 3992.586 kb on + strand, within yifLat 3992.605 kb on + strand, within yifLat 3992.653 kb on + strand, within yifLat 3992.693 kb on - strandat 3992.693 kb on - strand, within yifLat 3992.728 kb on + strandat 3992.728 kb on + strandat 3992.731 kb on + strandat 3992.738 kb on - strandat 3992.745 kb on + strandat 3992.761 kb on + strandat 3992.783 kb on - strandat 3992.790 kb on - strandat 3992.798 kb on - strandat 3993.601 kb on + strandat 3993.601 kb on + strandat 3993.637 kb on + strandat 3993.647 kb on - strandat 3993.778 kb on - strand, within yigAat 3993.778 kb on - strand, within yigAat 3993.782 kb on - strand, within yigAat 3993.813 kb on - strand, within yigAat 3993.813 kb on - strand, within yigAat 3993.815 kb on + strand, within yigAat 3993.827 kb on + strand, within yigAat 3993.827 kb on + strand, within yigAat 3993.829 kb on + strand, within yigAat 3993.837 kb on - strand, within yigAat 3993.837 kb on - strand, within yigAat 3993.854 kb on - strand, within yigAat 3993.892 kb on - strand, within yigAat 3993.892 kb on - strand, within yigAat 3993.948 kb on - strand, within yigAat 3993.948 kb on - strand, within yigAat 3993.972 kb on - strand, within yigAat 3993.978 kb on - strand, within yigAat 3994.051 kb on - strand, within yigAat 3994.088 kb on - strand, within yigAat 3994.090 kb on + strand, within yigAat 3994.090 kb on + strand, within yigAat 3994.093 kb on - strand, within yigAat 3994.098 kb on - strand, within yigAat 3994.098 kb on - strand, within yigAat 3994.098 kb on - strand, within yigAat 3994.098 kb on - strand, within yigAat 3994.098 kb on - strand, within yigAat 3994.098 kb on - strand, within yigAat 3994.170 kb on + strand, within yigAat 3994.192 kb on + strand, within yigAat 3994.192 kb on + strand, within yigAat 3994.202 kb on + strand, within yigAat 3994.210 kb on - strand, within yigAat 3994.279 kb on - strandat 3994.300 kb on - strandat 3994.300 kb on - strandat 3994.314 kb on - strandat 3994.318 kb on + strandat 3994.399 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Putrescine (N)
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3,991,789 + -0.9
3,991,817 + cyaY b3807 0.17 -1.1
3,991,837 + cyaY b3807 0.23 -1.4
3,991,837 + cyaY b3807 0.23 -0.6
3,991,912 - cyaY b3807 0.47 -1.3
3,991,912 - cyaY b3807 0.47 -0.3
3,991,912 - cyaY b3807 0.47 -0.7
3,991,917 - cyaY b3807 0.48 -1.1
3,991,957 + cyaY b3807 0.61 -0.7
3,991,990 + cyaY b3807 0.71 +0.6
3,991,990 + cyaY b3807 0.71 -0.7
3,992,000 - cyaY b3807 0.74 -0.7
3,992,000 - cyaY b3807 0.74 -0.6
3,992,010 - cyaY b3807 0.77 -1.2
3,992,084 + -0.9
3,992,084 + +0.2
3,992,114 + -0.7
3,992,114 + -0.8
3,992,114 + -1.1
3,992,122 - +0.1
3,992,122 - -0.6
3,992,136 - -1.7
3,992,143 - +1.3
3,992,151 - +0.3
3,992,213 - +0.3
3,992,213 - +0.1
3,992,283 + -0.2
3,992,327 - -0.6
3,992,343 + +0.0
3,992,343 + +0.4
3,992,361 - +3.7
3,992,361 - +3.5
3,992,371 + -0.1
3,992,381 - -0.2
3,992,418 - -0.2
3,992,422 + -1.3
3,992,422 + -2.3
3,992,424 + -1.5
3,992,432 - +0.3
3,992,456 + -0.6
3,992,456 + -0.4
3,992,482 + -0.6
3,992,527 + -0.4
3,992,530 - +0.4
3,992,572 + yifL b4558 0.13 -0.3
3,992,572 + yifL b4558 0.13 +0.4
3,992,572 + yifL b4558 0.13 -0.6
3,992,578 - yifL b4558 0.16 -1.6
3,992,578 - yifL b4558 0.16 -2.3
3,992,578 - yifL b4558 0.16 -1.9
3,992,586 + yifL b4558 0.20 +1.5
3,992,605 + yifL b4558 0.29 -0.0
3,992,653 + yifL b4558 0.53 -0.5
3,992,693 - +1.2
3,992,693 - yifL b4558 0.73 -0.8
3,992,728 + +0.7
3,992,728 + -0.8
3,992,731 + -0.7
3,992,738 - -0.7
3,992,745 + -0.6
3,992,761 + +0.4
3,992,783 - -0.0
3,992,790 - -0.5
3,992,798 - -0.5
3,993,601 + -0.2
3,993,601 + -0.3
3,993,637 + +0.4
3,993,647 - +0.3
3,993,778 - yigA b3810 0.24 -0.6
3,993,778 - yigA b3810 0.24 +0.4
3,993,782 - yigA b3810 0.25 +0.3
3,993,813 - yigA b3810 0.29 -0.7
3,993,813 - yigA b3810 0.29 +0.5
3,993,815 + yigA b3810 0.30 -1.0
3,993,827 + yigA b3810 0.31 +0.8
3,993,827 + yigA b3810 0.31 -0.5
3,993,829 + yigA b3810 0.31 +0.2
3,993,837 - yigA b3810 0.33 +0.2
3,993,837 - yigA b3810 0.33 +0.2
3,993,854 - yigA b3810 0.35 +1.5
3,993,892 - yigA b3810 0.40 +1.0
3,993,892 - yigA b3810 0.40 -0.9
3,993,948 - yigA b3810 0.48 -0.0
3,993,948 - yigA b3810 0.48 +0.2
3,993,972 - yigA b3810 0.52 +1.1
3,993,978 - yigA b3810 0.53 -1.1
3,994,051 - yigA b3810 0.63 +0.6
3,994,088 - yigA b3810 0.68 +0.3
3,994,090 + yigA b3810 0.68 +0.2
3,994,090 + yigA b3810 0.68 -0.1
3,994,093 - yigA b3810 0.69 +0.6
3,994,098 - yigA b3810 0.69 +0.1
3,994,098 - yigA b3810 0.69 +0.8
3,994,098 - yigA b3810 0.69 +0.6
3,994,098 - yigA b3810 0.69 -1.5
3,994,098 - yigA b3810 0.69 +0.4
3,994,098 - yigA b3810 0.69 -0.2
3,994,170 + yigA b3810 0.80 -0.8
3,994,192 + yigA b3810 0.83 -1.5
3,994,192 + yigA b3810 0.83 -1.1
3,994,202 + yigA b3810 0.84 -0.1
3,994,210 - yigA b3810 0.85 +0.1
3,994,279 - -0.6
3,994,300 - +0.5
3,994,300 - +0.8
3,994,314 - +0.1
3,994,318 + -0.6
3,994,399 + +0.2

Or see this region's nucleotide sequence