Experiment: Putrescine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt tdcG and yhaR are separated by 71 nucleotides yhaR and tdcE are separated by 13 nucleotides
b4471: tdcG - L-serine dehydratase 3 (NCBI), at 3,256,307 to 3,257,671
tdcG
b3113: yhaR - orf, hypothetical protein (VIMSS), at 3,257,743 to 3,258,132
yhaR
b3114: tdcE - pyruvate formate-lyase 4/2-ketobutyrate formate-lyase (NCBI), at 3,258,146 to 3,260,440
tdcE
Position (kb)
3257
3258
3259 Strain fitness (log2 ratio)
-2
-1
0
1 at 3256.768 kb on + strand, within tdcG at 3256.776 kb on - strand, within tdcG at 3256.811 kb on + strand, within tdcG at 3256.832 kb on - strand, within tdcG at 3256.832 kb on - strand, within tdcG at 3257.003 kb on - strand, within tdcG at 3257.004 kb on - strand, within tdcG at 3257.037 kb on - strand, within tdcG at 3257.106 kb on + strand, within tdcG at 3257.106 kb on + strand, within tdcG at 3257.114 kb on - strand, within tdcG at 3257.114 kb on - strand, within tdcG at 3257.196 kb on + strand, within tdcG at 3257.196 kb on + strand, within tdcG at 3257.238 kb on + strand, within tdcG at 3257.260 kb on - strand, within tdcG at 3257.260 kb on - strand, within tdcG at 3257.281 kb on - strand, within tdcG at 3257.281 kb on - strand, within tdcG at 3257.322 kb on - strand, within tdcG at 3257.402 kb on - strand, within tdcG at 3257.419 kb on - strand, within tdcG at 3257.436 kb on + strand, within tdcG at 3257.516 kb on - strand, within tdcG at 3257.516 kb on - strand, within tdcG at 3257.517 kb on + strand, within tdcG at 3257.536 kb on + strand at 3257.544 kb on - strand at 3257.544 kb on - strand at 3257.544 kb on - strand at 3257.544 kb on - strand at 3257.608 kb on + strand at 3257.608 kb on + strand at 3257.622 kb on - strand at 3257.695 kb on + strand at 3257.716 kb on + strand at 3257.745 kb on + strand at 3257.748 kb on - strand at 3257.813 kb on + strand, within yhaR at 3257.825 kb on + strand, within yhaR at 3257.825 kb on + strand, within yhaR at 3257.865 kb on - strand, within yhaR at 3257.937 kb on + strand, within yhaR at 3257.937 kb on + strand, within yhaR at 3257.957 kb on - strand, within yhaR at 3258.052 kb on - strand, within yhaR at 3258.054 kb on - strand, within yhaR at 3258.059 kb on - strand, within yhaR at 3258.086 kb on - strand, within yhaR at 3258.192 kb on + strand at 3258.202 kb on + strand at 3258.202 kb on + strand at 3258.220 kb on - strand at 3258.220 kb on - strand at 3258.314 kb on - strand at 3258.372 kb on + strand at 3258.428 kb on - strand, within tdcE at 3258.437 kb on - strand, within tdcE at 3258.620 kb on - strand, within tdcE at 3258.620 kb on - strand, within tdcE at 3258.633 kb on - strand, within tdcE at 3258.636 kb on + strand, within tdcE at 3258.641 kb on + strand, within tdcE at 3258.689 kb on + strand, within tdcE at 3258.758 kb on - strand, within tdcE at 3258.758 kb on - strand, within tdcE at 3258.785 kb on + strand, within tdcE at 3258.808 kb on - strand, within tdcE at 3258.808 kb on - strand, within tdcE at 3258.934 kb on + strand, within tdcE at 3258.934 kb on + strand, within tdcE at 3258.936 kb on + strand, within tdcE at 3258.936 kb on + strand, within tdcE at 3258.959 kb on - strand, within tdcE at 3258.964 kb on - strand, within tdcE at 3259.013 kb on + strand, within tdcE at 3259.013 kb on + strand, within tdcE at 3259.060 kb on - strand, within tdcE at 3259.070 kb on + strand, within tdcE
Per-strain Table
Position Strand Gene LocusTag Fraction Putrescine (N) remove 3,256,768 + tdcG b4471 0.34 -0.4 3,256,776 - tdcG b4471 0.34 -0.0 3,256,811 + tdcG b4471 0.37 -0.7 3,256,832 - tdcG b4471 0.38 +1.5 3,256,832 - tdcG b4471 0.38 +0.1 3,257,003 - tdcG b4471 0.51 +0.0 3,257,004 - tdcG b4471 0.51 -1.9 3,257,037 - tdcG b4471 0.53 -0.7 3,257,106 + tdcG b4471 0.59 -0.3 3,257,106 + tdcG b4471 0.59 -0.2 3,257,114 - tdcG b4471 0.59 +0.9 3,257,114 - tdcG b4471 0.59 -0.2 3,257,196 + tdcG b4471 0.65 -1.0 3,257,196 + tdcG b4471 0.65 -1.0 3,257,238 + tdcG b4471 0.68 +0.4 3,257,260 - tdcG b4471 0.70 -0.3 3,257,260 - tdcG b4471 0.70 +0.1 3,257,281 - tdcG b4471 0.71 +0.9 3,257,281 - tdcG b4471 0.71 -0.0 3,257,322 - tdcG b4471 0.74 -0.2 3,257,402 - tdcG b4471 0.80 +0.1 3,257,419 - tdcG b4471 0.81 -0.3 3,257,436 + tdcG b4471 0.83 -0.5 3,257,516 - tdcG b4471 0.89 +0.3 3,257,516 - tdcG b4471 0.89 -0.0 3,257,517 + tdcG b4471 0.89 +0.7 3,257,536 + +0.8 3,257,544 - +0.1 3,257,544 - -1.5 3,257,544 - -0.3 3,257,544 - +0.2 3,257,608 + +0.7 3,257,608 + -0.4 3,257,622 - -0.2 3,257,695 + +0.2 3,257,716 + -0.2 3,257,745 + +0.2 3,257,748 - +0.5 3,257,813 + yhaR b3113 0.18 +0.5 3,257,825 + yhaR b3113 0.21 -1.0 3,257,825 + yhaR b3113 0.21 +0.1 3,257,865 - yhaR b3113 0.31 +0.1 3,257,937 + yhaR b3113 0.50 -1.5 3,257,937 + yhaR b3113 0.50 +0.9 3,257,957 - yhaR b3113 0.55 -0.2 3,258,052 - yhaR b3113 0.79 +0.3 3,258,054 - yhaR b3113 0.80 +0.2 3,258,059 - yhaR b3113 0.81 +0.0 3,258,086 - yhaR b3113 0.88 +0.3 3,258,192 + -0.3 3,258,202 + +0.5 3,258,202 + +0.1 3,258,220 - +0.6 3,258,220 - +0.6 3,258,314 - -0.4 3,258,372 + +0.6 3,258,428 - tdcE b3114 0.12 -0.6 3,258,437 - tdcE b3114 0.13 -0.2 3,258,620 - tdcE b3114 0.21 -0.4 3,258,620 - tdcE b3114 0.21 +0.2 3,258,633 - tdcE b3114 0.21 -0.4 3,258,636 + tdcE b3114 0.21 +0.3 3,258,641 + tdcE b3114 0.22 +0.0 3,258,689 + tdcE b3114 0.24 +0.0 3,258,758 - tdcE b3114 0.27 -0.2 3,258,758 - tdcE b3114 0.27 -0.2 3,258,785 + tdcE b3114 0.28 +1.3 3,258,808 - tdcE b3114 0.29 +0.2 3,258,808 - tdcE b3114 0.29 -0.4 3,258,934 + tdcE b3114 0.34 +1.0 3,258,934 + tdcE b3114 0.34 +0.2 3,258,936 + tdcE b3114 0.34 +0.8 3,258,936 + tdcE b3114 0.34 -0.2 3,258,959 - tdcE b3114 0.35 +0.3 3,258,964 - tdcE b3114 0.36 +0.0 3,259,013 + tdcE b3114 0.38 +0.1 3,259,013 + tdcE b3114 0.38 -0.2 3,259,060 - tdcE b3114 0.40 -0.5 3,259,070 + tdcE b3114 0.40 +0.1
Or see this region's nucleotide sequence