Experiment: Putrescine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt glgB and asd are separated by 272 nucleotides asd and yhgN are separated by 192 nucleotides yhgN and gntU are separated by 56 nucleotides
b3432: glgB - glycogen branching enzyme (NCBI), at 3,569,339 to 3,571,525
glgB
b3433: asd - aspartate-semialdehyde dehydrogenase (NCBI), at 3,571,798 to 3,572,901
asd
b3434: yhgN - predicted antibiotic transporter (NCBI), at 3,573,094 to 3,573,687
yhgN
b4476: gntU - gluconate transporter, low affinity GNT 1 system (NCBI), at 3,573,744 to 3,575,084
gntU
Position (kb)
3571
3572
3573 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3570.832 kb on + strand, within glgB at 3570.849 kb on - strand, within glgB at 3570.890 kb on + strand, within glgB at 3570.913 kb on - strand, within glgB at 3570.941 kb on + strand, within glgB at 3570.968 kb on - strand, within glgB at 3571.005 kb on - strand, within glgB at 3571.005 kb on - strand, within glgB at 3571.036 kb on + strand, within glgB at 3571.125 kb on - strand, within glgB at 3571.125 kb on - strand, within glgB at 3571.179 kb on - strand, within glgB at 3571.179 kb on - strand, within glgB at 3571.189 kb on + strand, within glgB at 3571.227 kb on + strand, within glgB at 3571.227 kb on + strand, within glgB at 3571.269 kb on + strand, within glgB at 3571.322 kb on - strand at 3571.322 kb on - strand at 3571.384 kb on + strand at 3571.388 kb on - strand at 3571.398 kb on - strand at 3571.510 kb on + strand at 3571.510 kb on + strand at 3571.546 kb on + strand at 3571.554 kb on - strand at 3571.599 kb on - strand at 3571.606 kb on + strand at 3571.614 kb on - strand at 3571.624 kb on - strand at 3571.634 kb on - strand at 3571.659 kb on + strand at 3571.688 kb on - strand at 3571.702 kb on - strand at 3571.764 kb on - strand at 3571.771 kb on - strand at 3571.771 kb on - strand at 3572.953 kb on - strand at 3572.953 kb on - strand at 3572.958 kb on - strand at 3572.961 kb on + strand at 3572.970 kb on - strand at 3573.020 kb on + strand at 3573.030 kb on - strand at 3573.033 kb on - strand at 3573.033 kb on - strand at 3573.034 kb on + strand at 3573.038 kb on + strand at 3573.056 kb on + strand at 3573.159 kb on - strand, within yhgN at 3573.159 kb on - strand, within yhgN at 3573.180 kb on - strand, within yhgN at 3573.212 kb on - strand, within yhgN at 3573.237 kb on + strand, within yhgN at 3573.237 kb on + strand, within yhgN at 3573.260 kb on - strand, within yhgN at 3573.305 kb on - strand, within yhgN at 3573.305 kb on - strand, within yhgN at 3573.313 kb on + strand, within yhgN at 3573.323 kb on + strand, within yhgN at 3573.373 kb on + strand, within yhgN at 3573.418 kb on - strand, within yhgN at 3573.468 kb on + strand, within yhgN at 3573.468 kb on + strand, within yhgN at 3573.469 kb on - strand, within yhgN at 3573.471 kb on + strand, within yhgN at 3573.488 kb on - strand, within yhgN at 3573.528 kb on - strand, within yhgN at 3573.569 kb on + strand, within yhgN at 3573.569 kb on + strand, within yhgN at 3573.582 kb on + strand, within yhgN at 3573.595 kb on - strand, within yhgN at 3573.595 kb on - strand, within yhgN at 3573.643 kb on + strand at 3573.643 kb on + strand at 3573.652 kb on + strand at 3573.683 kb on + strand at 3573.708 kb on + strand at 3573.710 kb on + strand at 3573.758 kb on - strand at 3573.782 kb on + strand at 3573.810 kb on - strand at 3573.848 kb on - strand at 3573.848 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Putrescine (N) remove 3,570,832 + glgB b3432 0.68 -2.0 3,570,849 - glgB b3432 0.69 -2.2 3,570,890 + glgB b3432 0.71 -1.6 3,570,913 - glgB b3432 0.72 -3.3 3,570,941 + glgB b3432 0.73 -2.7 3,570,968 - glgB b3432 0.74 +0.1 3,571,005 - glgB b3432 0.76 +1.1 3,571,005 - glgB b3432 0.76 -2.9 3,571,036 + glgB b3432 0.78 -2.5 3,571,125 - glgB b3432 0.82 -1.8 3,571,125 - glgB b3432 0.82 -1.6 3,571,179 - glgB b3432 0.84 -1.7 3,571,179 - glgB b3432 0.84 -0.9 3,571,189 + glgB b3432 0.85 -1.1 3,571,227 + glgB b3432 0.86 -1.5 3,571,227 + glgB b3432 0.86 -0.8 3,571,269 + glgB b3432 0.88 -0.1 3,571,322 - -1.9 3,571,322 - +0.3 3,571,384 + -2.7 3,571,388 - -0.3 3,571,398 - -0.7 3,571,510 + -0.9 3,571,510 + -2.6 3,571,546 + +0.1 3,571,554 - +1.6 3,571,599 - +1.6 3,571,606 + -1.9 3,571,614 - +1.1 3,571,624 - +1.5 3,571,634 - +1.4 3,571,659 + -0.7 3,571,688 - +2.0 3,571,702 - +1.8 3,571,764 - +1.3 3,571,771 - +1.3 3,571,771 - +1.5 3,572,953 - +0.6 3,572,953 - +0.6 3,572,958 - +0.6 3,572,961 + -1.6 3,572,970 - +1.2 3,573,020 + -1.0 3,573,030 - +0.8 3,573,033 - -0.1 3,573,033 - +1.0 3,573,034 + +0.7 3,573,038 + -0.2 3,573,056 + -1.3 3,573,159 - yhgN b3434 0.11 +0.5 3,573,159 - yhgN b3434 0.11 +0.9 3,573,180 - yhgN b3434 0.14 +0.4 3,573,212 - yhgN b3434 0.20 +0.2 3,573,237 + yhgN b3434 0.24 +1.4 3,573,237 + yhgN b3434 0.24 +0.2 3,573,260 - yhgN b3434 0.28 +0.3 3,573,305 - yhgN b3434 0.36 -1.0 3,573,305 - yhgN b3434 0.36 -0.2 3,573,313 + yhgN b3434 0.37 +0.3 3,573,323 + yhgN b3434 0.39 +0.2 3,573,373 + yhgN b3434 0.47 -1.0 3,573,418 - yhgN b3434 0.55 -0.1 3,573,468 + yhgN b3434 0.63 -0.2 3,573,468 + yhgN b3434 0.63 -0.8 3,573,469 - yhgN b3434 0.63 +0.2 3,573,471 + yhgN b3434 0.63 -0.3 3,573,488 - yhgN b3434 0.66 +0.2 3,573,528 - yhgN b3434 0.73 +0.8 3,573,569 + yhgN b3434 0.80 +0.7 3,573,569 + yhgN b3434 0.80 -0.7 3,573,582 + yhgN b3434 0.82 -0.1 3,573,595 - yhgN b3434 0.84 +0.9 3,573,595 - yhgN b3434 0.84 +0.3 3,573,643 + -0.6 3,573,643 + -0.1 3,573,652 + -0.1 3,573,683 + -0.7 3,573,708 + -0.6 3,573,710 + +0.1 3,573,758 - +0.5 3,573,782 + +0.2 3,573,810 - -0.6 3,573,848 - +0.3 3,573,848 - +0.7
Or see this region's nucleotide sequence